Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 1,682,197 Δ1 bp 33.3% coding (927/1383 nt) ydgI → predicted arginine/ornithine antiporter transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb1,682,1970C.33.3% 65.0 / 27.7 30coding (927/1383 nt)ydgIpredicted arginine/ornithine antiporter transporter
Reads supporting (aligned to +/- strand):  ref base C (9/11);  new base . (6/4);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 7.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.87e-01

TTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCC  >  W3110S.gb/1682134‑1682263
                                                               |                                                                  
tttGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTc                                                                    <  1:3310528/64‑1 (MQ=255)
   gATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGgccgcc                                                           <  1:2031826/70‑1 (MQ=255)
      cGTTTCCGTTTGCGGCGAGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACt                                                        >  1:459089/1‑70 (MQ=255)
                tGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCTGCCCGCCACTCATAAAGCATTc                                            >  1:3715364/1‑71 (MQ=255)
                           cTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCa                                               >  1:1795961/1‑58 (MQ=255)
                            ttAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCTGGCCGCCACTCATa                                                    <  1:2683680/51‑1 (MQ=255)
                             tAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTg                               >  1:2587525/1‑71 (MQ=255)
                                        atcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAg                       <  1:2712014/69‑1 (MQ=255)
                                         tcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATa                                                    <  1:1342398/39‑1 (MQ=255)
                                         tcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATa                                                    <  1:166424/39‑1 (MQ=255)
                                         tcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATa                                                    <  1:3654815/39‑1 (MQ=255)
                                         tcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAg                       <  1:1533069/68‑1 (MQ=255)
                                         tcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAg                       <  1:1655464/68‑1 (MQ=255)
                                         tcatGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAg                       <  1:394121/68‑1 (MQ=255)
                                           atGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACg                   >  1:2069097/1‑70 (MQ=255)
                                           atGGCAGCAGAAGTACCGTTCTGGCCGCCACTCATAAAg                                                 <  1:253516/39‑1 (MQ=38)
                                            tGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGc                  <  1:538933/70‑1 (MQ=255)
                                            tGGCAGCAGAAGTACCGTTCTGTCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGaa                     >  1:964238/1‑66 (MQ=255)
                                            tGGCAGCAGAAGTACCGTTCTGGCCGCCACTCATAAAGTATTCCCGCGCATTTTTGCGCGCCAGaa                     >  1:631466/1‑66 (MQ=255)
                                            tGGCAGCAGAAGTACCGTTCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGaa                     >  1:2314970/1‑66 (MQ=255)
                                             ggCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCa                                               >  1:143854/1‑40 (MQ=255)
                                              gcagcaGAAGTACCGTTCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGcaca               <  1:1659099/70‑1 (MQ=255)
                                              gcagcaGAAGTACCGTTCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGcaca               <  1:807716/70‑1 (MQ=255)
                                                      aGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAg                       <  1:2251032/55‑1 (MQ=255)
                                                      aGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTgcgc       >  1:1004747/1‑71 (MQ=255)
                                                       gTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTgcgc       >  1:2559592/1‑70 (MQ=255)
                                                       gTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTgcgc       >  1:2573230/1‑70 (MQ=255)
                                                       gTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTgcgc       >  1:2432814/1‑70 (MQ=255)
                                                        tACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCAttttt                                <  1:2220071/44‑1 (MQ=255)
                                                          ccGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATc   >  1:2176941/1‑71 (MQ=255)
                                                          ccGTTCTGGCCGCCACTCATAAAGCATTCCAGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATcc  >  1:3013575/1‑71 (MQ=255)
                                                               |                                                                  
TTTGATCGTTTCCGTTTGCGGCGCGTACTTAAGCTGGACCATCATGGCAGCAGAAGTACCGTTCCTGGCCGCCACTCATAAAGCATTCCCGCGCATTTTTGCGCGCCAGAACGCACAAGCTGCGCCATCC  >  W3110S.gb/1682134‑1682263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: