| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,821,480 | T→C | 63.1% | T316A (ACC→GCC) | recA ← | DNA strand exchange and recombination protein with protease and nuclease activity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,821,480 | 0 | T | C | 63.1% | 41.2 / 43.5 | 46 | T316A (ACC→GCC) | recA | DNA strand exchange and recombination protein with protease and nuclease activity |
| Reads supporting (aligned to +/- strand): ref base T (10/7); new base C (19/10); total (29/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTG > W3110S.gb/2821425‑2821544 | gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCttt > 1:1039627/1‑71 (MQ=255)gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCttt > 1:978342/1‑71 (MQ=255)gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCttt > 1:2987141/1‑71 (MQ=255)gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCttt > 1:1577903/1‑71 (MQ=255)gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCttt > 1:1330594/1‑71 (MQ=255)gAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCttt > 1:1287592/1‑71 (MQ=255) aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:1364159/71‑1 (MQ=255) aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:1454582/71‑1 (MQ=255) aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:2287161/71‑1 (MQ=255) aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:2211602/71‑1 (MQ=255) aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:1100036/71‑1 (MQ=255) aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:2184916/71‑1 (MQ=255) aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:2083292/71‑1 (MQ=255) aaCTCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGc < 1:1927906/71‑1 (MQ=255) cTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGTCAGTCGCATTCGCtt < 1:303743/71‑1 (MQ=255) cTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt < 1:1066299/71‑1 (MQ=255) cTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt < 1:2598976/71‑1 (MQ=255) cTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt < 1:2419334/71‑1 (MQ=255) taaCGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt > 1:1172733/3‑70 (MQ=255) tCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGt > 1:496021/1‑40 (MQ=255) tCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt > 1:1732896/1‑70 (MQ=255) tCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt > 1:692333/1‑70 (MQ=255) tCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt > 1:699724/1‑70 (MQ=255) tCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt > 1:3199502/1‑70 (MQ=255) tCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt > 1:1203616/1‑70 (MQ=255) tCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt > 1:818874/1‑70 (MQ=255) tCACGTACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt > 1:1029973/1‑70 (MQ=255) tCACGTACTTTCTTCTCGATCTCTTTAGCGGCTTCCGGGTTATCTTTCAGCCat > 1:3029216/1‑53 (MQ=38) tACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCt > 1:1540037/1‑71 (MQ=255) tACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCt > 1:438745/1‑71 (MQ=255) tACTTTCTTCTCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCt > 1:1987969/1‑71 (MQ=255) tcttctCGATCTCTTTCGCGGCTTCCGGGTTATCTTTc > 1:1460650/1‑38 (MQ=255) tcttctCGATCTCTTTCGCGGCTTCCGGGTTATCTTTc > 1:2701905/1‑38 (MQ=255) tcttctCGATCTCTTTCGCGGCTTCCGGGTTATCTTTc > 1:615863/1‑38 (MQ=255) tcttctCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGAcc > 1:969857/1‑70 (MQ=255) tcttctCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGAcc > 1:1543173/1‑70 (MQ=255) tcttctCGATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGAcc > 1:1643666/1‑70 (MQ=255) gATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtcttc < 1:3012978/70‑1 (MQ=255) gATCTCTTTCGCGGCTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGAtcttc < 1:2026057/70‑1 (MQ=255) aTCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa < 1:2826525/54‑1 (MQ=255) aTCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTa < 1:297783/54‑1 (MQ=255) tctcTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCtt < 1:1728297/51‑1 (MQ=255) tctTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGa > 1:1380953/1‑57 (MQ=255) tctTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGa > 1:1582750/1‑57 (MQ=255) tCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTg > 1:674795/1‑71 (MQ=255) tCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTg > 1:1107154/1‑71 (MQ=255) | GAGTTCGGGTTGCTCAGCAGCAACTCACGTACTTTCTTCTCGATCTCTTTCGCGGTTTCCGGGTTATCTTTCAGCCAGGCAGTCGCATTCGCTTTACCCTGACCGATCTTCTCACCTTTG > W3110S.gb/2821425‑2821544 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |