Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	2,795,805	G→T	55.1%	intergenic (‑121/‑62)	yqaE ← / → ygaV	predicted membrane protein/predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,795,805	0	G	T	55.1%	‑2.2 / 41.1	29	intergenic (‑121/‑62)	yqaE/ygaV	predicted membrane protein/predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (7/6);  new base T (6/10);  total (13/16)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

TTAGCGGAGCCTGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTTAAATAA  >  W3110S.gb/2795751‑2795864                                                       |                                                            ttAGCGGAGCCTGCCAGTTTCAGCGTACGCTTTTACTCTCATTTACTCTGCTGATGCTGGTATATGTTGc                                              >  1:1187297/1‑70 (MQ=255) ttAGCGGAGCCTGCCAGTTTCAGCGTACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCa                                             >  1:2771817/1‑71 (MQ=255) ttAGCGGAGCCTGCCAGTTTCAGCGTACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCa                                             >  1:2641764/1‑71 (MQ=255) ttAGCGGAGCCTGCCAGTTTCAGCGTACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCa                                             >  1:2555445/1‑71 (MQ=255) ttAGCGGAGCCTGCCAGTTTCAGCGTACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCa                                             >  1:3146191/1‑71 (MQ=255) ttAGCGGAGCCTGCCAGTTTCAGCGTACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCa                                             >  1:180802/1‑71 (MQ=255)                          tACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCACACTTAGTTTAGAGTTTACTTAAtt                    <  1:1209007/71‑1 (MQ=255)                          tACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCACACTTAGTTTAGAGTTTACTTAAtt                    <  1:2703589/71‑1 (MQ=255)                          tACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCACACTTAGTTTAGAGTTTACTTAAtt                    <  1:3163472/71‑1 (MQ=255)                          tACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCACACTTAGTTTAGAGTTTACTTAAtt                    <  1:2351816/71‑1 (MQ=255)                          tACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCACACTTAGTTTAGAGTTTACTTAAtt                    <  1:216908/71‑1 (MQ=255)                          tACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCACACTTAGTTTAGAGTTTACTTAAtt                    <  1:3322333/71‑1 (MQ=255)                          tACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCACACTTAGTTTAGAGTTTACTTAAtt                    <  1:534632/71‑1 (MQ=255)                          tACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCACACTTAGTTTAGAGTTTACTTAAtt                    <  1:1810423/71‑1 (MQ=255)                          tACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCACACTTAGTTTAGAGTTTACTTAAtt                    <  1:1640793/71‑1 (MQ=255)                          tACGCTTTTACTCTCATTTACGCTGCTGATGCTGGTATATGTTGCACACTTAGTTTAGAGTTTACTTAAtt                    <  1:1238513/71‑1 (MQ=255)                                         aTTTACGCTGCTGAGGCTGGTATATGTTGCTTTTTTAGTTTAGAGTTTa                           >  1:3101287/1‑49 (MQ=255)                                         aTTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTaga                                >  1:1404095/1‑44 (MQ=255)                                         aTTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGttt                            >  1:1392990/1‑48 (MQ=255)                                         aTTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTa                           >  1:617252/1‑49 (MQ=255)                                         aTTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTa                           >  1:2426384/1‑49 (MQ=255)                                         aTTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTa                           >  1:2423058/1‑49 (MQ=255)                                         aTTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTa                           >  1:1838481/1‑49 (MQ=255)                                            tACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTtaaata   <  1:245359/70‑1 (MQ=255)                                            tACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTtaaata   <  1:1997510/70‑1 (MQ=255)                                             aCGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTtaaataa  <  1:3102745/70‑1 (MQ=255)                                             aCGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTtaaataa  <  1:423777/70‑1 (MQ=255)                                             aCGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTtaaataa  <  1:1463278/70‑1 (MQ=255)                                             aCGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTtaaataa  <  1:752255/70‑1 (MQ=255)                                                       |                                                            TTAGCGGAGCCTGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTTAAATAA  >  W3110S.gb/2795751‑2795864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑