| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,135,166 | A→G | 49.3% | intergenic (‑139/+153) | pitB ← / ← gss | phosphate transporter/fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,135,166 | 0 | A | G | 49.3% | ‑5.5 / 111.1 | 76 | intergenic (‑139/+153) | pitB/gss | phosphate transporter/fused glutathionylspermidine amidase and glutathionylspermidine synthetase |
| Reads supporting (aligned to +/- strand): ref base A (4/34); new base G (24/13); total (29/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.03e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.64e-01 | |||||||||||
AAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAA > W3110S.gb/3135099‑3135232 | aaaTATCATTTTTTTACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAc < 1:2515379/70‑1 (MQ=255)aaaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAcc < 1:2182000/71‑1 (MQ=255)aaaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAcc < 1:2315618/71‑1 (MQ=255)aaaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAcc < 1:2757137/71‑1 (MQ=255)aaaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAcc < 1:1178353/71‑1 (MQ=255)aaaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAcc < 1:558138/71‑1 (MQ=255)aaaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAc < 1:1676672/70‑1 (MQ=255)aaaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAc < 1:2707975/70‑1 (MQ=255)aaaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAc < 1:3206412/70‑1 (MQ=255)aaaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAc < 1:3283750/70‑1 (MQ=255)aaaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAc < 1:601354/70‑1 (MQ=255)aaaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAc < 1:1082166/70‑1 (MQ=255)aaaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCAATCAAAAc < 1:1550270/70‑1 (MQ=255) aaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAc < 1:1044934/69‑1 (MQ=255) aaTATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAAc < 1:756256/69‑1 (MQ=255) tttttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGaa < 1:2948245/69‑1 (MQ=255) tttttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGaa < 1:730061/69‑1 (MQ=255) tttttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGaa < 1:1787793/69‑1 (MQ=255) tttttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGaa < 1:1921329/69‑1 (MQ=255) tttttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGaa < 1:2442945/69‑1 (MQ=255) tttttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGaa < 1:406952/69‑1 (MQ=255) ttttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACATTTAGAaat > 1:1882078/1‑70 (MQ=255) tttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat > 1:2721295/1‑69 (MQ=255) tttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat > 1:950131/1‑69 (MQ=255) tttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat > 1:2648123/1‑69 (MQ=255) tttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat > 1:790156/1‑69 (MQ=255) tttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat > 1:2504402/1‑69 (MQ=255) tttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat > 1:68751/1‑69 (MQ=255) tttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat > 1:1432162/1‑69 (MQ=255) tttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat > 1:1856827/1‑69 (MQ=255) tttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat > 1:1991326/1‑69 (MQ=255) tttAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAaat > 1:1109501/1‑69 (MQ=255) tttAACGATAAAAAGAGTGTTATTTCTTGTGTAACAGACAATAAGCATTCATTCAAGACCTTTAGAaat > 1:2061453/1‑69 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCaaaa < 1:304946/71‑1 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCaaaa < 1:272980/71‑1 (MQ=255) gATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATT‑‑AAACCTTTAGAAATAATCAAAaca < 1:879409/71‑1 (MQ=255) tAAAAATAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAaca < 1:126247/71‑1 (MQ=255) tAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAaca < 1:2014861/71‑1 (MQ=255) tAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAaca < 1:668775/71‑1 (MQ=255) tAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAaca < 1:24386/71‑1 (MQ=255) tAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAaca < 1:1713610/71‑1 (MQ=255) tAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAaca < 1:868406/71‑1 (MQ=255) tAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAaca < 1:3234979/71‑1 (MQ=255) tAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAaca < 1:1686424/71‑1 (MQ=255) tAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAaca < 1:3016100/71‑1 (MQ=255) tAAAAAGACTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAaca < 1:2642818/71‑1 (MQ=255) aaaaGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAacac < 1:2065798/70‑1 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt > 1:542694/1‑71 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt > 1:140232/1‑71 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt > 1:333783/1‑71 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt > 1:80436/1‑71 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt > 1:1046115/1‑71 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACt > 1:25285/1‑71 (MQ=255) aTTTCTTGTGTATCAGACAATAAGCATTCATTCAACACCTTTAGAAATAATCAAAACACAGGTATTCCAc > 1:2663423/1‑70 (MQ=255) tATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAatata > 1:2084791/1‑71 (MQ=255) tATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAatata > 1:3303031/1‑71 (MQ=255) aCAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa < 1:2202473/61‑1 (MQ=255) aCAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa < 1:1134451/61‑1 (MQ=255) aCAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa < 1:1250307/61‑1 (MQ=255) aCAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa < 1:683021/61‑1 (MQ=255) aCAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa < 1:3020221/61‑1 (MQ=255) aCAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa < 1:2783627/61‑1 (MQ=255) cAATAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTaa < 1:2513911/60‑1 (MQ=255) cAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTcaca > 1:2161541/1‑70 (MQ=255) tAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATTTATTCACatat > 1:1872333/1‑70 (MQ=255) tAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTcac > 1:3245468/1‑66 (MQ=255) tAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACatat > 1:397715/1‑70 (MQ=255) tAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACatat > 1:885337/1‑70 (MQ=255) tAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACatat > 1:2887240/1‑70 (MQ=255) tAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACatat > 1:1601477/1‑70 (MQ=255) tAAGCATTCATTCAAGACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACatat > 1:2174277/1‑70 (MQ=255) caAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGaataaa < 1:1254417/70‑1 (MQ=255) caAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGaataaa < 1:1094170/70‑1 (MQ=255) caAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGaataaa < 1:2050959/70‑1 (MQ=255) caAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGaataaa < 1:1972256/70‑1 (MQ=255) caAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGaataaa < 1:3128866/70‑1 (MQ=255) | AAATATCATTTTTTAACGATAAAAAGAGTGTTATTTCTTGTGTATCAGACAATAAGCATTCATTCAAAACCTTTAGAAATAATCAAAACACAGGTATTCCACTGGTTAATATATTCACATATGAAATGAATAAA > W3110S.gb/3135099‑3135232 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |