Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,656,712	C→A	19.5%	Q48H (CAG→CAT)	rspA ←	predicted dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,656,712	0	C	A	19.5%	126.7 / 12.8	46	Q48H (CAG→CAT)	rspA	predicted dehydratase
Reads supporting (aligned to +/- strand):  ref base C (17/20);  new base A (4/5);  total (21/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.96e-01

ATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGTAATACCG  >  W3110S.gb/1656659‑1656778                                                      |                                                                   atatCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCa                                                                <  1:3876591/58‑1 (MQ=255)  tatCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCATGCAAATAAGAGGCCACg                                                  >  1:3759709/1‑71 (MQ=255)  tatCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCATGCAAATAAGAGGCCACg                                                  >  1:1371088/1‑71 (MQ=255)   atCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACg                                                  >  1:570209/1‑70 (MQ=255)   atCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACg                                                  >  1:3261715/1‑70 (MQ=255)   atCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACg                                                  >  1:1980064/1‑70 (MQ=255)   atCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACg                                                  >  1:416682/1‑70 (MQ=255)    tCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACgg                                                 >  1:1768879/1‑70 (MQ=255)     cTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGa                                                >  1:3365171/1‑70 (MQ=255)      ttCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCATGCAAATAAGAGGCCACGGa                                                >  1:300905/1‑69 (MQ=255)        cGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCATGCAAATAAGAGGCCACGGa                                                <  1:1843732/67‑1 (MQ=255)        cGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCATGCAAATAAGAGGCCACGGa                                                <  1:1890004/67‑1 (MQ=255)         gATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGc                                            <  1:2829907/70‑1 (MQ=255)         gATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGc                                            <  1:3246011/70‑1 (MQ=255)                     aTCGCGACCAATAAGCTGCGGACAAAGGTGATCATGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGa                               <  1:3312247/71‑1 (MQ=255)                      tCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAg                              <  1:2082243/71‑1 (MQ=255)                      tCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAg                              <  1:1650866/71‑1 (MQ=255)                      tCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAg                              <  1:2668124/71‑1 (MQ=255)                      tCGCGACCAATAAGCTGCGGACAAAGGTGATCATGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTg                                <  1:3829859/69‑1 (MQ=255)                      tCGCGACCAATAAGCTGCGGACAAAGGTGATCATGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAg                              <  1:2394728/71‑1 (MQ=255)                        gcgACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAggg                            >  1:2095966/1‑71 (MQ=255)                        gcgACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAggg                            >  1:1783874/1‑71 (MQ=255)                            ccAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTc                                     <  1:1780322/58‑1 (MQ=255)                            ccAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTc                                     <  1:474942/58‑1 (MQ=255)                             cAATAAGCCGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCa                       <  1:2173036/71‑1 (MQ=255)                              aaTAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCAt                      <  1:2807741/71‑1 (MQ=255)                              aaTAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCAt                      <  1:1943610/71‑1 (MQ=255)                               aTAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAg                              <  1:2097863/62‑1 (MQ=255)                               aTAAGCTGCGGACAAAGGTGATCATGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAggg                            >  1:2129816/1‑64 (MQ=255)                                tAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCt                                           <  1:1404819/48‑1 (MQ=255)                                 aaGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAg                              <  1:493891/60‑1 (MQ=255)                                     tGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAg                              <  1:3452921/56‑1 (MQ=255)                                     tGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAg                              <  1:3759704/56‑1 (MQ=255)                                       cGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGc             <  1:1799183/71‑1 (MQ=255)                                           cAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGt         <  1:4038119/71‑1 (MQ=255)                                            aaaGTTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCAt                                  >  1:2443491/1‑45 (MQ=255)                                            aaaGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTcc                                    >  1:3083395/1‑43 (MQ=255)                                            aaaGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCAt                                  >  1:615574/1‑45 (MQ=255)                                            aaaGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCAt                                  >  1:2908507/1‑45 (MQ=255)                                            aaaGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCAt                                  >  1:3534276/1‑45 (MQ=255)                                            aaaGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCAt                                  >  1:1829936/1‑45 (MQ=255)                                              aGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGt         <  1:3649234/68‑1 (MQ=255)                                              aGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGt         <  1:826715/68‑1 (MQ=255)                                                  gATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTg                          >  1:3977935/1‑47 (MQ=255)                                                  gATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTg                          >  1:2197666/1‑47 (MQ=255)                                                  gATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGTAATACCg  >  1:659895/1‑71 (MQ=255)                                                      |                                                                   ATATCTTCGATACGGTGCGCATCGCGACCAATAAGCTGCGGACAAAGGTGATCCTGCAAATAAGAGGCCACGGAAAGCTCACGTCCATTGAGGGTGGCATCCCCAAGGCCCGTAATACCG  >  W3110S.gb/1656659‑1656778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑