| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,672,743 | A→G | 36.0% | intergenic (+77/‑347) | ynfM → / → asr | predicted transporter/acid shock‑inducible periplasmic protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,672,743 | 0 | A | G | 36.0% | 20.6 / 20.7 | 25 | intergenic (+77/‑347) | ynfM/asr | predicted transporter/acid shock‑inducible periplasmic protein |
| Reads supporting (aligned to +/- strand): ref base A (11/5); new base G (5/4); total (16/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGTCCGGACTGCGGTAAATACCCGTCCGGACTTATTGCCAGCTCAAACCAACGTTAATAGCCATCCTAAAATAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGATTATAAGTACCCAAATAAACG > W3110S.gb/1672673‑1672812 | aGTCCGGACTGCGGTAAATACCCGTCCGGACTTATTGCCAGCTCAAACCAACGTTAATAGCCATCCTaaaa > 1:3754044/1‑71 (MQ=255) cGGACTGCGGTAAATACCCGTCCGGACTTATTGCCAGCTCAAACCAACGTTAATAGCCATCCTAAAATAg < 1:3720802/70‑1 (MQ=255) cGGACTGCGGTAAATACCCGTCCGGACTTATTGCCAGCTCAAACCAACGTTAATAGCCATCCTAAAATAg < 1:681767/70‑1 (MQ=255) aaaTACACGTCCGGACTTATTGCCAGCTCAAACCAACGTTAATAGCCATCCTAAAGTAGACGAAGCgccag > 1:3969605/1‑71 (MQ=255) aCCCGTCCGGACTTATTGCCAGCTCAAACCAACGTTAATAGCCATCCTaaaa > 1:420696/1‑52 (MQ=255) aCCCGTCCGGACTTATTGCCAGCTCAAACCAACGTTAATAGCCATCCTAAAATAGACGAAGCGCCAGCCaa > 1:483047/1‑71 (MQ=255) aCCCGTCCGGACTTATTGCCAGCTCAAACCAACGTTAATAGCCATCCTAAAATAGACGAAGCGCCAGCCaa > 1:719635/1‑71 (MQ=255) cGGACTTATTGCCAGCTCAAACCAACGTTAATAGCCATCCTAAAGTAGACGAAGCGCCAGCCAATTCCCg > 1:3292841/1‑70 (MQ=255) ttATTGCCAGCTCAAACCAACGTTAATAGCCATCCTAAAATAGACGAAGCGCCAGCCaa < 1:1770013/59‑1 (MQ=255) aTTGCCAGCTCAAACCAACGTTAATAGACATCCTAAAGTAGACGAAGCGCCAGCCAATTCCCGCAGCGCGt > 1:2151800/1‑71 (MQ=255) ccAGCTCAAACCAACGTTAATAGCCATCCTAAAGTAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTa < 1:2849920/70‑1 (MQ=255) gCTCAAACCAACGTTAATAGCCATCCTAAAATAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGt > 1:3747001/1‑71 (MQ=255) cTCAAACCAACGTTAATAGCCATCCTAAAATAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGt > 1:3179892/1‑70 (MQ=255) cAAACCAACGTTAATAGCCATCCTAAAGTAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGtcat > 1:1561420/1‑71 (MQ=255) aCCAACGTTAATAGCCATCCTAAAGTAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTc < 1:3350149/59‑1 (MQ=255) ccAACGTTAATAGCCATCCTAAAATAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAg > 1:296495/1‑70 (MQ=255) ccAACGTTAATAGCCATCCTAAAATAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAg > 1:1042666/1‑70 (MQ=255) aCGTTAATAGCCATCCTAAAATAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGAt > 1:2027140/1‑70 (MQ=255) aCGTTAATAGCCATCCTAAAATAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGAt > 1:2630717/1‑70 (MQ=255) aCGTTAATAGCCATCCTAAAATAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGAt > 1:2535613/1‑70 (MQ=255) gTTAATAGCCATCCTAAAGTAGACGAAGCGCCAGCCAATTcc < 1:3725463/42‑1 (MQ=255) gTTAATAGCCATCCTAAAATAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGATtat < 1:445558/71‑1 (MQ=255) ttAATAGCCATCCTAAAATAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGATtata < 1:758241/71‑1 (MQ=255) aaTAGCCATCCTAAAATAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGATTATAAg > 1:3245027/1‑71 (MQ=255) aaTAGCCATCCTAAAATAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGATTATAAg > 1:1669019/1‑71 (MQ=255) tCCTAAAGTAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGATta < 1:2079180/59‑1 (MQ=255) cTAAAGTAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGATTATAAGTACCCAAAt > 1:1992222/1‑70 (MQ=255) aaaaTAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGATTATAAGTACCCAAATaaa > 1:4013909/1‑71 (MQ=255) aaaaTAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGATTATAAGTACCCAAATaaa > 1:2885984/1‑71 (MQ=255) aaaTAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGATTATAAGTACCCaa > 1:579229/1‑65 (MQ=255) aTAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGATTATAAGTACCCAAATAAACg > 1:2974587/1‑70 (MQ=255) | AGTCCGGACTGCGGTAAATACCCGTCCGGACTTATTGCCAGCTCAAACCAACGTTAATAGCCATCCTAAAATAGACGAAGCGCCAGCCAATTCCCGCAGCGCGTCTAGCGTCATCAGGATTATAAGTACCCAAATAAACG > W3110S.gb/1672673‑1672812 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |