| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,866,110 | T→A | 100% | intergenic (‑20/+160) | rpoS ← / ← nlpD | RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,866,110 | 0 | T | A | 100.0% | 89.8 / NA | 29 | intergenic (‑20/+160) | rpoS/nlpD | RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (10/19); total (10/19) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
ACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTC > W3110S.gb/2866072‑2866171 | aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:3279754/1‑42 (MQ=255)aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:3187165/1‑42 (MQ=255)aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:1202466/1‑42 (MQ=255)aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:879555/1‑42 (MQ=255)aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:1655534/1‑42 (MQ=255)aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:1733003/1‑42 (MQ=255)aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:2092366/1‑42 (MQ=255)aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:823653/1‑42 (MQ=255)aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:277760/1‑42 (MQ=255)aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAAtt > 1:2498908/1‑42 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCg < 1:2442959/70‑1 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCg < 1:3154670/70‑1 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCg < 1:1161636/70‑1 (MQ=255) cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc < 1:2573599/71‑1 (MQ=255) cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc < 1:1002760/71‑1 (MQ=255) cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc < 1:799775/71‑1 (MQ=255) cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc < 1:3116012/71‑1 (MQ=255) cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc < 1:1651926/71‑1 (MQ=255) cTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc < 1:1100539/71‑1 (MQ=255) tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc < 1:2652143/52‑1 (MQ=255) tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc < 1:751378/52‑1 (MQ=255) tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc < 1:489962/52‑1 (MQ=255) tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc < 1:3306462/52‑1 (MQ=255) tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc < 1:3233956/52‑1 (MQ=255) tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc < 1:3195321/52‑1 (MQ=255) tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc < 1:2219710/52‑1 (MQ=255) tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc < 1:2122994/52‑1 (MQ=255) tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtctc < 1:1580494/52‑1 (MQ=255) tGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTc < 1:883641/70‑1 (MQ=255) | ACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTC > W3110S.gb/2866072‑2866171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |