| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 547,831:1 | +G | 100% | pseudogene (251/261 nt) pseudogene (251/1259 nt) |
ylbE → ylbE → |
ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 547,831 | 1 | . | G | 100.0% | 159.7 / NA | 48 | pseudogene (251/261 nt) pseudogene (251/1259 nt) | ylbE ylbE | ECK0512:JW0507:b4507; hypothetical protein, N‑ter fragment ECK0512:JW0508+JW0507:b4572; hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (29/19); total (29/19) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
GCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTT > W3110S.gb/547771‑547895 | gcgcATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTg < 1:1889767/71‑1 (MQ=255) aTGTCTGTTCGAATGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑ggggga < 1:1026824/63‑3 (MQ=255) tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt < 1:2916959/71‑1 (MQ=255) tGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACtt > 1:4050690/1‑71 (MQ=255) tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTc < 1:2681623/70‑1 (MQ=255) tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTc < 1:362591/70‑1 (MQ=255) tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCa > 1:1644524/1‑71 (MQ=255) tCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑g > 1:496654/1‑55 (MQ=255) tGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCAt > 1:1530588/1‑70 (MQ=255) ttCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCa > 1:2276753/1‑67 (MQ=255) cGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCt > 1:2146489/1‑70 (MQ=255) gTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAc < 1:3706382/71‑1 (MQ=255) ttGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAcc < 1:3365364/71‑1 (MQ=255) ttGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCAc > 1:1345045/1‑70 (MQ=255) tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCa > 1:3626417/1‑71 (MQ=255) tGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCa > 1:3056327/1‑71 (MQ=255) gggCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCa < 1:1052265/70‑1 (MQ=255) cGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:3835874/71‑1 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt > 1:2433357/1‑71 (MQ=255) aaGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAAt > 1:928564/1‑71 (MQ=255) aGATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATg > 1:2579763/1‑71 (MQ=255) gATGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGc < 1:2719072/71‑1 (MQ=255) aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc < 1:160240/71‑1 (MQ=255) aTGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGcc < 1:3556361/71‑1 (MQ=255) tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGc > 1:1020238/1‑69 (MQ=255) tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg > 1:115634/1‑71 (MQ=255) tGAAGCGCAGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCg > 1:3066216/1‑71 (MQ=255) aGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:952368/56‑1 (MQ=255) aGGCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAtgtg < 1:1350298/56‑1 (MQ=255) tgcgcTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa < 1:2744679/70‑1 (MQ=255) ggCGCTGGCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCaa < 1:1916613/71‑1 (MQ=255) gCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg < 1:234740/71‑1 (MQ=255) gCAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTAGTCACCATGTGAATGCCGTCGGGcc < 1:154237/62‑1 (MQ=255) cAATACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCgg > 1:2791582/1‑70 (MQ=255) aaTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCAt > 1:718151/1‑43 (MQ=38) aTACTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTCAATGCCGTCGGGcc < 1:3292033/59‑1 (MQ=255) aCTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt > 1:2811722/1‑51 (MQ=255) aCTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGt > 1:1935752/1‑51 (MQ=255) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:2137508/1‑44 (MQ=38) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:322538/1‑44 (MQ=38) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGaa > 1:705431/1‑44 (MQ=38) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc > 1:813744/1‑71 (MQ=255) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc > 1:2836595/1‑71 (MQ=255) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc < 1:3472854/71‑1 (MQ=255) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTAc > 1:2942133/1‑71 (MQ=255) cTGGAGCAGGGGGAAGTGAACTTCATTCCTTGACACCATGTGAAt > 1:574122/1‑45 (MQ=255) ggAGCAGGGGGAAGTGAACTTCATTCGTTGTCACCATGTGAATGCCGTCGGGCCAAt > 1:228001/1‑57 (MQ=255) ggAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAAt > 1:2741878/1‑57 (MQ=255) ggAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACtt > 1:2618544/1‑71 (MQ=255) ggAGCAGGGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCAGGCCAATGGGCGGTATTACtt > 1:1063894/1‑71 (MQ=255) | GCGCATGTCTGTTCGAAGGTTGGGCGAAAGATGAAGCGCAGGCGCTGGCAATACTGGAGCA‑GGGGAAGTGAACTTCATTCCTTGTCACCATGTGAATGCCGTCGGGCCAATGGGCGGTATTACTT > W3110S.gb/547771‑547895 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |