| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,669,838 | T→C | 20.0% | V92A (GTT→GCT) | hcaC → | 3‑phenylpropionate dioxygenase, predicted ferredoxin subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,669,838 | 0 | T | C | 20.0% | 122.6 / 27.6 | 60 | V92A (GTT→GCT) | hcaC | 3‑phenylpropionate dioxygenase, predicted ferredoxin subunit |
| Reads supporting (aligned to +/- strand): ref base T (3/45); new base C (10/2); total (13/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.24e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.12e-01 | |||||||||||
TTTGCCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGA > W3110S.gb/2669769‑2669888 | tttGCCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGtt > 1:1421831/1‑71 (MQ=255)tttGCCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGtt > 1:11618/1‑71 (MQ=255) gCCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTg > 1:2854396/1‑69 (MQ=255) gCCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTg > 1:1595849/1‑69 (MQ=255) aaGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTc < 1:2649662/71‑1 (MQ=255) aaGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTc < 1:1298756/71‑1 (MQ=255) aaGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTc < 1:3037676/71‑1 (MQ=255) aaGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTc < 1:1840115/71‑1 (MQ=255) aaGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTc < 1:51258/71‑1 (MQ=255) aaGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTc < 1:1480627/71‑1 (MQ=255) aaGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTc < 1:157119/71‑1 (MQ=255) aaGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTc < 1:9558/71‑1 (MQ=255) cGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATc < 1:2606361/71‑1 (MQ=255) cGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATc < 1:559271/71‑1 (MQ=255) cGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATc < 1:2179159/71‑1 (MQ=255) cGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATc < 1:1883753/71‑1 (MQ=255) cGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATc < 1:3253586/71‑1 (MQ=255) cGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATc < 1:3305812/71‑1 (MQ=255) cGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATc < 1:3345008/71‑1 (MQ=255) cGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATc < 1:353331/71‑1 (MQ=255) cGTTATGCCTGCCCGCCACCGATCCGATCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATc < 1:1630095/71‑1 (MQ=255) gTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGTTGACATTTTCATc < 1:2850649/70‑1 (MQ=255) ttATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGa < 1:1967792/71‑1 (MQ=255) ttATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGa < 1:1980692/71‑1 (MQ=255) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGaa > 1:1468978/1‑49 (MQ=255) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGaa > 1:2539597/1‑49 (MQ=255) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGaa > 1:436448/1‑49 (MQ=255) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGaa > 1:2739160/1‑49 (MQ=255) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGaa > 1:2778038/1‑49 (MQ=255) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGaa > 1:1315510/1‑49 (MQ=255) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGaa > 1:2023138/1‑49 (MQ=255) aTGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGCTGaa > 1:132338/1‑49 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACtt < 1:2781980/71‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACtt < 1:722863/71‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACtt < 1:1215624/71‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACtt < 1:1271370/71‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACtt < 1:1327519/71‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACtt < 1:860309/71‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACtt < 1:1391255/71‑1 (MQ=255) tgcctgccCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGGGGTGACATTTTCATCGACtt < 1:1337752/71‑1 (MQ=255) gCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGgc < 1:609132/71‑1 (MQ=255) gCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGgc < 1:836042/71‑1 (MQ=255) gCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGgc < 1:2913677/71‑1 (MQ=255) gCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGgc < 1:185384/71‑1 (MQ=255) cGATCCGCTCACCACTTCTCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACtt < 1:3330984/57‑1 (MQ=255) cGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGc < 1:2767997/71‑1 (MQ=255) cGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGc < 1:2886458/71‑1 (MQ=255) cGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGc < 1:330873/71‑1 (MQ=255) ccGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCAt > 1:2381187/1‑70 (MQ=255) ccGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAg > 1:3049595/1‑60 (MQ=255) ccGCTCACCACTTATCCAGTACACGCTGAAGGTGGTGACATTTTCATCGACTTACCGGAg > 1:1872299/1‑60 (MQ=255) cGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGc < 1:2430510/66‑1 (MQ=255) caccacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCTGc < 1:3214002/62‑1 (MQ=255) accacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGa < 1:956981/70‑1 (MQ=255) accacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGa < 1:3231882/70‑1 (MQ=255) accacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGa < 1:2271703/70‑1 (MQ=255) accacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGa < 1:2211721/70‑1 (MQ=255) accacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGa < 1:2117043/70‑1 (MQ=255) accacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGa < 1:1678385/70‑1 (MQ=255) accacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGa < 1:1638898/70‑1 (MQ=255) ccacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGa < 1:2458628/69‑1 (MQ=255) ccacTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGa < 1:183864/69‑1 (MQ=255) | TTTGCCTGAAAACGGGGAAAGCGTTATGCCTGCCCGCCACCGATCCGCTCACCACTTATCCAGTACACGTTGAAGGTGGTGACATTTTCATCGACTTACCGGAGGCGCAGCCATGAGCGA > W3110S.gb/2669769‑2669888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |