| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,587,198 | C→T | 29.6% | E840K (GAA→AAA) | polA ← | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,587,198 | 0 | C | T | 29.6% | 76.3 / 39.9 | 54 | E840K (GAA→AAA) | polA | fused DNA polymerase I 5'‑>3' exonuclease, 3'‑>5' polymerase and 3'‑>5' exonuclease |
| Reads supporting (aligned to +/- strand): ref base C (16/22); new base T (13/3); total (29/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.54e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.69e-01 | |||||||||||
CGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACAGACGG > W3110S.gb/3587143‑3587253 | cGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCacgacg < 1:2655107/71‑1 (MQ=255)cGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCacgacg < 1:3165804/71‑1 (MQ=255)cGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCacgacg < 1:148984/71‑1 (MQ=255) aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCAt > 1:96731/1‑71 (MQ=255) aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCAt > 1:2420021/1‑71 (MQ=255) aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCAt > 1:2388737/1‑71 (MQ=255) aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCAt > 1:2812382/1‑71 (MQ=255) aaTGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACCTTTAGCCGCTGCACGACGAGCACCAt > 1:1878073/1‑71 (MQ=255) tCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCt > 1:1713566/1‑71 (MQ=255) gcggTTCCCTGCATTGGCGCGTTAATGGCTGCATGTTTAGCCGCTGCACGACGAGCACCATTGCTGGAttt < 1:3331224/71‑1 (MQ=255) cggTTCCCTGCATTGGCGCGTTAATGGCTGCATGTTTAGCCGCTGCACGACGAGCACCATTGCTGGAttt < 1:3254718/70‑1 (MQ=255) cggTTCCCTGCATTGGCGCGTTAATGGCTGCATGTTTAGCCGCTGCACGACGAGCACCATTGCTGGAttt < 1:3106709/70‑1 (MQ=255) gTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGat < 1:3078920/71‑1 (MQ=255) gTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGat < 1:1374536/71‑1 (MQ=255) gTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGat < 1:371677/71‑1 (MQ=255) gTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGat < 1:835900/71‑1 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:986708/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:3354573/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:2980757/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:2974520/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:2695523/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:2689880/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:261498/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:389216/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:2493317/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:2449954/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:2283367/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:2159046/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:1353648/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:2139533/1‑70 (MQ=255) gCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCgg > 1:1475278/1‑70 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGata > 1:48861/1‑60 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGata > 1:512304/1‑60 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGata > 1:556275/1‑60 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGata > 1:827062/1‑60 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGata > 1:1691730/1‑60 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGata > 1:894478/1‑60 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCTGCACGACGAGCACCATTGCTGGATTTGata > 1:2114523/1‑60 (MQ=255) ggCGCGTTAATGGCTGCACGTTTAGCCGCAGCacgacg > 1:1986342/1‑38 (MQ=37) cgcgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTTGATTTGATATCCGGCAGATACa < 1:1194502/71‑1 (MQ=255) cgcgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:939067/71‑1 (MQ=255) cgcgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:1111391/71‑1 (MQ=255) cgcgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:2317816/71‑1 (MQ=255) cgcgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:1424557/71‑1 (MQ=255) cgcgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:1471950/71‑1 (MQ=255) cgcgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:3063704/71‑1 (MQ=255) cgcgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:214721/71‑1 (MQ=255) cgcgTTAATGGCGGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:1986372/71‑1 (MQ=255) gcgTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACa < 1:67593/70‑1 (MQ=255) aaTGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACAGACgg < 1:1350500/70‑1 (MQ=255) aaTGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACAGACgg < 1:539913/70‑1 (MQ=255) aaTGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACAGACgg < 1:1666412/70‑1 (MQ=255) aaTGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACAGACgg < 1:1010906/70‑1 (MQ=255) aTGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACAGACgg < 1:2948281/69‑1 (MQ=255) | CGTTTGATAATGTCGGCGGCGGTTCCCTGCATTGGCGCGTTAATGGCTGCACGTTCAGCCGCTGCACGACGAGCACCATTGCTGGATTTGATATCCGGCAGATACAGACGG > W3110S.gb/3587143‑3587253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |