Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	3,682,573	G→A	100%	L211L (CTG→TTG)	ilvD ←	dihydroxyacid dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,682,573	0	G	A	87.5%	15.3 / ‑3.3	8	L211L (CTG→TTG)	ilvD	dihydroxyacid dehydratase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (0/7);  total (1/7)
Fisher's exact test for biased strand distribution p-value = 1.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.25e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

GGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGG  >  W3110S.gb/3682526‑3682623                                                |                                                   ggCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTg                             <  1:266147/71‑1 (MQ=255) ggCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTg                             <  1:2687433/71‑1 (MQ=255) ggCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTg                             <  1:2948144/71‑1 (MQ=255) ggCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTg                             <  1:3346384/71‑1 (MQ=255)       ggTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAg                       >  1:2459001/1‑71 (MQ=255)                             cccGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAgg  <  1:1209712/70‑1 (MQ=255)                             cccGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAgg  <  1:1636409/70‑1 (MQ=255)                             cccGGCTGCGACAGGCCCAACGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAgg  <  1:21604/70‑1 (MQ=255)                                                |                                                   GGCGTGGGTTGCCAGCAGCGAGCCGTTGCCCGGCTGCGACAGGCCCAGCGCTTCGGTCAGGCAGTTCATTGAGTTAGCGGTAAACATCCCGGAGCAGG  >  W3110S.gb/3682526‑3682623

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑