| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,005,355 | G→A | 22.3% | K146K (AAG→AAA) | ygeW → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,005,355 | 0 | G | A | 22.3% | 172.0 / 44.1 | 85 | K146K (AAG→AAA) | ygeW | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (48/18); new base A (11/8); total (59/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTC > W3110S.gb/3005309‑3005423 | aCGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGgtg > 1:591967/1‑54 (MQ=255)aCGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTa > 1:1020514/1‑56 (MQ=255)aCGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTa > 1:1094081/1‑56 (MQ=255)aCGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTa > 1:2775825/1‑56 (MQ=255)aCGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTa > 1:2837694/1‑56 (MQ=255)aCGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTa > 1:1897058/1‑56 (MQ=255)aCGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTa > 1:1805116/1‑56 (MQ=255)aCGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTa > 1:3127750/1‑56 (MQ=255)aCGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTa > 1:949437/1‑56 (MQ=255)aCGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTa > 1:3134040/1‑56 (MQ=255)aCGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTa > 1:346738/1‑56 (MQ=255)aCGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTa > 1:571562/1‑56 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGc > 1:216598/1‑71 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGc > 1:2549067/1‑71 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGc > 1:3180945/1‑71 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGc > 1:1014968/1‑71 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGc > 1:1537441/1‑71 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGc > 1:1085140/1‑71 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGc > 1:381342/1‑71 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGc > 1:730640/1‑71 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGc > 1:2760531/1‑71 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGc > 1:1126347/1‑71 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGc > 1:97394/1‑71 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGc > 1:1311454/1‑71 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGc > 1:301911/1‑71 (MQ=255) gCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGc > 1:1244010/1‑71 (MQ=255) tatGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAg > 1:1530421/1‑71 (MQ=255) tatGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAg > 1:2055463/1‑71 (MQ=255) tatGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAg > 1:962137/1‑71 (MQ=255) tatGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAg > 1:1814957/1‑71 (MQ=255) tatGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAg > 1:579348/1‑71 (MQ=255) tGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAg > 1:1662424/1‑69 (MQ=255) tGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGt > 1:2967888/1‑70 (MQ=255) tGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGt < 1:871973/70‑1 (MQ=255) tGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGt > 1:861489/1‑70 (MQ=255) tGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGt > 1:3251476/1‑70 (MQ=255) tGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGt > 1:455551/1‑70 (MQ=255) tGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGt > 1:2480912/1‑70 (MQ=255) tGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGt > 1:1633517/1‑70 (MQ=255) tGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGt > 1:147025/1‑70 (MQ=255) tGCGTGAAGTTGGCGCTGCACTTGATCACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGt > 1:2121551/1‑70 (MQ=255) cGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGaa > 1:1926392/1‑71 (MQ=255) cGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGaa > 1:548244/1‑71 (MQ=255) cGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGaa > 1:1771645/1‑71 (MQ=255) cGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGaa > 1:2882009/1‑71 (MQ=255) cGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGaa > 1:1169902/1‑71 (MQ=255) cGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGaa > 1:1237926/1‑71 (MQ=255) cGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGaa > 1:2604732/1‑71 (MQ=255) aGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCtcc < 1:696383/71‑3 (MQ=255) aGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGc < 1:638900/71‑1 (MQ=255) aGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGc < 1:349816/71‑1 (MQ=255) aGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGc < 1:3211420/71‑1 (MQ=255) aGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGc < 1:1515071/71‑1 (MQ=255) aGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGc < 1:1685133/71‑1 (MQ=255) aGTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGc < 1:1456292/71‑1 (MQ=255) gTTGGCGCTGCACTTGATGACGGTTACAAACAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGc < 1:2076243/70‑1 (MQ=255) gcTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGa < 1:3236733/71‑1 (MQ=255) gcTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGa < 1:1545606/71‑1 (MQ=255) gcTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGa < 1:1333075/71‑1 (MQ=255) cTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCTGCttt < 1:1936452/52‑1 (MQ=255) cTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCttt < 1:927506/52‑1 (MQ=255) cTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCttt < 1:943564/52‑1 (MQ=255) cTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCttt < 1:2192067/52‑1 (MQ=255) cTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCttt < 1:88533/52‑1 (MQ=255) cTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCttt < 1:1760536/52‑1 (MQ=255) cTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATTCGa < 1:1736159/70‑1 (MQ=255) gCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAg > 1:2183365/1‑52 (MQ=255) gCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAg > 1:1514260/1‑52 (MQ=255) gCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAg > 1:971125/1‑52 (MQ=255) gCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAg > 1:2963110/1‑52 (MQ=255) gCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAg > 1:2112147/1‑52 (MQ=255) gCACTTAATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCgg > 1:430169/1‑46 (MQ=255) aCGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCAccc > 1:992577/1‑70 (MQ=255) aCGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCAccc > 1:1543066/1‑70 (MQ=255) ggTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCACCCGAc > 1:2143621/1‑71 (MQ=255) aCAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCaa > 1:811682/1‑47 (MQ=255) aCAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCaa > 1:76239/1‑47 (MQ=255) aCAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCaa > 1:153801/1‑47 (MQ=255) aaGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTc < 1:139873/71‑1 (MQ=255) aaGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTc < 1:2486192/71‑1 (MQ=255) aaGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTc < 1:241940/71‑1 (MQ=255) aaGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTc < 1:1316555/71‑1 (MQ=255) aaGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTc < 1:2242531/71‑1 (MQ=255) aaGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTc < 1:3134349/71‑1 (MQ=255) aaGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTc < 1:3210640/71‑1 (MQ=255) | ACGCCTATATGCGTGAAGTTGGCGCTGCACTTGATGACGGTTACAAGCAGGGTGTACTGCCACAGCGTCCGGCTTTAGTGAACCTGCAATGCGATATTGACCACCCGACTCAGTC > W3110S.gb/3005309‑3005423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |