| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,575,604 | C→T | 8.8% | A211T (GCC→ACC) | yihL ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,575,604 | 0 | C | T | 8.8% | 168.7 / 5.7 | 57 | A211T (GCC→ACC) | yihL | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (40/12); new base T (3/2); total (43/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.06e-01 | |||||||||||
GCCTCAAATCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATACCCGCAGAACCGCCAAGATACTTGCTGATATCGC > W3110S.gb/3575549‑3575669 | gCCTCAAATCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCaa > 1:2803348/1‑70 (MQ=255)gCCTCAAATCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAAt > 1:2404606/1‑71 (MQ=255)gCCTCAAATCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAAt > 1:2181795/1‑71 (MQ=255)gCCTCAAATCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAAt > 1:219712/1‑71 (MQ=255)gCCTCAAATCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAAt > 1:1621164/1‑71 (MQ=255)gCCTCAAATCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAAt > 1:3159106/1‑71 (MQ=255)gCCTCAAATCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAAt > 1:2357743/1‑71 (MQ=255)gCCTCAAATCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAAt > 1:1398769/1‑71 (MQ=255)gCCTCAAATCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAAt > 1:1010937/1‑71 (MQ=255)gCCTCAAATCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGAGATGCTTTTCAAt > 1:2732484/1‑71 (MQ=255)gCCTCAAATCGCCAATATTCAATATCTATTTGGACAATAATGCCTTGCTGGTCGGCGCGATGCTTTTCAAt > 1:2820312/1‑71 (MQ=255) aaaTCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAAtaag < 1:1179802/69‑4 (MQ=255) aaaTCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAg < 1:302401/70‑1 (MQ=255) aaaTCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAg < 1:95894/70‑1 (MQ=255) aaaTCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAg < 1:3222184/70‑1 (MQ=255) aaaTCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAg < 1:1700953/70‑1 (MQ=255) aaaTCGCCAATATTCAATATCTATTTGGACAATATTGCCTAGCTGGTCGGCGCGATGCTTTTCAATTAAg < 1:2177386/70‑1 (MQ=255) cAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTgg < 1:1983612/71‑1 (MQ=255) cAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTgg < 1:2711416/71‑1 (MQ=255) cAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTgg < 1:635708/71‑1 (MQ=255) cAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTgg < 1:2465175/71‑1 (MQ=255) cAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTgg < 1:1384141/71‑1 (MQ=255) cAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTgg < 1:1827871/71‑1 (MQ=255) ttCAATATCTATTTGGACATTATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:1366803/1‑71 (MQ=255) ttCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:2490643/1‑71 (MQ=255) ttCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:2949254/1‑71 (MQ=255) ttCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:1336525/1‑71 (MQ=255) ttCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:2596929/1‑71 (MQ=255) ttCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:3010699/1‑71 (MQ=255) ttCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:2544283/1‑71 (MQ=255) ttCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:3550/1‑71 (MQ=255) ttCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:3289133/1‑71 (MQ=255) ttCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:1748351/1‑71 (MQ=255) ttCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:1545607/1‑71 (MQ=255) ttCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:2254711/1‑71 (MQ=255) ttCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:1603537/1‑71 (MQ=255) ttCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:612086/1‑71 (MQ=255) ttCAATATCTAGTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATAc > 1:2979377/1‑71 (MQ=255) tCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGa > 1:110904/1‑36 (MQ=255) tCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGa > 1:3149365/1‑36 (MQ=255) tCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGa > 1:818921/1‑36 (MQ=255) tCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGa > 1:2780838/1‑36 (MQ=255) tCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGa > 1:255966/1‑36 (MQ=255) tCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGa > 1:2465931/1‑36 (MQ=255) tCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGa > 1:2232594/1‑36 (MQ=255) tCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGa > 1:1703588/1‑36 (MQ=255) tCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGa > 1:1679140/1‑36 (MQ=255) tCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGa > 1:1368819/1‑36 (MQ=255) tCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGa > 1:1180467/1‑36 (MQ=255) gCCTTGCTGGTCGGTGCGATGCTTTTCAATTAAGATCGCTGGCATACCCGCAGAACCGCCAAGATACTTGc > 1:2412809/1‑71 (MQ=255) gCCTTGCTGGTCGGTGCGATGCTTTTCAATTAAGATCGCTGGCATACCCGCAGAACCGCCAAGATACTTGc > 1:372238/1‑71 (MQ=255) gCCTTGCTGGTCGGTGCGATGCTTTTCAATTAAGATCGCTGGCATACCCGCAGAACCGCCAAGATACTTGc > 1:715494/1‑71 (MQ=255) gCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATACCCGCAGAACCGCCAAGATACTTGc > 1:2208362/1‑71 (MQ=255) gCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATACCCGCAGAACCGCCAAGATACTTGc > 1:583192/1‑71 (MQ=255) gCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATACCCGCAGAACCGCCAAGATACTTGc > 1:1280218/1‑71 (MQ=255) gTCGGTGCGATGCTTTTCAATTAAGATCGCTGGCATACCCGCAGAACCGCCAAGATACTTGCTGATATCGc < 1:2905941/71‑1 (MQ=255) gTCGGTGCGATGCTTTTCAATTAAGATCGCTGGCATACCCGCAGAACCGCCAAGATACTTGCTGATATCGc < 1:1983758/71‑1 (MQ=255) | GCCTCAAATCGCCAATATTCAATATCTATTTGGACAATATTGCCTTGCTGGTCGGCGCGATGCTTTTCAATTAAGATCGCTGGCATACCCGCAGAACCGCCAAGATACTTGCTGATATCGC > W3110S.gb/3575549‑3575669 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |