| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,557,824 | C→T | 21.1% | Q57Q (CAG→CAA) | bdm ← | biofilm‑dependent modulation protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,557,824 | 0 | C | T | 21.1% | 153.8 / 34.7 | 71 | Q57Q (CAG→CAA) | bdm | biofilm‑dependent modulation protein |
| Reads supporting (aligned to +/- strand): ref base C (33/23); new base T (7/8); total (40/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.59e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.44e-01 | |||||||||||
AGGTTGCCGATCAAGCATAGCACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGT > W3110S.gb/1557755‑1557891 | aGGTTGCCGATCAAGCATAGCACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGc < 1:2671609/70‑1 (MQ=255) tGCCGATCAAGCATAGCACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTgg > 1:3258143/1‑69 (MQ=255) gCCGATCAAGCATAGCACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGTTGGTa < 1:1910777/70‑1 (MQ=255) cAAGCATAGCACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTg > 1:1152783/1‑61 (MQ=255) cAAGCATAGCACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCa > 1:1852490/1‑71 (MQ=255) cAAGCATAGCACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCa > 1:1134231/1‑71 (MQ=255) cAAGCATAGCACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCa > 1:1156577/1‑71 (MQ=255) gCATAGCACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTg < 1:613526/71‑1 (MQ=255) gCACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGAt < 1:70496/71‑1 (MQ=255) gCACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGAt > 1:2309015/1‑71 (MQ=255) gCACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGAt > 1:2946861/1‑71 (MQ=255) cACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGTTGGTAGa < 1:1849611/57‑1 (MQ=255) cACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCt < 1:646278/51‑1 (MQ=255) cACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCt < 1:1071203/51‑1 (MQ=255) cACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAgg > 1:226971/1‑68 (MQ=255) cACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATg > 1:1266235/1‑71 (MQ=255) ttAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTa > 1:3430590/1‑51 (MQ=255) ttAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTa > 1:2447825/1‑51 (MQ=255) ttAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTa > 1:2303955/1‑51 (MQ=255) ttAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTa > 1:1026100/1‑51 (MQ=255) ttAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGatat < 1:2098967/56‑1 (MQ=255) ttAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGatat < 1:3544294/56‑1 (MQ=255) ttAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGt > 1:2664132/1‑71 (MQ=255) ttAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGt > 1:3501993/1‑71 (MQ=255) tAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGt > 1:4002580/1‑70 (MQ=255) tAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGTTGGTAGATATCACTGAGGATGTCGTTATCAGaa < 1:3105924/71‑1 (MQ=255) tAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGTTGGTAGATATCACTGAGGATGTCGTTATCAGaa < 1:267903/71‑1 (MQ=255) gggTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGc > 1:3222930/1‑71 (MQ=255) gTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGa < 1:350139/41‑1 (MQ=255) gTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCtt > 1:270915/1‑71 (MQ=255) gTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCt > 1:3694793/1‑70 (MQ=255) gCCACTGACCACATAATTGATCGTTTGTTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAAcc > 1:3093575/1‑70 (MQ=255) gCCACTGACCACATAATTGATCGTTTGTTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAAcc > 1:1651626/1‑70 (MQ=255) cACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGtt < 1:153019/52‑1 (MQ=255) cACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGtt < 1:1885152/52‑1 (MQ=255) cTGAGCACATAATTGAACGTTTGCTGTTAGATATCACTg < 1:1410884/39‑1 (MQ=37) cTGACCACATAATTGATCGTTTGCTGGTAGATATCACTg < 1:3892334/39‑1 (MQ=255) cTGACCACATAATTGATCGTTTGCTGGTAGATATCACTg < 1:2870684/39‑1 (MQ=255) cTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCAttt > 1:4073094/1‑71 (MQ=255) cTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCAttt > 1:1377729/1‑71 (MQ=255) ttaCCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGt < 1:1280003/46‑1 (MQ=255) tGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGt < 1:2285068/48‑1 (MQ=255) gACCACATAATTGATCGTTTGTTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTgg > 1:2601714/1‑71 (MQ=255) gACCACATAATTGATCGTTTGTTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTgg > 1:2028667/1‑71 (MQ=255) gACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTg > 1:1751540/1‑70 (MQ=255) aCCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTg < 1:3674153/69‑1 (MQ=255) aCCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTg < 1:347270/69‑1 (MQ=255) aCCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTg < 1:2915985/69‑1 (MQ=255) aCCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTg < 1:1662326/69‑1 (MQ=255) aCCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTg < 1:2327487/69‑1 (MQ=255) caTAATTGATCGTTTGTTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAAc > 1:1015834/1‑58 (MQ=255) caTAATTGATCGTTTGTTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTc < 1:2572135/68‑1 (MQ=255) caTAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTAt > 1:1382532/1‑45 (MQ=255) caTAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTAt > 1:1103569/1‑45 (MQ=255) aTAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTc < 1:1823325/39‑1 (MQ=255) aTAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTc < 1:2733948/39‑1 (MQ=255) aTAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTc < 1:3370905/39‑1 (MQ=255) aTAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGt > 1:3996295/1‑41 (MQ=255) aTAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGt > 1:736539/1‑66 (MQ=255) aaTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTc > 1:1980015/1‑70 (MQ=255) aaTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTc > 1:475100/1‑70 (MQ=255) aTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAg > 1:2298625/1‑44 (MQ=255) aTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAg > 1:2428456/1‑44 (MQ=255) aTTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAg > 1:3417894/1‑44 (MQ=255) gATCGTTTGTTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCAt > 1:2166541/1‑69 (MQ=255) gATCGTTTGTTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTACAt > 1:1605527/1‑69 (MQ=255) gATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCAt > 1:641883/1‑69 (MQ=255) aTCGTTTGTTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:3863198/71‑1 (MQ=255) aTCGTTTGTTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:1150873/71‑1 (MQ=255) aTCGTTTGTTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:2825237/71‑1 (MQ=255) aTCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATgag < 1:1452767/71‑1 (MQ=255) tGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGt > 1:225526/1‑70 (MQ=255) | AGGTTGCCGATCAAGCATAGCACCTTAAAGCGTAGGGTGCTGGCCACTGACCACATAATTGATCGTTTGCTGGTAGATATCACTGAGGATGTCGTTATCAGAAGCTTCAACCCATTTGGTCAGCTCCATGAGAATGT > W3110S.gb/1557755‑1557891 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |