| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,608,013 | G→A | 25.0% | G67E (GGA→GAA) | lsrF → | predicted aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,608,013 | 0 | G | A | 25.0% | 57.3 / 15.4 | 32 | G67E (GGA→GAA) | lsrF | predicted aldolase |
| Reads supporting (aligned to +/- strand): ref base G (15/9); new base A (5/3); total (20/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGC > W3110S.gb/1607943‑1608078 | tttAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCgg < 1:1954599/71‑1 (MQ=255) cGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGAc > 1:197347/1‑66 (MQ=255) cGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGaa > 1:2640511/1‑71 (MQ=255) cGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGaa > 1:1917399/1‑71 (MQ=255) gTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACg < 1:2256466/64‑1 (MQ=255) gTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGAACTTGAACGCATTGa > 1:1058645/1‑70 (MQ=255) gTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGAACTTGAACGCATTGa > 1:3869582/1‑70 (MQ=255) gTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGAACTTGAACGCATTGa > 1:2150126/1‑70 (MQ=255) tAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGata > 1:2125234/1‑71 (MQ=255) aaaaCCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGata > 1:2142860/1‑70 (MQ=255) aaaaCCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGata > 1:3848850/1‑70 (MQ=255) aaaCCTTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGAACTTGAACGCATTGAtata < 1:1464079/71‑1 (MQ=255) aTGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGaa > 1:3113936/1‑51 (MQ=255) aTGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGc < 1:601695/71‑1 (MQ=255) gCTGGCTTTTGACCATGGTTATTTTCAGGGACCGCCTACCGAACTTGAACGCATTGATATAAATATCGccc > 1:682205/1‑71 (MQ=255) cTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGcccc > 1:687462/1‑71 (MQ=255) cTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGcccc > 1:2297350/1‑71 (MQ=255) cTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGcccc > 1:2386238/1‑71 (MQ=255) cTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTg > 1:3699934/1‑71 (MQ=255) gACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTg < 1:762681/70‑1 (MQ=255) aCCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGaa < 1:3170769/71‑1 (MQ=255) aCCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGaa < 1:1596726/71‑1 (MQ=255) ccATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGaa < 1:3091174/70‑1 (MQ=255) aTGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGAtata < 1:3919406/47‑1 (MQ=255) aTGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGAtata < 1:1679997/47‑1 (MQ=255) gTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGcc > 1:3486876/1‑71 (MQ=255) gTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGcc > 1:4038446/1‑71 (MQ=255) gTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGcc > 1:2078286/1‑71 (MQ=255) gTTATTTTCAGGGACCGACTACCGAACTTGAAGGCATTGATATAAATATCGCCCCGCTg < 1:3943934/59‑1 (MQ=255) aTTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAat < 1:2515195/44‑1 (MQ=255) gACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAAtg > 1:714790/1‑71 (MQ=255) aCTACCGAACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACa < 1:793564/50‑1 (MQ=255) tACCGAACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTAcgc > 1:1283769/1‑71 (MQ=255) | TTTAATCCGAAAACGGGTAAAACCGTGATGCTGGCTTTTGACCATGGTTATTTTCAGGGACCGACTACCGGACTTGAACGCATTGATATAAATATCGCCCCGCTGTTTGAACATGCCGATGTATTAATGTGTACGC > W3110S.gb/1607943‑1608078 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |