| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,701,562:1 | +C | 28.9% | coding (494/1593 nt) | malX → | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,701,562 | 1 | . | C | 28.9% | 50.2 / 49.8 | 38 | coding (494/1593 nt) | malX | fused maltose and glucose‑specific PTS enzyme IIBC components |
| Reads supporting (aligned to +/- strand): ref base . (5/22); new base C (4/7); total (9/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
GTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAATT > W3110S.gb/1701494‑1701623 | gTCGATCGATACCGGGTTCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cc < 1:375731/71‑1 (MQ=255)gTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cc < 1:114868/71‑1 (MQ=255) tcgatcgaTACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCa < 1:691533/71‑1 (MQ=255) gatcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cccat < 1:3189147/71‑4 (MQ=255) gatcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cccat < 1:1866546/71‑4 (MQ=255) gatcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cccat < 1:1741184/71‑4 (MQ=255) gatcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cccat < 1:1774363/71‑4 (MQ=255) gatcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑cccat < 1:307637/71‑4 (MQ=255) gatcgatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCata < 1:3392466/71‑1 (MQ=255) agatACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCataata > 1:1533367/2‑71 (MQ=255) gATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTTCCCATAATATCCGCCTg < 1:2124215/71‑1 (MQ=255) gATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTTCCCATAATATCCGCCTg < 1:2181304/71‑1 (MQ=255) gATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTTCCCATAATATCCGCCTg < 1:207021/71‑1 (MQ=255) tCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTTCCCATAATATcc < 1:2307717/64‑1 (MQ=255) tCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCg < 1:2780041/71‑1 (MQ=255) tCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCg < 1:3168083/71‑1 (MQ=255) tCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCg < 1:1161616/71‑1 (MQ=255) ggtgATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTgg < 1:1237063/71‑1 (MQ=255) tgATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCat < 1:608009/44‑1 (MQ=255) tgATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCTCTGGCa < 1:521789/71‑1 (MQ=255) gATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTTCCCATAatat > 1:3968160/1‑49 (MQ=39) gATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTTCCCATAatat > 1:3330645/1‑49 (MQ=39) aTCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCg < 1:2266413/57‑1 (MQ=255) aTCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCg < 1:416592/57‑1 (MQ=255) attatCGTCTGGATGCTGCATGAGCTTTTCCCataat < 1:2292596/37‑1 (MQ=25) attatCGTCTGGATGCTGCATGAGCGTTTCCCataat < 1:1475198/37‑1 (MQ=37) ttatCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCAt < 1:609882/60‑1 (MQ=255) ttatCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTcggcgg < 1:2363559/70‑1 (MQ=255) atCGTCTGGATGCTGCATGAGCGTTTCCCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTcgg > 1:2461851/1‑66 (MQ=255) atCGTCTGGATGCTGCATGAGCGTTTCCCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTcgg > 1:1172078/1‑66 (MQ=255) atCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGa > 1:1790589/1‑47 (MQ=255) atCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTAc > 1:3798595/1‑71 (MQ=255) tCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTAcg > 1:3298985/1‑71 (MQ=255) tCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTAcg > 1:2553804/1‑71 (MQ=255) tgcATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCAt < 1:141194/45‑1 (MQ=255) tgcATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCAt < 1:2717378/45‑1 (MQ=255) cATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCa < 1:2027429/69‑1 (MQ=255) tGAGCGTTTCCCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAAtt < 1:1274208/71‑1 (MQ=255) tGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCAttc < 1:209012/43‑1 (MQ=255) tGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCAttc < 1:1847423/43‑1 (MQ=255) tGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCtt < 1:2720150/60‑1 (MQ=255) tGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCtt < 1:2613372/60‑1 (MQ=255) tGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAAtt < 1:717167/70‑1 (MQ=255) tGAGCGTTT‑CCATAATATCCGCCTGCCAGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAAtt < 1:1356962/70‑1 (MQ=255) gAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAAtt < 1:2316627/69‑1 (MQ=255) ttt‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTcggcgg < 1:1829524/45‑1 (MQ=255) | GTCGATCGATACCGGGATCCTCGGTGCGGTGATCGCCGGTATTATCGTCTGGATGCTGCATGAGCGTTT‑CCATAATATCCGCCTGCCGGATGCGCTGGCATTCTTCGGCGGTACGCGCTTCGTACCAATT > W3110S.gb/1701494‑1701623 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |