| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,698,594 | A→G | 26.0% | L58S (TTG→TCG) | uidR ← | DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,698,594 | 0 | A | G | 26.0% | 81.0 / 32.9 | 50 | L58S (TTG→TCG) | uidR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (18/19); new base G (5/8); total (23/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01 | |||||||||||
ATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTT > W3110S.gb/1698534‑1698664 | aTCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCGAGGCTTCttt > 1:2995329/1‑71 (MQ=255) ccGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCGAGGCTTCTTTGGAGATGa < 1:2195007/71‑1 (MQ=255) cGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCGAGGCTTCTTTGGAGATGaa < 1:3382209/71‑1 (MQ=255) cGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCGAGGCTTCTTTGGAGATGaa < 1:190840/71‑1 (MQ=255) gAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCGAGGCTTCTTTGgaga > 1:2227698/1‑65 (MQ=255) aCGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGt < 1:3212379/71‑1 (MQ=255) gggCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCGAGGCTTCTTTGGAGATGAAATGGTGa < 1:3166464/71‑1 (MQ=255) gggCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGa < 1:3457100/71‑1 (MQ=255) cgcCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCGAGGCTTCTTTGGAGATGa < 1:2954316/56‑1 (MQ=255) cgcCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCGAGGCTTCTTTGGAGATGa < 1:708921/56‑1 (MQ=255) ctctCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGaaa > 1:3742840/1‑54 (MQ=255) tctcCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATg < 1:805290/55‑1 (MQ=255) tctcCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATg < 1:3627613/55‑1 (MQ=255) ctcCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGa > 1:3263948/1‑58 (MQ=255) ccTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTccc > 1:3030717/1‑67 (MQ=255) cTGGTCCTGTAAGATAATCGCCTGAATCGAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCgg < 1:739813/68‑1 (MQ=255) gTCCTGTAAGATAATCGCCTGAATCGAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTaa > 1:2989381/1‑71 (MQ=255) gTCCTGTAAGATAATCGCCTGAATCGAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTaa > 1:2792140/1‑71 (MQ=255) cTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGaaa > 1:1436583/1‑43 (MQ=255) cTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATaga < 1:1018248/53‑1 (MQ=255) cTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATaga < 1:288349/53‑1 (MQ=255) tGTAAGATAATCGCCTGAATCGAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACt < 1:1372221/65‑1 (MQ=255) tGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCgg < 1:2878076/61‑1 (MQ=255) gTAAGATAATCGCCTGAATCAAGGCTTCTTTGgag > 1:858064/1‑35 (MQ=255) tAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTc > 1:3636519/1‑55 (MQ=255) tAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTc > 1:2480655/1‑55 (MQ=255) tAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACt < 1:2539148/63‑1 (MQ=255) tAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACt < 1:3579611/63‑1 (MQ=255) tAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGgcg > 1:1643623/1‑70 (MQ=255) tAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGgcg > 1:825723/1‑70 (MQ=255) tAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGAt > 1:292761/1‑71 (MQ=255) tAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGAt > 1:1858944/1‑71 (MQ=255) tAATCGCCTGAAT‑AAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGAtt > 1:2152084/1‑71 (MQ=255) aaTCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGAtt > 1:1189608/1‑71 (MQ=255) aaTCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGAtt > 1:3237820/1‑71 (MQ=255) aaTCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGAtt > 1:2365647/1‑71 (MQ=255) aTCGCCTGAATCGAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGAtt > 1:80908/1‑70 (MQ=255) aTCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGa > 1:994014/1‑39 (MQ=255) aTCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGa > 1:2270754/1‑39 (MQ=255) gCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGTCTAATGGCGCAAGATTTACa < 1:3186024/71‑1 (MQ=255) gCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTaa > 1:1460573/1‑55 (MQ=255) gCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACa < 1:2661101/71‑1 (MQ=255) gCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACa < 1:438273/71‑1 (MQ=255) gCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACa < 1:3243588/71‑1 (MQ=255) gCCTGAATCAAGGCTTCTTTAGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACa < 1:985686/71‑1 (MQ=255) ccTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACt < 1:3731892/52‑1 (MQ=255) ccTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACa < 1:550753/70‑1 (MQ=255) tCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACa < 1:2719424/64‑1 (MQ=255) tCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACa < 1:1913409/64‑1 (MQ=255) tCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACa < 1:891026/64‑1 (MQ=255) aaGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCttt > 1:4009139/1‑71 (MQ=255) aaGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCttt > 1:428618/1‑71 (MQ=255) aaGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCttt > 1:1626743/1‑71 (MQ=255) | ATCGGTTCCCGGAAACGGGCCAGCGCCCTCTCCTGGTCCTGTAAGATAATCGCCTGAATCAAGGCTTCTTTGGAGATGAAATGGTGATAGAGCGTCCCGGGACTAATGGCGCAAGATTTACAGATGGCTTT > W3110S.gb/1698534‑1698664 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |