| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 4,850 | T→G | 29.2% | F373V (TTC→GTC) | thrC → | threonine synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 4,850 | 0 | T | G | 29.2% | 30.0 / 46.2 | 48 | F373V (TTC→GTC) | thrC | threonine synthase |
| Reads supporting (aligned to +/- strand): ref base T (11/23); new base G (9/5); total (20/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.69e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.51e-01 | |||||||||||
AGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGA > W3110S.gb/4785‑4917 | aGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTc < 1:990050/71‑1 (MQ=255)aGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTc < 1:935055/71‑1 (MQ=255) cTGCCGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGTCTTGTTCCTCGGCACcgc > 1:1549858/1‑70 (MQ=255) cTGCCGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACcgcg > 1:1060371/1‑71 (MQ=255) aGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCgg > 1:1282711/1‑71 (MQ=255) aGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCgg > 1:2967250/1‑71 (MQ=255) ttATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGt < 1:1871836/47‑1 (MQ=255) tATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGa > 1:420818/1‑70 (MQ=255) tATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGa > 1:1840474/1‑70 (MQ=255) tGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTa < 1:2335654/71‑1 (MQ=255) tGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTa < 1:4037542/71‑1 (MQ=255) tGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTa < 1:3108761/71‑1 (MQ=255) tGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTa < 1:2761947/71‑1 (MQ=255) cGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAagagag < 1:1665963/71‑1 (MQ=255) tGATCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTa < 1:3697698/62‑1 (MQ=255) aTCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAAt > 1:1749709/1‑57 (MQ=255) aTCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAAt > 1:1087461/1‑57 (MQ=255) tCAGTTGAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTg < 1:757688/70‑1 (MQ=255) cAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTgg > 1:720142/1‑70 (MQ=255) ttGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTa < 1:1991433/55‑1 (MQ=255) ttGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGc < 1:835373/71‑1 (MQ=255) gAATCCAGGCGAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCg < 1:518996/70‑1 (MQ=255) aaTCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGa < 1:3587540/70‑1 (MQ=255) tCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCa < 1:2253212/36‑1 (MQ=255) tCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAAt < 1:73735/47‑1 (MQ=255) tCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATtc < 1:3518844/71‑1 (MQ=255) cAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGa < 1:2882420/42‑1 (MQ=255) gAATATGGCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGc > 1:513233/1‑59 (MQ=255) gAATATCGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTg > 1:520583/1‑70 (MQ=255) tatGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTaaa < 1:3127919/42‑1 (MQ=255) tatGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAagag < 1:1059480/45‑1 (MQ=255) tatGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAagag < 1:2691058/45‑1 (MQ=255) tGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAagaga > 1:3489586/1‑44 (MQ=255) tGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAagaga > 1:3800955/1‑44 (MQ=255) tGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAagaga > 1:1549215/1‑44 (MQ=255) tGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAagaga > 1:2379553/1‑44 (MQ=255) tGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAagaga > 1:1564184/1‑44 (MQ=255) tGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGt < 1:1501947/71‑1 (MQ=255) tGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGt < 1:3069942/71‑1 (MQ=255) ggCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGtt > 1:3567600/1‑71 (MQ=255) gCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAaga < 1:3641108/40‑1 (MQ=255) gCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAaga < 1:3456058/40‑1 (MQ=255) gCTTGGTGCTCGGCACCGCGCATCCGGCGAAATTTAAaga > 1:2624451/1‑40 (MQ=38) gCTTGGTCCTCGGCACCGCGCATCCGGCGAAATTTAAaga > 1:2912496/1‑40 (MQ=255) ttGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAagaga > 1:1067369/1‑40 (MQ=255) ttGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGcca < 1:756444/52‑3 (MQ=255) ttGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTgg < 1:130761/70‑1 (MQ=255) gTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGt < 1:2041563/65‑1 (MQ=255) gTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGa < 1:3949103/69‑1 (MQ=255) | AGCCGCACGCTGCCGTAGCTTATCGTGCGCTGCGTGATCAGTTGAATCCAGGCGAATATGGCTTGTTCCTCGGCACCGCGCATCCGGCGAAATTTAAAGAGAGCGTGGAAGCGATTCTCGGTGAAACGTTGGA > W3110S.gb/4785‑4917 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |