Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | W3110S.gb | 1,682,197 | Δ1 bp | 33.3% | coding (927/1383 nt) | ydgI → | predicted arginine/ornithine antiporter transporter |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | W3110S.gb | 1,682,197 | 0 | C | . | 33.3% | 65.0 / 27.7 | 30 | coding (927/1383 nt) | ydgI | predicted arginine/ornithine antiporter transporter |
Reads supporting (aligned to +/- strand): ref base C (9/11); new base . (6/4); total (15/15) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 7.00e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01 |
TTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCC > W3110S.gb/1682134‑1682263 | tttGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTc < 1:3310528/64‑1 (MQ=255) gATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGgccgcc < 1:2031826/70‑1 (MQ=255) cGTTTCCGTTTGCGGCGAGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACt > 1:459089/1‑70 (MQ=255) tGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTT‑CTGCCCGCCACTCATAAAGCATTc > 1:3715364/1‑71 (MQ=255) cTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCa > 1:1795961/1‑58 (MQ=255) ttAAGCTGGACCATCATGGCAGCAGAAGTACCGTT‑CTGGCCGCCACTCATa < 1:2683680/51‑1 (MQ=255) tAAGCTGGACCATCATGGCAGCAGAAGTACCGTT‑CTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTg > 1:2587525/1‑71 (MQ=255) atcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAg < 1:2712014/69‑1 (MQ=255) tcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATa < 1:1342398/39‑1 (MQ=255) tcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATa < 1:166424/39‑1 (MQ=255) tcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATa < 1:3654815/39‑1 (MQ=255) tcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAg < 1:1533069/68‑1 (MQ=255) tcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAg < 1:1655464/68‑1 (MQ=255) tcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAg < 1:394121/68‑1 (MQ=255) atGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACg > 1:2069097/1‑70 (MQ=255) atGGCAGCAGAAGTACCGTT‑CTGGCCGCCACTCATAAAg < 1:253516/39‑1 (MQ=38) tGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGc < 1:538933/70‑1 (MQ=255) tGGCAGCAGAAGTACCGTT‑CTGTCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGaa > 1:964238/1‑66 (MQ=255) tGGCAGCAGAAGTACCGTT‑CTGGCCGCCACTCATAAAGTATTCCCGCGCATTTTTGCGCGCCAGaa > 1:631466/1‑66 (MQ=255) tGGCAGCAGAAGTACCGTT‑CTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGaa > 1:2314970/1‑66 (MQ=255) ggCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCa > 1:143854/1‑40 (MQ=255) gcagcaGAAGTACCGTT‑CTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGcaca < 1:1659099/70‑1 (MQ=255) gcagcaGAAGTACCGTT‑CTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGcaca < 1:807716/70‑1 (MQ=255) aGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAg < 1:2251032/55‑1 (MQ=255) aGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTgcgc > 1:1004747/1‑71 (MQ=255) gTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTgcgc > 1:2559592/1‑70 (MQ=255) gTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTgcgc > 1:2573230/1‑70 (MQ=255) gTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTgcgc > 1:2432814/1‑70 (MQ=255) tACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCAttttt < 1:2220071/44‑1 (MQ=255) ccGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATc > 1:2176941/1‑71 (MQ=255) ccGTT‑CTGGCCGCCACTCATAAAGCATTCCAGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATcc > 1:3013575/1‑71 (MQ=255) | TTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCC > W3110S.gb/1682134‑1682263 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |