| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,662,320 | A→G | 13.9% | R17R (AGA→AGG) | ynfF → | oxidoreductase subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,662,320 | 0 | A | G | 13.9% | 89.2 / 7.8 | 36 | R17R (AGA→AGG) | ynfF | oxidoreductase subunit |
| Reads supporting (aligned to +/- strand): ref base A (8/23); new base G (2/3); total (10/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.65e-01 | |||||||||||
AGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGCAGTGCTTTCACTCTGCCATTTTCC > W3110S.gb/1662252‑1662389 | aGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGGAg < 1:1260813/71‑1 (MQ=255) aaTAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCt < 1:2773538/71‑1 (MQ=255) aTAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCtg < 1:3607754/71‑1 (MQ=255) aaGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCtgat > 1:4001649/1‑71 (MQ=255) aGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGGAGTCtgat > 1:3739524/1‑70 (MQ=255) tGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGaa < 1:1154625/71‑1 (MQ=255) tGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGaa < 1:1968883/71‑1 (MQ=255) aGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGGAGTCTGATGaaa < 1:3985638/70‑1 (MQ=255) aGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGaaa < 1:865983/70‑1 (MQ=255) aaTCCATACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGaaa < 1:1473189/58‑1 (MQ=255) cATACAACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAg < 1:2406729/71‑1 (MQ=255) aTACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGt < 1:3156078/71‑1 (MQ=255) aTACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGt < 1:3160674/71‑1 (MQ=255) ccACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCAc > 1:3617214/1‑60 (MQ=255) ccACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCAc > 1:2721990/1‑60 (MQ=255) acaGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCTGCACTTGGCAGTCTTgc < 1:2666972/71‑1 (MQ=255) acaGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTgc < 1:2757727/71‑1 (MQ=255) caGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTgcg < 1:2449715/71‑1 (MQ=255) agagGCGCTCATGAAGGCTGAAATCAGCCGTAGGAGTCTGATGAAAACCTCCGCACTTGGCAGTCt < 1:3413283/66‑1 (MQ=255) tCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGt < 1:584266/56‑1 (MQ=255) tCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGt < 1:3781064/56‑1 (MQ=255) tCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTACGCACTTGGCAGt < 1:1194777/56‑1 (MQ=255) aTGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGc < 1:1525590/69‑1 (MQ=255) aTGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGc < 1:2064658/69‑1 (MQ=255) aTGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGCAg > 1:1460358/1‑71 (MQ=255) tGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCa < 1:3962817/44‑1 (MQ=255) tGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCa < 1:1307521/44‑1 (MQ=255) gAAGGCTGAAATCAGCCGTAGGAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGCAGTg > 1:2471061/1‑71 (MQ=255) gAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGCAg > 1:332020/1‑69 (MQ=255) gAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGCAg > 1:407411/1‑69 (MQ=255) gCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGCAGTGCttt < 1:3661330/71‑1 (MQ=255) aaTCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGCAGTGCttt < 1:69134/66‑1 (MQ=255) aGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGCAGTGCTTTCACTCTGc > 1:2313090/1‑70 (MQ=255) tAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGCAGt < 1:1354951/52‑1 (MQ=255) tAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGCAGt < 1:52114/52‑1 (MQ=255) gAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGCAGTGCTTTCACTCTGCCATTTTcc > 1:1530721/1‑71 (MQ=255) | AGCAATAAGTGAGTAATGATGAAAATCCATACCACAGAGGCGCTCATGAAGGCTGAAATCAGCCGTAGAAGTCTGATGAAAACCTCCGCACTTGGCAGTCTTGCGCTGGCAAGCAGTGCTTTCACTCTGCCATTTTCC > W3110S.gb/1662252‑1662389 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |