| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,600,711 | A→G | 20.5% | S405P (TCA→CCA) | ydeV ← | predicted sugar kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,600,711 | 0 | A | G | 20.5% | 78.0 / 17.3 | 39 | S405P (TCA→CCA) | ydeV | predicted sugar kinase |
| Reads supporting (aligned to +/- strand): ref base A (14/17); new base G (4/4); total (18/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
CCGCCTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGTCAATGGA > W3110S.gb/1600641‑1600780 | ccGCCTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGa > 1:1075266/1‑71 (MQ=255)ccGCCTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGa > 1:964143/1‑71 (MQ=255)ccGCCTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGa > 1:3001596/1‑71 (MQ=255) cTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACa < 1:1218422/71‑1 (MQ=255) aaaGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATcg < 1:3284446/71‑1 (MQ=255) aCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCAttt > 1:1169776/1‑71 (MQ=255) aCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCAttt > 1:3952452/1‑71 (MQ=255) aCGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATA‑AATCGCCGCAtttt > 1:265528/1‑71 (MQ=255) aTGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTttct < 1:2437056/71‑1 (MQ=255) tGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGGTACa < 1:2301241/55‑1 (MQ=255) gAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTttcttc > 1:3147052/1‑71 (MQ=255) tGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCAttt < 1:4084180/60‑1 (MQ=255) tatTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACg > 1:190292/1‑70 (MQ=255) ttCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATcgccgc < 1:1399200/50‑1 (MQ=255) ttCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATcgccgc < 1:2722049/50‑1 (MQ=255) ttCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATcgccgc < 1:4026531/50‑1 (MQ=255) cGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGGTACAATCGCCGCATTTTCTTCCAGCGCACGGAAc < 1:3081216/70‑1 (MQ=255) aGCAATTTGCTGCAAGTTACACGCTGGTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCtt > 1:199982/1‑71 (MQ=255) aGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACaa > 1:3237690/1‑63 (MQ=255) aGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCCTCCAGCGCACGGAACaa > 1:1470589/1‑63 (MQ=255) aaTTTGCTGCAAGTTACACGCTGGTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGt < 1:2432964/71‑1 (MQ=255) tttGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGt > 1:1657332/1‑69 (MQ=255) tttGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTa < 1:3952494/71‑1 (MQ=255) tttGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTa < 1:1637608/71‑1 (MQ=255) tttGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTa < 1:2513975/71‑1 (MQ=255) tttGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTa < 1:2717988/71‑1 (MQ=255) tgctgcAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACa > 1:3084192/1‑71 (MQ=255) tgctgcAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACa > 1:1637417/1‑71 (MQ=255) tgctgcAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACa > 1:1522866/1‑71 (MQ=255) ctgcAAGTTACACGCTTGTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACa < 1:1925082/69‑1 (MQ=255) cAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACAttt < 1:1410306/69‑1 (MQ=255) cAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTa < 1:3298074/70‑1 (MQ=255) aGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATcc < 1:356018/71‑1 (MQ=255) gTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAAc < 1:626026/46‑1 (MQ=255) tACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCggg > 1:50604/1‑71 (MQ=255) acacGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGt > 1:3113335/1‑71 (MQ=255) cacGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGTc < 1:2554408/71‑1 (MQ=255) cacGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGTc < 1:500572/71‑1 (MQ=255) gCTGGTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCg > 1:2208368/1‑46 (MQ=255) gCTGGTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCg > 1:419731/1‑46 (MQ=255) gCTGGTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCg > 1:658108/1‑46 (MQ=255) gATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGTCAATGGa > 1:2226293/1‑71 (MQ=255) aTACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGTCAATgg < 1:4045216/69‑1 (MQ=255) | CCGCCTGCAAAGACTAACGATGAAGGATGAATATTCGAGAAATCAGCAATTTGCTGCAAGTTACACGCTGATACAATCGCCGCATTTTCTTCCAGCGCACGGAACAATGTCGCTTTGTTACATTTATCCGGGTCAATGGA > W3110S.gb/1600641‑1600780 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |