| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,773,435 | T→C | 58.7% | L488L (TTG→CTG) | yfjW → | predicted inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,773,435 | 0 | T | C | 58.7% | 23.4 / 52.0 | 46 | L488L (TTG→CTG) | yfjW | predicted inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (5/14); new base C (19/8); total (24/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.34e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.16e-01 | |||||||||||
TAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTG > W3110S.gb/2773371‑2773501 | tAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATg > 1:1233554/1‑71 (MQ=255)tAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATg > 1:2181266/1‑71 (MQ=255)tAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATg > 1:214655/1‑71 (MQ=255)tAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATg > 1:697434/1‑71 (MQ=255)tAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATg > 1:562025/1‑71 (MQ=255) aGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa > 1:1876501/1‑68 (MQ=255) aGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa > 1:2873433/1‑68 (MQ=255) aGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa > 1:2951477/1‑68 (MQ=255) aGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa > 1:1981438/1‑68 (MQ=255) aGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa > 1:3350930/1‑68 (MQ=255) aGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa > 1:61208/1‑68 (MQ=255) aGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa > 1:847320/1‑68 (MQ=255) aGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa > 1:1318327/1‑68 (MQ=255) aGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTa > 1:1287595/1‑68 (MQ=255) cGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGt > 1:2898846/1‑69 (MQ=255) gAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg < 1:445082/59‑1 (MQ=255) gAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg < 1:488285/59‑1 (MQ=255) gAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg < 1:2749756/59‑1 (MQ=255) gAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg < 1:3326105/59‑1 (MQ=255) gAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg < 1:3152161/59‑1 (MQ=255) gAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg < 1:3067423/59‑1 (MQ=255) gAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg < 1:909941/59‑1 (MQ=255) gAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg < 1:2896984/59‑1 (MQ=255) gAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg < 1:281743/59‑1 (MQ=255) gAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg < 1:2486172/59‑1 (MQ=255) gAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg < 1:1850945/59‑1 (MQ=255) gAAACATTTGTCGTTAAGGACAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATg < 1:950508/59‑1 (MQ=255) aaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGa > 1:3277332/1‑59 (MQ=255) aaCATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGa > 1:547098/1‑59 (MQ=255) tGTCGTTAAGGCCAGAATCGGGGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCaa > 1:2098377/1‑70 (MQ=255) tGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTCTGTATGGACTAGTTAATAAAAGCaa > 1:233722/1‑70 (MQ=255) ggAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGca > 1:2728294/1‑71 (MQ=255) ggAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGca > 1:1683598/1‑71 (MQ=255) ggAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGca > 1:2327563/1‑71 (MQ=255) attattTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCAcagcag < 1:1855337/70‑1 (MQ=255) attattTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCAcagcag < 1:1763813/70‑1 (MQ=255) ttattTCTGAAGGGAGTCTGTATGGACTAGTtaataa < 1:1229407/37‑1 (MQ=255) ttattTCTGAAGGGAGTCTGTATGGACTAGTtaataa < 1:3327526/37‑1 (MQ=255) ttattTCTGAAGGGAGTCTGTATGGACTAGTtaataa < 1:1941270/37‑1 (MQ=255) ttattTCTGAAGGGAGTCTGTATGGACTAGTtaataa < 1:196591/37‑1 (MQ=255) ttattTCTGAAGGGAGTCTGTATGGACTAGTtaataa < 1:1752332/37‑1 (MQ=255) ttattTCTGAAGGGAGTCTGTATGGACTAGTtaataa < 1:680108/37‑1 (MQ=255) ttattTCTGAAGGGAGTCTGTATGGACTAGTtaataa < 1:237229/37‑1 (MQ=255) ttattTCTGAAGGGAGTCTGTATGGACTAGTtaataa < 1:1520646/37‑1 (MQ=255) gAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatata > 1:1765066/1‑45 (MQ=255) gAGTCTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAatata > 1:13255/1‑45 (MQ=255) tttGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTttgttg < 1:661063/68‑1 (MQ=255) tttGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTttgttg < 1:2757888/68‑1 (MQ=255) | TAGTATCAACGGGAAACATTTGTCGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTG > W3110S.gb/2773371‑2773501 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |