| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 905,152 | Δ1 bp | 10.9% | coding (138/324 nt) | ybjQ → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 905,152 | 0 | T | . | 10.9% | 114.1 / 11.9 | 46 | coding (138/324 nt) | ybjQ | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (20/21); new base . (3/2); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAA > W3110S.gb/905082‑905206 | gCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGt > 1:3431039/1‑71 (MQ=255) aaGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTcc < 1:168129/71‑1 (MQ=255) ttttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg < 1:2879723/71‑1 (MQ=255) ttttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg < 1:1421223/71‑1 (MQ=255) ttttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg < 1:3710712/71‑1 (MQ=255) tttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg > 1:1488688/1‑70 (MQ=255) tttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg > 1:3799902/1‑70 (MQ=255) tttAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg < 1:683291/70‑1 (MQ=255) tttAGGTGCCAATATTTTCCGTGATTTCTTTCCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCg > 1:3527298/1‑70 (MQ=255) ggTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATg < 1:3908055/70‑1 (MQ=255) ggTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGa < 1:1238570/71‑1 (MQ=255) gCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTa > 1:2667592/1‑64 (MQ=255) gCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTa > 1:281983/1‑64 (MQ=255) gCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACG‑TCCGGTGCGTa > 1:695164/1‑64 (MQ=255) tatTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTcc < 1:3793189/52‑1 (MQ=255) tatTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTcc < 1:1341507/52‑1 (MQ=255) atTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGtt > 1:572034/1‑49 (MQ=255) atTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGAGGACGtt > 1:3337325/1‑49 (MQ=255) ttttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTg > 1:3034553/1‑71 (MQ=255) ttttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTg > 1:485761/1‑71 (MQ=255) ttttCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTg > 1:2857674/1‑71 (MQ=255) cGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTa < 1:3459039/70‑1 (MQ=255) cGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTa < 1:1693055/70‑1 (MQ=255) aTTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAg < 1:151622/57‑1 (MQ=255) cTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACgg < 1:1779480/70‑1 (MQ=255) ccGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGgag < 1:2940660/68‑1 (MQ=255) ggTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCAc < 1:3627414/61‑1 (MQ=255) ggTATCCGAGATATCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAg < 1:2259880/45‑1 (MQ=38) tCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAg < 1:3309324/42‑1 (MQ=255) tCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGgag < 1:3640317/62‑1 (MQ=255) tCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGgag < 1:2097124/62‑1 (MQ=255) tCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCtt < 1:3867008/70‑1 (MQ=255) cgcgATATCGTTGGCGGACGTTCCTGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCttt > 1:564233/1‑69 (MQ=255) cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGAt > 1:2048707/1‑62 (MQ=255) cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGAt > 1:3644304/1‑62 (MQ=255) cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCttt > 1:3164587/1‑69 (MQ=255) cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCttt > 1:2968876/1‑69 (MQ=255) cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCttt > 1:3554048/1‑69 (MQ=255) cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCttt > 1:1488524/1‑69 (MQ=255) cgcgATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCttt > 1:2382120/1‑69 (MQ=255) cgcgATATCGTTGGCGGACCTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGgaga > 1:2316229/1‑61 (MQ=255) gATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAgg < 1:1621506/70‑1 (MQ=255) atatCGTTGGCGGACG‑TCCGGTGCGTATGAAAAAg < 1:166459/35‑1 (MQ=37) tCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGgag < 1:162062/53‑1 (MQ=255) tCGTAGGCGGACGTTCCGGTGCGTATGAAAAAGaa > 1:2947988/1‑35 (MQ=255) gTTGGCGGCCGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGaa > 1:2179410/1‑66 (MQ=255) gTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTaaa > 1:1025370/1‑42 (MQ=255) gTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGaa > 1:1060524/1‑66 (MQ=255) gTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGaa > 1:1718409/1‑66 (MQ=255) ttCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAgg < 1:316204/53‑1 (MQ=255) ttCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAgg < 1:2892888/53‑1 (MQ=255) ttCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAgg < 1:1582091/53‑1 (MQ=255) | GCGAAGCGATTTTAGGTGCCAATATTTTCCGTGATTTCTTTGCCGGTATCCGCGATATCGTTGGCGGACGTTCCGGTGCGTATGAAAAAGAACTGCGTAAAGCACGGGAGATCGCCTTTGAGGAA > W3110S.gb/905082‑905206 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |