| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,363,632 | T→C | 40.0% | F324S (TTT→TCT) | yhcD → | predicted outer membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,363,632 | 0 | T | C | 40.0% | 15.0 / 31.4 | 30 | F324S (TTT→TCT) | yhcD | predicted outer membrane protein |
| Reads supporting (aligned to +/- strand): ref base T (3/15); new base C (5/7); total (8/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.10e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.05e-01 | |||||||||||
GTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGAT > W3110S.gb/3363563‑3363690 | gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:2623280/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:996396/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:917515/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:660766/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:424908/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:3173365/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:3169406/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:3160551/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:2962549/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:2566248/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:2555788/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:2418226/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:2280487/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:1969771/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:1950176/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:1861912/1‑70 (MQ=255)gTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGtt > 1:1815138/1‑70 (MQ=255) ccGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTCTAATCAGCATGTAGaa < 1:2021143/71‑1 (MQ=255) ccGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTCTAATCAGCATGTAGaa < 1:2223214/71‑1 (MQ=255) ccGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTCTAATCAGCATGTAGaa < 1:1652012/71‑1 (MQ=255) ccGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTCTAATCAGCATGTAGaa < 1:1180067/71‑1 (MQ=255) ccGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTCTAATCAGCATGTAGaa < 1:2711028/71‑1 (MQ=255) ccGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTCTAATCAGCATGTAGaa < 1:2811300/71‑1 (MQ=255) cGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTCTAATCAGCATGTAGaa < 1:2851614/70‑1 (MQ=255) gTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTCTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGc > 1:1603076/1‑71 (MQ=255) gTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTCTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGc > 1:1700107/1‑71 (MQ=255) gTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTCTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGc > 1:252232/1‑71 (MQ=255) gTAGTTCGCACAACTATATTGTTCCCTCCACACTGTCTAATCAGCATGt > 1:42488/1‑49 (MQ=255) gTAGTTCGCACAACTATATTGTTCCCTACACCCTGTCTAATCAGCATGTAGAAAGCTTCCAGGGTTATCg > 1:756024/1‑70 (MQ=255) cGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTcgc > 1:660433/1‑70 (MQ=255) cGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTcgc > 1:1728640/1‑70 (MQ=255) cGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTcgc > 1:3007342/1‑70 (MQ=255) ttCCCTCCACCGTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGAc < 1:2887055/71‑1 (MQ=255) ttCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGAc < 1:903943/71‑1 (MQ=255) ttCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGAc < 1:1545253/71‑1 (MQ=255) ttCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGAc < 1:970767/71‑1 (MQ=255) ttCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGAc < 1:1037006/71‑1 (MQ=255) ttCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGAc < 1:3293914/71‑1 (MQ=255) ttCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGAc < 1:3170734/71‑1 (MQ=255) ttCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGAc < 1:3110862/71‑1 (MQ=255) ttCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGAc < 1:1021062/71‑1 (MQ=255) ttCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGAc < 1:2242938/71‑1 (MQ=255) ccTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGAt < 1:2907917/71‑1 (MQ=255) ccTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGAt < 1:1529750/71‑1 (MQ=255) ccTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGAt < 1:2715936/71‑1 (MQ=255) ccTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGAt < 1:1951734/71‑1 (MQ=255) ccTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGAt < 1:1984049/71‑1 (MQ=255) | GTGATCTTAATGTCACCGTTGTCGAAACAGACGGTAGTTCGCACAACTATATTGTTCCCTCCACCCTGTTTAATCAGCATGTAGAAAGCTTCCAGGGTTATCGCTTCGCGATAGGGCGGGTAGACGAT > W3110S.gb/3363563‑3363690 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |