| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,641,490:1 | +G | 40.7% | coding (113/1425 nt) | creC → | sensory histidine kinase in two‑component regulatory system with CreB or PhoB, regulator of the CreBC regulon |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,641,490 | 1 | . | G | 40.7% | ‑3.6 / 51.9 | 27 | coding (113/1425 nt) | creC | sensory histidine kinase in two‑component regulatory system with CreB or PhoB, regulator of the CreBC regulon |
| Reads supporting (aligned to +/- strand): ref base . (13/3); new base G (3/8); total (16/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTG > W3110S.gb/4641428‑4641555 | ggcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGa > 1:1533217/1‑71 (MQ=255)ggcAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGa > 1:3377045/1‑71 (MQ=255) cTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGa > 1:3588057/1‑60 (MQ=255) gTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATc < 1:128026/70‑1 (MQ=255) tCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGa > 1:3819083/1‑70 (MQ=255) cTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTGATCGaca > 1:4074718/1‑68 (MQ=255) cTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATc > 1:3237603/1‑63 (MQ=255) gCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGacac > 1:1311115/1‑65 (MQ=255) gCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCaa < 1:3591925/70‑1 (MQ=255) ccATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGGATGGGACGTTGATCGACACCGCAAc < 1:3820502/71‑1 (MQ=255) cATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGacac > 1:1274482/1‑63 (MQ=255) cATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGt < 1:2003258/71‑1 (MQ=255) aTTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTGATCGACACCGCAACGt < 1:2631186/71‑1 (MQ=255) aTTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTGATCGACACCGCAACGt < 1:3958926/71‑1 (MQ=255) tttttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTGATCGACACCGCAACGtt < 1:3250910/71‑1 (MQ=255) tttttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTGATCGACACCGCAACGtt < 1:3538638/71‑1 (MQ=255) tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAg > 1:1206718/1‑42 (MQ=255) tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCt > 1:2697373/1‑71 (MQ=255) tttGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCt > 1:3154250/1‑71 (MQ=255) tAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGgcg > 1:2626561/1‑71 (MQ=255) tAAACCGGGCGTGCGAAGAGCAACGGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGgcg > 1:1504940/1‑71 (MQ=255) cGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGAtt > 1:2586410/1‑71 (MQ=255) cGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGAtt > 1:2084811/1‑71 (MQ=255) aaGAGCAACGGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGct < 1:3896003/70‑1 (MQ=255) gagCAACGGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGctctc < 1:3346890/71‑1 (MQ=255) agCAACGGGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGct < 1:3185380/67‑1 (MQ=255) agCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTg > 1:406042/1‑71 (MQ=255) | GGCAGCCTGGTTCGTACTGGCCATTTTTGTCAAAGAAGTTAAACCGGGCGTGCGAAGAGCAAC‑GGAGGGGACGTTGATCGACACCGCAACGTTGCTGGCGGAGCTGGCGCGTCCCGATTTGCTCTCTG > W3110S.gb/4641428‑4641555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |