| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,794,102 | G→A | 48.1% | L398L (TTG→TTA) | gabP → | gamma‑aminobutyrate transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,794,102 | 0 | G | A | 48.1% | 8.3 / 114.3 | 82 | L398L (TTG→TTA) | gabP | gamma‑aminobutyrate transporter |
| Reads supporting (aligned to +/- strand): ref base G (13/29); new base A (18/21); total (31/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTT > W3110S.gb/2794035‑2794155 | taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCg < 1:1346851/70‑1 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCg < 1:1362331/70‑1 (MQ=255)taGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCg < 1:3077797/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGaa < 1:3157199/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGaa < 1:3337477/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGaa < 1:3031360/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGaa < 1:693704/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGaa < 1:1499239/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGaa < 1:2384428/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGaa < 1:2635583/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGaa < 1:2549683/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGaa < 1:2679970/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGaa < 1:2060722/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGaa < 1:1927517/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGaa < 1:692963/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGaa < 1:1691318/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGaa < 1:258576/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGaa < 1:5755/70‑1 (MQ=255) gTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGaa < 1:1107399/70‑1 (MQ=255) tCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCg < 1:332592/67‑1 (MQ=255) tCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCg < 1:72752/67‑1 (MQ=255) tCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCg < 1:1935699/67‑1 (MQ=255) gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCt > 1:2450257/1‑71 (MQ=255) gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCt > 1:2647429/1‑71 (MQ=255) gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCt > 1:930859/1‑71 (MQ=255) gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCt > 1:1402680/1‑71 (MQ=255) gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCt > 1:1460402/1‑71 (MQ=255) gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCt > 1:3259259/1‑71 (MQ=255) gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCt > 1:2832988/1‑71 (MQ=255) gCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCt > 1:2207281/1‑71 (MQ=255) gCCGATTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAAtg > 1:2630336/1‑66 (MQ=255) tCACCGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCTTTATcc > 1:345275/1‑71 (MQ=255) tCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCTTTATcc > 1:2386863/1‑71 (MQ=255) tCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCTTTATcc > 1:1338652/1‑71 (MQ=255) tCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCTTTATcc > 1:1470329/1‑71 (MQ=255) tCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCTTTATcc > 1:402431/1‑71 (MQ=255) tCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCTTTATcc > 1:332400/1‑71 (MQ=255) tCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCTTTATcc > 1:197439/1‑71 (MQ=255) tCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCTTTATc > 1:2868238/1‑70 (MQ=255) tCACAGTTGCGGATGCGCAAAATTATGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCTTTATcc > 1:469529/1‑71 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCt < 1:1604409/59‑1 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCt < 1:2566747/59‑1 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCt < 1:2512347/59‑1 (MQ=255) ttGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCt < 1:2210573/59‑1 (MQ=255) tGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTACGAATGTGGCt < 1:777913/58‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGTTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:3261658/71‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:3131632/71‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:298945/71‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:3269428/71‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:1962265/71‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:461082/71‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:2843803/71‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:2789006/71‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:1527553/71‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:2519059/71‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:2493084/71‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:2302798/71‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:1941034/71‑1 (MQ=255) cGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:2092322/71‑1 (MQ=255) ggATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCAc < 1:2943954/70‑1 (MQ=255) gAGCAGAAGGAAGCGAAAATTCGCTTTCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt < 1:2663747/71‑1 (MQ=255) gAGCAGAAGGAAGCGAAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt < 1:501741/71‑1 (MQ=255) gAGCAGAAGGAAGCGAAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt < 1:613355/71‑1 (MQ=255) gAGCAGAAGGAAGCGAAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt < 1:702201/71‑1 (MQ=255) gAGCAGAAGGAAGCGAAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt < 1:1328480/71‑1 (MQ=255) gAGCAGAAGGAAGCGAAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt < 1:2890043/71‑1 (MQ=255) gAGCAGAAGGAAGCGAAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt < 1:1385132/71‑1 (MQ=255) gAGCAGAAGGAAGCGAAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt < 1:2619519/71‑1 (MQ=255) gAGCAGAAGGAAGCGAAAATTCGCTTACGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCtt < 1:2442269/71‑1 (MQ=255) ggaagCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTttatta > 1:2903609/1‑67 (MQ=255) ggaagCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTttatta > 1:2730738/1‑67 (MQ=255) ggaagCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTttatta > 1:1554576/1‑67 (MQ=255) ggaagCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt > 1:626617/1‑71 (MQ=255) ggaagCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt > 1:968472/1‑71 (MQ=255) ggaagCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt > 1:858839/1‑71 (MQ=255) ggaagCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt > 1:1705052/1‑71 (MQ=255) ggaagCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt > 1:2112162/1‑71 (MQ=255) ggaagCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt > 1:3086882/1‑71 (MQ=255) ggaagCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt > 1:286545/1‑71 (MQ=255) ggaagCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt > 1:2881848/1‑71 (MQ=255) ggaagCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCtt > 1:3018197/1‑71 (MQ=255) ggaagCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCAGGCTAGTCATCGGCTTTATTACCtt > 1:1907640/1‑71 (MQ=255) | TAGTCATCGCCGTTTCACAGTTGCGGATGCGCAAAATTCTGCGAGCAGAAGGAAGCG‑AAATTCGCTTGCGAATGTGGCTTTATCCGTGGCTCACCTGGCTAGTCATCGGCTTTATTACCTT > W3110S.gb/2794035‑2794155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |