| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,701,087 | A→G | 100% | A103A (GCT→GCC) | rbsC ← | D‑ribose transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,701,087 | 0 | A | G | 78.3% | 42.1 / 8.0 | 23 | A103A (GCT→GCC) | rbsC | D‑ribose transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base G (18/0); total (23/0) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01 | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTGCGCCGGTCAGCGCC > W3110S.gb/3701038‑3701156 | cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:2413786/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:61834/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:3314813/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:3218271/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:291153/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:2803528/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:2765680/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:256980/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:2407201/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:2373502/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:209019/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:2010968/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:1965692/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:1824329/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:1558327/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:1381651/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:1264256/1‑71 (MQ=255)cTACAATCACCCCAGTTACCGCACCAATTGCAGCACCTAACGCGAGAGCGGCAGCGACAGCCACCAGCGCa > 1:1264507/1‑71 (MQ=255) tACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCaa > 1:1101727/1‑65 (MQ=255) tACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATg > 1:2728393/1‑67 (MQ=255) tACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATg > 1:2825738/1‑67 (MQ=255) tACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATg > 1:1310256/1‑67 (MQ=255) tACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATg > 1:601516/1‑67 (MQ=255) cagcagcGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTGCGCCGGTCAGCGcc < 1:2923525/71‑1 (MQ=255) | CTACAATCACCCCGGTTACCGCACCAATTGCGGCACCTAACGCGAGAGCAGCAGCGACAGCCACCAGCGCATTGACTTCAATGCCGACGATAGATGCAGCAACTGCGCCGGTCAGCGCC > W3110S.gb/3701038‑3701156 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |