| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,696,825 | T→C | 19.6% | I321V (ATT→GTT) | uidA ← | beta‑D‑glucuronidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,696,825 | 0 | T | C | 19.6% | 97.1 / 20.0 | 46 | I321V (ATT→GTT) | uidA | beta‑D‑glucuronidase |
| Reads supporting (aligned to +/- strand): ref base T (19/18); new base C (7/2); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01 | |||||||||||
TGTTCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGCAAGTCCGCAT > W3110S.gb/1696755‑1696893 | tGTTCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAAt > 1:3885078/1‑71 (MQ=255) ttCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATc > 1:3053191/1‑70 (MQ=255) ttCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATc > 1:145213/1‑70 (MQ=255) ttCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATc > 1:1628705/1‑70 (MQ=255) cATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAg > 1:3638629/1‑71 (MQ=255) cATCTGCCCAGTCGAGCATCTCTTCACCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCa < 1:3238624/70‑1 (MQ=255) cccAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCAtt < 1:3362637/71‑1 (MQ=255) cccAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCAtt < 1:416967/71‑1 (MQ=255) cccAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAACCCAGTc < 1:2517469/67‑1 (MQ=255) cccAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAACCCAGTCCAtt < 1:3276895/71‑1 (MQ=255) gTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCAtt < 1:1561536/67‑1 (MQ=255) tCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTc < 1:3319502/62‑1 (MQ=255) gAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCg < 1:1113700/70‑1 (MQ=255) gAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCg < 1:160000/70‑1 (MQ=255) cATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTgg > 1:794387/1‑70 (MQ=255) cATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGt > 1:3358735/1‑71 (MQ=255) cATCTCTTCAGCGTAAGGG‑AATGCGAGGTACGGTAGGAGGTGGCCCCAATcc < 1:19386/52‑1 (MQ=255) tctcTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTc > 1:581348/1‑55 (MQ=255) tctcTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTc < 1:2535130/55‑1 (MQ=255) tctTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGt > 1:2851240/1‑70 (MQ=255) tctTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAACCCAGTCCATTAATGCGTGGTCGTg > 1:442574/1‑71 (MQ=255) tctTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAACCCAGTCCATTAATGCGTGGTCGTg > 1:1329599/1‑71 (MQ=255) ctTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAACCCa > 1:114081/1‑49 (MQ=255) ctTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAACCCa > 1:1232477/1‑49 (MQ=255) cAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGt > 1:421353/1‑63 (MQ=255) cAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCAc < 1:1864853/70‑1 (MQ=255) cAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCAc > 1:3363430/1‑70 (MQ=255) cAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCAc > 1:1239597/1‑70 (MQ=255) cAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCAc > 1:1359577/1‑70 (MQ=255) cAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCAc < 1:2198081/70‑1 (MQ=255) aGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCa > 1:97360/1‑71 (MQ=255) gTAATGCGAGGTACGGTAGGAGTTGGCCCCAACCCAGTCCATTaa > 1:1003259/1‑45 (MQ=255) gTAATGCGAGGTACGGTAGGAGTTGGCCCCAACCCAGTCCATTaa > 1:2042690/1‑45 (MQ=255) gCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTc < 1:989113/49‑1 (MQ=255) cGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATc > 1:941122/1‑69 (MQ=255) cGAGGTACGGTAGGAGTTGGCCACAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCg > 1:612725/1‑70 (MQ=255) gAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGa < 1:1326554/70‑1 (MQ=255) gTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGc < 1:22824/38‑1 (MQ=255) gTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATc > 1:315103/1‑70 (MQ=255) cGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCttt > 1:3331752/1‑71 (MQ=255) gTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGc < 1:1654447/71‑1 (MQ=255) tAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGa < 1:2803205/61‑1 (MQ=255) aGGAGTTGGCCCCAACCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCa > 1:362813/1‑71 (MQ=255) gTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGc > 1:1636703/1‑37 (MQ=255) ggCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGCAAg > 1:3092621/1‑70 (MQ=255) gCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGCAAGt < 1:1934973/70‑1 (MQ=255) aaTCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGCAAGTCCGCAt < 1:2382623/71‑1 (MQ=255) | TGTTCATCTGCCCAGTCGAGCATCTCTTCAGCGTAAGGGTAATGCGAGGTACGGTAGGAGTTGGCCCCAATCCAGTCCATTAATGCGTGGTCGTGCACCATCAGCACGTTATCGAATCCTTTGCCACGCAAGTCCGCAT > W3110S.gb/1696755‑1696893 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |