Predicted mutation
evidence seq id position mutation freq annotation gene description
MC JC W3110S.gb 654,214 Δ1,199 bp 100% [dcuC][dcuC] [dcuC], insH, [dcuC]

Missing coverage evidence...
   seq id start end size ←reads reads→ gene description
* * ÷ W3110S.gb 654214–655411 655412 2–1199 37 [0] [0] 33 [dcuC]–[dcuC] [dcuC],insH,[dcuC]

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? W3110S.gb = 6542130 (0.000)24 (0.640) 16/90 NT 100% coding (70/477 nt) dcuC anaerobic C4‑dicarboxylate transport
?W3110S.gb 655413 = 0 (0.000)coding (978/1092 nt) dcuC anaerobic C4‑dicarboxylate transport

CATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/654139‑654213
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC  >  W3110S.gb/655413‑655480
                                                                                                                                               
CATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGC                                                                          <  1:3793312/71‑1
 ATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                         <  1:493569/71‑1
 ATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCT                                                                         <  1:2779147/71‑1
    AGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCTATGGTGCTAA                                                                       <  1:1082385/70‑1
    AGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG                                                                      >  1:1222392/1‑71
    AGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG                                                                      >  1:3439909/1‑71
    AGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG                                                                      >  1:3165641/1‑71
    AGGATGATACTCGCCGAACAAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG                                                                      >  1:558273/1‑71
       ATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCC                                                                   >  1:1460449/1‑71
           TACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCT                                                                 >  1:399486/1‑69
            ACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAG                                                              >  1:1735271/1‑71
            ACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAG                                                              >  1:3844057/1‑71
             CTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGC                                                             >  1:1249842/1‑71
               CGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                            >  1:750819/1‑70
               CGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTA                                                                        >  1:3243154/1‑58
               CGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTA                                                                        >  1:149199/1‑58
                GCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAA                                                          >  1:2210619/1‑71
                GCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAA                                                          >  1:3630598/1‑71
                GCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                            <  1:2831129/69‑1
                GCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                            <  1:3798757/69‑1
                           CGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGC                                                                     >  1:3000254/1‑49
                           CGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGC                                                                     >  1:369896/1‑49
                                 AGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATA                                                        >  1:584993/1‑56
                                    GATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCA                                      <  1:1743585/71‑1
                                        GAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCAT                                  >  1:3131290/1‑71
                                        GAAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCAT                                  >  1:3212502/1‑71
                                         AAATCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGC                                    <  1:355678/68‑1
                                            TCAGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAA                                                          <  1:69598/43‑1
                                              AGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCAC                            >  1:1593995/1‑71
                                              AGACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCAC                            >  1:3794675/1‑71
                                                ACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGT                          <  1:121575/71‑1
                                                ACTTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAG                                     <  1:534600/60‑1
                                                  TTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGT                          >  1:368227/1‑69
                                                  TTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGC                                              >  1:74247/1‑49
                                                  TTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                            <  1:3596518/35‑1
                                                  TTTGAATAAAGCCGATGGTGCTAAGCCCCTGAGCG                                                            <  1:2877910/35‑1
                                                    TGAATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCA                                         >  1:2844033/1‑52
                                                      AATAAAGCCGATGGTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCA                     <  1:3309352/70‑1
                                                                   GTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCAT       <  1:954577/71‑1
                                                                   GTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCAT       <  1:1770813/71‑1
                                                                   GTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCAT       <  1:1000934/71‑1
                                                                   GTGCTAAGCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGC          <  1:1934045/68‑1
                                                                        AAGCCCCTGAGCGAATACCCCAGCGGCACCCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC  >  1:87334/1‑71
                                                                                                                                               
CATCAGGATGATACTCGCCGAACCAAACGAGGTAGCGATAGAAATCAGACTTTGAATAAAGCCGATGGTGCTAAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  W3110S.gb/654139‑654213
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ctaagCCCCTGAGCGAATACCCCAGCGGCAACCAGCAGCATCACCACGTTAGCAAACGCATCTGCCATCCCGC  >  W3110S.gb/655413‑655480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.