| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,013,681 | G→A | 16.2% | V11V (GTC→GTT) | yqeC ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,013,681 | 0 | G | A | 16.2% | 88.2 / 10.0 | 37 | V11V (GTC→GTT) | yqeC | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (15/16); new base A (3/3); total (18/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCC > W3110S.gb/3013612‑3013747 | caagcTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGa > 1:225969/1‑71 (MQ=255) aacaacAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATAc > 1:294009/1‑71 (MQ=255) aacaacAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATAc > 1:15472/1‑71 (MQ=255) aacaacAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATAc > 1:2473323/1‑71 (MQ=255) aacaacAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATAc > 1:2029535/1‑71 (MQ=255) gAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCAc > 1:589131/1‑71 (MQ=255) caaaTCACAGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCAc > 1:2718742/2‑71 (MQ=255) cacaGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc > 1:1328107/1‑71 (MQ=255) cacaGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTc > 1:1909380/1‑71 (MQ=255) caGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:3348590/71‑1 (MQ=255) caGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:3144744/71‑1 (MQ=255) caGTGGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:35433/71‑1 (MQ=255) caGTCGGACGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:775497/71‑1 (MQ=255) aGTGGGCCGTTTCTGCGCACCTAAATCAATAACTAAAGCCGATGGGTCAACTATACTTTTCACGAGTCtt < 1:2011782/70‑1 (MQ=255) aCGTTTCTGCGCACCTAAATCAATAACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGAcc > 1:887198/1‑71 (MQ=255) tCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACctctct > 1:2897379/1‑71 (MQ=255) catcTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:1530059/68‑1 (MQ=255) cACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:237044/71‑1 (MQ=255) cACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:2491278/71‑1 (MQ=255) cACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:2505450/71‑1 (MQ=255) cACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:270436/71‑1 (MQ=255) cACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:589387/71‑1 (MQ=255) cACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:3105044/71‑1 (MQ=255) cACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:766249/71‑1 (MQ=255) cACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:40412/71‑1 (MQ=255) cACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:3302415/71‑1 (MQ=255) cACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:1995606/71‑1 (MQ=255) cACCTAAATCAATGACTAATGCCGATGGGACAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:1880188/71‑1 (MQ=255) aCCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:89114/70‑1 (MQ=255) aCCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATa < 1:1671688/70‑1 (MQ=255) aaaTCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAAtt > 1:2793700/1‑70 (MQ=255) aaaTCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTa > 1:3212384/1‑71 (MQ=255) aaaTCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTa > 1:2803040/1‑71 (MQ=255) aaaTCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTa > 1:2308897/1‑71 (MQ=255) aaTGACTAATGGCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATccc > 1:1481431/1‑70 (MQ=255) aaTGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATccc > 1:320995/1‑70 (MQ=255) aaTGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATccc > 1:1397238/1‑70 (MQ=255) | CAAGCTGGTTTTCCCCCCAGCACCAACAACAGAAATCACAGTGGGACGTTTCTGCGCACCTAAATCAATGACTAATGCCGATGGGTCAACTATACTTTTCACGAGTCTTTATGACCTCTCTGGGATAAATTATCCC > W3110S.gb/3013612‑3013747 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |