| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,594,973 | A→G | 10.2% | pseudogene (807/1401 nt) | ydeU ← | ECK1502:JW1502:b1509; conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,594,973 | 0 | A | G | 10.2% | 150.6 / 9.5 | 59 | pseudogene (807/1401 nt) | ydeU | ECK1502:JW1502:b1509; conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (36/17); new base G (4/2); total (40/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
TATCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTTATAACCCACACGAT > W3110S.gb/1594909‑1595040 | tATCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGGTACCAg > 1:714935/1‑71 (MQ=255)tATCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGGTACCAg > 1:694386/1‑71 (MQ=255) aaaCCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATa > 1:2289851/1‑63 (MQ=255) aaaCCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATAc > 1:2979198/1‑64 (MQ=255) aaaCCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATAc > 1:2983935/1‑64 (MQ=255) aaCCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTcgc < 1:1905400/71‑1 (MQ=255) aCCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTcgcg < 1:894632/71‑1 (MQ=255) cAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTa < 1:239841/71‑1 (MQ=255) cAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTa < 1:3553586/71‑1 (MQ=255) ttATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTa < 1:1919783/68‑1 (MQ=255) ataCAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGGTACCAGGTCGCGTACAGcc > 1:1626679/1‑71 (MQ=255) ataCAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGcc > 1:1957080/1‑71 (MQ=255) ataCAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGcc > 1:2341776/1‑71 (MQ=255) ataCAGAGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGca > 1:9095/1‑70 (MQ=255) taCAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGcc > 1:2014111/1‑70 (MQ=255) aCAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGcc > 1:1742898/1‑69 (MQ=255) cgcCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATa > 1:715466/1‑48 (MQ=255) gCTGTCAACATAAGCGCC‑GTCTTATTCGCATCGTTCTGa < 1:371447/39‑1 (MQ=38) tGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATa > 1:772609/1‑40 (MQ=255) tGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATa > 1:1115320/1‑40 (MQ=255) tGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATa > 1:2888725/1‑40 (MQ=255) tCAACGTAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt > 1:4029839/1‑71 (MQ=255) tCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGGTACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt < 1:1183239/71‑1 (MQ=255) tCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGGTACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt < 1:1417692/71‑1 (MQ=255) tCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGc > 1:2388932/1‑70 (MQ=255) tCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt < 1:2653178/71‑1 (MQ=255) tCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt < 1:819788/71‑1 (MQ=255) tCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt < 1:2877547/71‑1 (MQ=255) tCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt > 1:2340690/1‑71 (MQ=255) tCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt < 1:773579/71‑1 (MQ=255) cAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt < 1:1410047/70‑1 (MQ=255) aaCATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCa > 1:950701/1‑39 (MQ=255) aaCATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCa > 1:3120767/1‑39 (MQ=255) aaCATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCa > 1:2681959/1‑39 (MQ=255) aaCATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGt > 1:2261868/1‑47 (MQ=255) aaCATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCt > 1:1638405/1‑60 (MQ=255) aaCATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCt > 1:1637782/1‑69 (MQ=255) aaGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATgcgc > 1:2539925/1‑71 (MQ=255) aGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTg > 1:2815375/1‑55 (MQ=255) aGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGcc > 1:234052/1‑71 (MQ=255) aGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGcc > 1:54839/1‑71 (MQ=255) aGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGcc > 1:2394252/1‑71 (MQ=255) cgcCGGTCTTATTCGCATCTTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCa < 1:3332752/71‑1 (MQ=255) gcCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCa < 1:1577995/70‑1 (MQ=255) gcCGGTCTTATTCGAATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCa < 1:538898/70‑1 (MQ=255) cGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGc < 1:3254642/57‑1 (MQ=255) ggTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAAc > 1:331544/1‑53 (MQ=255) aTTGGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTta > 1:3698374/1‑70 (MQ=255) aTTCGCATCGTTCTGGTACCAGGTCGCGTACAGCCCAGCGCTGTAAcc > 1:1552408/1‑48 (MQ=255) aTTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGAtt > 1:2114354/1‑68 (MQ=255) aTTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTta > 1:4052495/1‑70 (MQ=255) cGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCt > 1:309453/1‑39 (MQ=255) cGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCt > 1:1697324/1‑39 (MQ=255) cGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCt > 1:3557511/1‑39 (MQ=255) gTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTTATAACCCacac > 1:522123/1‑71 (MQ=255) gTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTTATAACCCacac < 1:727833/71‑1 (MQ=255) gTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTTATAACCCacac < 1:3737855/71‑1 (MQ=255) gTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTTATAACCCacac < 1:3699518/71‑1 (MQ=255) cTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGcc > 1:2276251/1‑47 (MQ=255) cTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCAATCCGATTTATAACCCACACGAt > 1:3565085/1‑71 (MQ=255) | TATCAAACCAGTTATACAGCGCCCAGCTGTCAACATAAGCGCCGGTCTTATTCGCATCGTTCTGATACCAGGTCGCGTACAGCCCAGCGCTGTAACCGCTGATGCGCCCATCCGATTTATAACCCACACGAT > W3110S.gb/1594909‑1595040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |