| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,621,163 | A→G | 22.9% | Q110Q (CAA→CAG) | marR → | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,621,163 | 0 | A | G | 22.9% | 62.6 / 17.6 | 35 | Q110Q (CAA→CAG) | marR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (15/12); new base G (3/5); total (18/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
CCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTG > W3110S.gb/1621095‑1621229 | ccGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAAt < 1:3720261/70‑1 (MQ=255) gAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGc > 1:2399779/1‑70 (MQ=255) ccGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAGTGCCATCa < 1:3302962/71‑1 (MQ=255) aTGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAAt > 1:3385683/1‑69 (MQ=255) gcgcGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGcc < 1:3829417/71‑1 (MQ=255) cTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAGTGCCATCAAt < 1:1687423/52‑1 (MQ=255) cTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAGTGCCATCAAt < 1:2583303/52‑1 (MQ=255) cTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCa > 1:3970894/1‑71 (MQ=255) ggTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAg > 1:773399/1‑53 (MQ=255) ggTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCAc > 1:1328126/1‑70 (MQ=255) aaaCTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGACCAGGACCTGCACCAAg > 1:1853340/1‑70 (MQ=255) tACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTaa > 1:774184/1‑71 (MQ=255) tACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTa > 1:102001/1‑70 (MQ=255) cggcggCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaaaaa < 1:1022657/71‑1 (MQ=255) aaTATGTGAACAATGCCATCAATTAGTTGGCCAGGa < 1:84579/36‑1 (MQ=255) aaTATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCt < 1:1136092/63‑1 (MQ=255) tatGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAg < 1:2431969/45‑1 (MQ=255) tatGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaaaa < 1:97486/56‑1 (MQ=255) tatGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaaaa < 1:1421643/56‑1 (MQ=255) atGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGAc > 1:371662/1‑70 (MQ=255) tgtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaaaaaa > 1:2979526/1‑56 (MQ=255) tgtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaaaaaa > 1:2793403/1‑56 (MQ=255) tgtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACaaaaaa > 1:2732815/1‑56 (MQ=255) tgtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACg > 1:1200789/1‑70 (MQ=255) tgtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACg > 1:1261261/1‑70 (MQ=255) tgtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGa < 1:1895657/71‑1 (MQ=255) tgtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGa < 1:1202468/71‑1 (MQ=255) tgtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGa < 1:3798351/71‑1 (MQ=255) tgtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGCCGGACGa < 1:756310/71‑1 (MQ=255) gtgAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACg > 1:310628/1‑69 (MQ=255) tgAACAGTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAg > 1:404271/1‑71 (MQ=255) tgAACAGTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAg > 1:3668750/1‑71 (MQ=255) tgAACAGTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAg > 1:3177659/1‑71 (MQ=255) aaCAGTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTg < 1:1296476/71‑1 (MQ=255) aCAGTGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTg < 1:650595/70‑1 (MQ=255) | CCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAATGCCATCAATTAGTTGGCCAGGACCTGCACCAAGAATTAACAAAAAACCTGACGGCGGACGAAGTG > W3110S.gb/1621095‑1621229 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |