| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,304,430 | A→G | 24.2% | *415Q (TAA→CAA) | mtr ← | tryptophan transporter of high affinity |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,304,430 | 0 | A | G | 24.2% | 165.4 / 56.0 | 91 | *415Q (TAA→CAA) | mtr | tryptophan transporter of high affinity |
| Reads supporting (aligned to +/- strand): ref base A (29/40); new base G (12/10); total (41/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGA > W3110S.gb/3304362‑3304486 | gACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt < 1:3146991/71‑1 (MQ=255)gACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt < 1:60693/71‑1 (MQ=255)gACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt < 1:1843484/71‑1 (MQ=255)gACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt < 1:184276/71‑1 (MQ=255)gACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt < 1:615782/71‑1 (MQ=255)gACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt < 1:1759237/71‑1 (MQ=255)gACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt < 1:670658/71‑1 (MQ=255)gACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt < 1:2210216/71‑1 (MQ=255)gACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt < 1:221178/71‑1 (MQ=255)gACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt < 1:3081364/71‑1 (MQ=255)gACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt < 1:231527/71‑1 (MQ=255)gACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt < 1:1105050/71‑1 (MQ=255) aCCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACt < 1:3340363/70‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCTCTGATTACTGATa < 1:1517921/69‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:2257296/69‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:2108136/69‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:2093779/69‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:2035293/69‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:449225/69‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:474342/69‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:2654136/69‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:276043/69‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:445207/69‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:1614043/69‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:891886/69‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:1489/69‑1 (MQ=255) aTAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:672208/69‑1 (MQ=255) aTAAGCAAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATa < 1:1218249/69‑1 (MQ=255) aagcgaagcgCATCAGGCATTGTGTAGGCAGGAGAAATGTCGGATAAGGCACCGCTGATTACTGATaca > 1:667890/1‑69 (MQ=255) aagcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATacac > 1:2596622/1‑70 (MQ=255) aagcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATaca > 1:1940388/1‑69 (MQ=255) aagcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATaca > 1:2960104/1‑69 (MQ=255) aagcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATaca > 1:3329221/1‑69 (MQ=255) aagcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATaca > 1:1082784/1‑69 (MQ=255) aagcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATaca > 1:212834/1‑69 (MQ=255) aagcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATaca > 1:2919069/1‑69 (MQ=255) aagcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACAGCTGATTACTGATaca > 1:2969724/1‑69 (MQ=255) agcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACAcc > 1:2203862/1‑70 (MQ=255) agcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACAcc > 1:2752005/1‑70 (MQ=255) agcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCg > 1:2921644/1‑71 (MQ=255) agcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCg > 1:1647953/1‑71 (MQ=255) agcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCg > 1:334121/1‑71 (MQ=255) agcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCg > 1:1189545/1‑71 (MQ=255) agcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCg > 1:100035/1‑71 (MQ=255) agcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCg > 1:3066017/1‑71 (MQ=255) agcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCg > 1:2453289/1‑71 (MQ=255) agcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCg > 1:2413751/1‑71 (MQ=255) agcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCg > 1:441850/1‑71 (MQ=255) agcgaagcgCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCg > 1:2387334/1‑71 (MQ=255) cATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATacac > 1:245449/1‑60 (MQ=255) cATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAAt > 1:3074744/1‑71 (MQ=255) cATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAAt > 1:2769399/1‑71 (MQ=255) cATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAAt > 1:1578555/1‑71 (MQ=255) gtgtAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGa > 1:2511764/1‑70 (MQ=255) gtgtAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGa > 1:1352781/1‑70 (MQ=255) gtgtAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGa > 1:3101613/1‑70 (MQ=255) ggCAGCTGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCGGCAGTAAATTAAAGCTCGATaaaa < 1:1040110/70‑1 (MQ=255) ggCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCGGCAGTAAATTAAAGCTCGATaaaa < 1:2801210/70‑1 (MQ=255) ggCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCGGCAGTAAATTAAAGCTCGATaaaa < 1:2794422/70‑1 (MQ=255) ggCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCGGCAGTAAATTAAAGCTCGATaaaa < 1:3349323/70‑1 (MQ=255) ggCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCGGCAGTAAATTAAAGCTCGATaaaa < 1:2605398/70‑1 (MQ=255) ggCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCGGCAGTAAATTAAAGCTCGATaaaa < 1:2593376/70‑1 (MQ=255) ggCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCGGCAGTAAATTAAAGCTCGATaaaa < 1:2345155/70‑1 (MQ=255) ggCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCGGCAGTAAATTAAAGCTCGATaaaa < 1:2244957/70‑1 (MQ=255) ggCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCGGCAGTAAATTAAAGCTCGATaaaa < 1:1456399/70‑1 (MQ=255) ggCAGCAGAAATGTCGGATAAGGCACCGCTGATTGCTGATACACCGGCAGTAAATTAAAGCTCGATaaaa < 1:758176/70‑1 (MQ=255) gcagcaGAAATGTCGGATAAGGCACCGCTGATTACTGATa > 1:419574/1‑40 (MQ=255) gcagcaGAAATGTCGGATAAGGCACCGCTGATTACTGATa > 1:2590246/1‑40 (MQ=255) gcagcaGAAATGTCGGATAAGGCACCGCTGATTACTGATa > 1:1090556/1‑40 (MQ=255) gcagcaGAAATGTCGGATAAGGCACCGCTGATTACTGATa > 1:2852807/1‑40 (MQ=255) gcagcaGAAATGTCGGATAAGGCACCGCTGATTACTGATa > 1:2570923/1‑40 (MQ=255) gcagcaGAAATGTCGGATAAGGCACCGCTGATTACTGATa > 1:3163009/1‑40 (MQ=255) gcagcaGAAATGTCGGATAAGGCACCGCTGATTACTGATa > 1:1598794/1‑40 (MQ=255) gcagcaGAAATGTCGGATAAGGCACCGCTGATTACTGATa > 1:1856445/1‑40 (MQ=255) gcagAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGTCAGTAAATTAAAGCTCGATaaaa < 1:1819519/66‑1 (MQ=255) cagAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGc > 1:3003685/1‑70 (MQ=255) cagAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGc > 1:763168/1‑70 (MQ=255) cagAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGc > 1:2404671/1‑70 (MQ=255) cagAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGc > 1:537999/1‑70 (MQ=255) aTAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATaaaa < 1:1226506/53‑1 (MQ=255) ccGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGcc < 1:1927203/64‑1 (MQ=255) ccGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGcc < 1:985403/64‑1 (MQ=255) ccGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGcc < 1:1371291/64‑1 (MQ=255) ccGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGcc < 1:1544541/64‑1 (MQ=255) ccGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGcc < 1:498965/64‑1 (MQ=255) ccGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGcc < 1:461720/64‑1 (MQ=255) ccGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGcc < 1:1974048/64‑1 (MQ=255) ccGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGcc < 1:311731/64‑1 (MQ=255) ccGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGcc < 1:3313650/64‑1 (MQ=255) gCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGcc < 1:2102220/62‑1 (MQ=255) gCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGa > 1:2629445/1‑64 (MQ=255) | GACCTGATAAGCGAAGCGCATCAGGCATTGTGTAGGCAGCAGAAATGTCGGATAAGGCACCGCTGATTACTGATACACCGGCAGTAAATTAAAGCTCGATAAAATATGCACCAGTGCGTTGCCGA > W3110S.gb/3304362‑3304486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |