| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,545,084 | C→T | 16.0% | G231R (GGG→AGG) | narU ← | nitrate/nitrite transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,545,084 | 0 | C | T | 16.0% | 126.3 / 15.2 | 50 | G231R (GGG→AGG) | narU | nitrate/nitrite transporter |
| Reads supporting (aligned to +/- strand): ref base C (27/15); new base T (4/4); total (31/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01 | |||||||||||
TGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACAT > W3110S.gb/1545017‑1545153 | tgaaGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCt > 1:2185358/4‑69 (MQ=255)tGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGa > 1:2878616/1‑71 (MQ=255)tGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATT‑AGGCGCGTGAACTGGCGATATCATTCATCCCTGAc > 1:255660/1‑71 (MQ=255) gTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATgcggc > 1:155390/1‑70 (MQ=255) tAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATgcggc > 1:3633215/1‑69 (MQ=255) tAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGa > 1:2210766/1‑71 (MQ=255) ggAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCa < 1:77099/71‑1 (MQ=255) ggAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCa < 1:3681899/71‑1 (MQ=255) ggAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCa < 1:1465587/71‑1 (MQ=255) ggAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGAGATCGTGGCa < 1:3090998/71‑1 (MQ=255) cGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAg > 1:1487071/1‑70 (MQ=255) ggCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCTTGACCATGCGGCGATCGTGGCAATCGCCAg > 1:3926261/1‑69 (MQ=255) ggCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCTTGACCATGCGGCGATCGTGGCAATCGCCAg > 1:1117059/1‑69 (MQ=255) ggCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCTTGACCATGCGGCGATCGTGGCAATCGCCAg > 1:2215110/1‑69 (MQ=255) aTTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGa < 1:3327757/49‑1 (MQ=255) aTTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGa < 1:2883444/49‑1 (MQ=255) aTTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCgg < 1:2597682/71‑1 (MQ=255) ttGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGcc < 1:630505/62‑1 (MQ=255) ttGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGcc < 1:767901/62‑1 (MQ=255) ttGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGcc < 1:2678121/62‑1 (MQ=255) tGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGt > 1:3614366/1‑51 (MQ=255) gAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCa > 1:2158103/1‑38 (MQ=255) gAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCa > 1:3140064/1‑38 (MQ=255) gAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCa > 1:837029/1‑38 (MQ=255) gAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTAc > 1:3267169/1‑71 (MQ=255) gAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTAc > 1:1660580/1‑71 (MQ=255) cgcgTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCaa < 1:2442874/71‑1 (MQ=255) cgcgTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCaa < 1:740059/71‑1 (MQ=255) gTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAAt < 1:865845/70‑1 (MQ=255) gTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATc < 1:2038550/71‑1 (MQ=255) gCGATATCATCCCTCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCgg < 1:1650403/70‑1 (MQ=255) tatCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCAt > 1:3092203/1‑62 (MQ=255) atCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACGCAAATCCa > 1:1638543/1‑60 (MQ=255) atCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCa > 1:1169573/1‑60 (MQ=255) atCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCa > 1:1235901/1‑60 (MQ=255) atCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCa > 1:3719151/1‑60 (MQ=255) atCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCg > 1:1056493/1‑71 (MQ=255) atCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCg > 1:2206991/1‑71 (MQ=255) atCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCg > 1:1232582/1‑71 (MQ=255) tcattcatCCTTGACCATGCGGCGATCTTGGCAATCGCCAGTAGc < 1:1957249/45‑1 (MQ=38) tcattcatCCTTGACCATGCGGCGATCGTGGCAATCGCCAGTAGc < 1:1254986/45‑1 (MQ=255) tcattcatCCTTGACCATGCGGCGATCGTGGCAATCGCCAGTAGc < 1:1491675/45‑1 (MQ=255) tcattcatCCTTGACCATGCGGCGATCGTGGCAATCGCCAGTAGc < 1:189136/45‑1 (MQ=255) tcattcatCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTa > 1:1054380/1‑49 (MQ=255) tcattcatCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCa > 1:3431927/1‑66 (MQ=255) tcattcatCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCa > 1:2867111/1‑66 (MQ=255) tcattcatCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCa > 1:2704696/1‑66 (MQ=255) tcattcatCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCAtt > 1:3495266/1‑68 (MQ=255) tcattcatCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCAtt > 1:2052922/1‑68 (MQ=255) attcatCCTTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCgg > 1:1961692/1‑45 (MQ=255) ccTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACAt > 1:2607149/1‑71 (MQ=255) | TGCAGGCGTTGTAAGACAGGGAGCTGGTCGGCAATTGAGGCGCGTGAACTGGCGATATCATTCATCCCTGACCATGCGGCGATCGTGGCAATCGCCAGTAGCGGTACCCAAATCCATGCGGCATTCGCCAGCGACAT > W3110S.gb/1545017‑1545153 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |