| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,042,843 | Δ1 bp | 27.1% | coding (1790/2181 nt) | yccC ← | cryptic autophosphorylating protein tyrosine kinase Etk |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,042,843 | 0 | A | . | 27.1% | 138.5 / 68.0 | 70 | coding (1790/2181 nt) | yccC | cryptic autophosphorylating protein tyrosine kinase Etk |
| Reads supporting (aligned to +/- strand): ref base A (24/27); new base . (9/10); total (33/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.40e-01 | |||||||||||
AGGTGGCACCTGACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACAGGTTATGC > W3110S.gb/1042776‑1042908 | aGGTCGCACCTGACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATTCTGGATCACTTTGTTGAGCt > 1:3439818/1‑71 (MQ=255) ggCACCTGACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCa > 1:966075/1‑69 (MQ=255) ggCACCTGACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCa > 1:2716353/1‑69 (MQ=255) ggCACCTGACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTT‑AGCTCa > 1:286413/1‑68 (MQ=255) aCCTGACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCttt < 1:1053059/71‑1 (MQ=255) aCCTGACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCttt < 1:3920588/71‑1 (MQ=255) aCCTGACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGAGCACTTTGTTGAGCTCATCttt < 1:1286081/71‑1 (MQ=255) ccTGACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTa < 1:981947/71‑1 (MQ=255) ccTGACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTa < 1:3096458/71‑1 (MQ=255) cTGACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTAc < 1:3399350/71‑1 (MQ=255) gACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCt > 1:914491/1‑71 (MQ=255) gACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCt > 1:499502/1‑71 (MQ=255) gAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGa < 1:1947091/71‑1 (MQ=255) gAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGa < 1:2722688/71‑1 (MQ=255) gAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGa < 1:82973/71‑1 (MQ=255) gTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGatat < 1:3125190/71‑1 (MQ=255) gTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGatat < 1:3660945/71‑1 (MQ=255) gTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGatat < 1:1991312/71‑1 (MQ=255) tAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGatat < 1:2795972/70‑1 (MQ=255) aTCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTAcc > 1:1342106/1‑59 (MQ=255) aTCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTAcc > 1:4020292/1‑59 (MQ=255) cacaTCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCttt < 1:3412537/54‑1 (MQ=255) acaTCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCAt > 1:2114898/1‑48 (MQ=255) caTCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGAc < 1:2380354/71‑1 (MQ=255) caTCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGAc < 1:92927/71‑1 (MQ=255) aTCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGATATTCCGAc > 1:2215518/1‑69 (MQ=255) aTCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGATATTCCGAc > 1:2254242/1‑69 (MQ=255) aTCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGATATTCCGAc > 1:2642230/1‑69 (MQ=255) tCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCttt < 1:513459/50‑1 (MQ=255) cAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGata > 1:2162331/1‑61 (MQ=255) aaaGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTAc > 1:2745596/1‑50 (MQ=255) aaaGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTAc > 1:1337732/1‑50 (MQ=255) aaaGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTAc > 1:369448/1‑50 (MQ=255) aaaGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTAc > 1:3030298/1‑50 (MQ=255) aaaGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATAtt > 1:2365273/1‑62 (MQ=255) aaaGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCATATATTCCGACAAGc > 1:795978/1‑70 (MQ=255) aaaGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGATATTCCGACAAGc > 1:1846449/1‑70 (MQ=255) aaGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGATATTCCGACAAGcc < 1:102329/70‑1 (MQ=255) gCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGc > 1:3823651/1‑37 (MQ=255) cctcctTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGc < 1:1168230/67‑1 (MQ=255) cctcctTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGc < 1:3198013/67‑1 (MQ=255) cctcctTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGc < 1:3259338/67‑1 (MQ=255) ctTTGCCAAAATGCTGGATCACTTTGTTTAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGtt < 1:1000520/69‑1 (MQ=255) ctTTGCCAAAATGCTGGATCACTTTGTTTAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGtt < 1:1965601/69‑1 (MQ=255) ctTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTc < 1:896657/70‑1 (MQ=255) ctTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTc < 1:1215307/70‑1 (MQ=255) ctTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCa > 1:2442445/1‑71 (MQ=255) ctTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCAt < 1:35050/71‑1 (MQ=255) ctTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCAt < 1:3517142/71‑1 (MQ=255) ctTTGCCAAAATGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCAt < 1:1315371/71‑1 (MQ=255) tttGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGtt < 1:3371763/68‑1 (MQ=255) tGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATg > 1:2610306/1‑64 (MQ=255) tGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCa > 1:3619538/1‑68 (MQ=255) tGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCa > 1:2237462/1‑68 (MQ=255) tGCTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTa < 1:743574/71‑1 (MQ=255) cTGGATCACTTTGTTG‑GCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTaaa < 1:661472/71‑1 (MQ=255) cACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACTAGCCATGTTc < 1:3991696/51‑1 (MQ=255) cACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACAGGt > 1:1397478/1‑71 (MQ=255) cACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACAGGt > 1:2667291/1‑71 (MQ=255) cACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACAGGt > 1:1141130/1‑71 (MQ=255) cACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACAGGt > 1:3312377/1‑71 (MQ=255) aCTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACAGGt < 1:4033739/70‑1 (MQ=255) aCTTTGTTG‑GCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGt > 1:195171/1‑47 (MQ=255) tttGTTGAGCTCATCTTTACCTTCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACa < 1:1526664/65‑1 (MQ=255) tttGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACa < 1:409449/65‑1 (MQ=255) tttGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACa < 1:2811125/65‑1 (MQ=255) tttGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACa < 1:2190442/65‑1 (MQ=255) ttgttg‑GCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACAGGTTATGc > 1:1335311/1‑71 (MQ=255) ttgttg‑GCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACAGGTTATGc > 1:3355944/1‑71 (MQ=255) tgttgAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACAGGTTATGc > 1:3167571/1‑71 (MQ=255) | AGGTGGCACCTGACCGCGAGTAATCACATCAAAGCCTCCTTTGCCAAAATGCTGGATCACTTTGTTGAGCTCATCTTTACCTGCCAGATATTCCGACAAGCCATGTTCATTACTCACGGTAAACAGGTTATGC > W3110S.gb/1042776‑1042908 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |