| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,649,687 | T→C | 43.1% | Y14H (TAT→CAT) | dicA → | predicted regulator for DicB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,649,687 | 0 | T | C | 43.1% | 17.0 / 65.1 | 51 | Y14H (TAT→CAT) | dicA | predicted regulator for DicB |
| Reads supporting (aligned to +/- strand): ref base T (24/5); new base C (10/12); total (34/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01 | |||||||||||
AATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGT > W3110S.gb/1649624‑1649757 | aaTACGATTTAGCATTAGCTAACTATTGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCAtcgtcg > 1:33511/1‑71 (MQ=255)aaTACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCAtcgtcg > 1:415883/1‑71 (MQ=255) aCGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGa > 1:175315/1‑70 (MQ=255) cGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaa > 1:1550538/1‑70 (MQ=255) cGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaa > 1:3487099/1‑70 (MQ=255) cGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaa > 1:3314963/1‑70 (MQ=255) cGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaa < 1:3741383/71‑1 (MQ=255) ttAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCAtcgtcg > 1:3121468/1‑63 (MQ=255) ttAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaaaa > 1:792491/1‑69 (MQ=255) ttAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAAcc > 1:3156627/1‑71 (MQ=255) cATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaa < 1:1258946/63‑1 (MQ=255) cATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaa < 1:764237/63‑1 (MQ=255) cATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaa < 1:426841/63‑1 (MQ=255) cATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaa < 1:4044536/63‑1 (MQ=255) cATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaa < 1:3334575/63‑1 (MQ=255) cATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaa < 1:2448773/63‑1 (MQ=255) aTTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaa < 1:134797/62‑1 (MQ=255) aTTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaa < 1:1698843/62‑1 (MQ=255) ttAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGaaaaa > 1:1713885/1‑63 (MQ=255) aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAaca < 1:2466545/70‑1 (MQ=255) aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAaca < 1:130775/70‑1 (MQ=255) aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAaca > 1:3961647/1‑70 (MQ=255) aGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAaca > 1:1927283/1‑70 (MQ=255) cTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaa < 1:2384957/57‑1 (MQ=255) cTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaa < 1:3097648/53‑1 (MQ=255) tATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCa > 1:1977363/1‑69 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaaaa > 1:657704/1‑53 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaaaa > 1:2586262/1‑53 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCaa > 1:1397198/1‑69 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAg > 1:2193851/1‑71 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAg > 1:1071665/1‑71 (MQ=255) aTGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAg > 1:428281/1‑71 (MQ=255) tGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGaaaaa > 1:3848024/1‑52 (MQ=255) tGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAAc > 1:2894645/1‑53 (MQ=255) aaaCAAAAAATTTAACTATCGGCGAACGAATCAGGTAtcg > 1:3918376/1‑40 (MQ=255) aaaaaTTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGc > 1:3437249/1‑71 (MQ=255) aaaaaTTTAACTATCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGc > 1:3441823/1‑71 (MQ=255) tttAACTATCGGCGAACGCATCAGGTATCGTCGGaaaa > 1:4071210/1‑38 (MQ=255) tttAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACCCCCaaa > 1:2132076/1‑56 (MQ=255) tttAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCaaa > 1:1515582/1‑56 (MQ=255) tttAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCaaa > 1:3163289/1‑56 (MQ=255) tttAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAg > 1:2177832/1‑71 (MQ=255) tCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGaa < 1:1284847/70‑1 (MQ=255) tCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGaaa < 1:205671/71‑1 (MQ=255) tCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGaaa < 1:729072/71‑1 (MQ=255) tCGGCGAACGCATCAGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGaaa < 1:752801/71‑1 (MQ=255) ggCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGaaaa > 1:58674/1‑70 (MQ=255) cGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTcc > 1:2962591/1‑69 (MQ=255) aGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTc > 1:1695353/1‑71 (MQ=255) aGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTc > 1:2305254/1‑71 (MQ=255) aGGCATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTc > 1:3825474/1‑71 (MQ=255) tATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGt < 1:2855152/71‑1 (MQ=255) | AATACGATTTAGCATTAGCTAACTATGGAAACAAAAAATTTAACTATCGGCGAACGCATCAGGTATCGTCGGAAAAACCTCAAACACACCCAAAGGTCTCTTGCTAAAGCCCTGAAAATCTCCCATGTGTCTGT > W3110S.gb/1649624‑1649757 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |