| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,713,297 | A→G | 12.8% | T21A (ACC→GCC) | nth → | DNA glycosylase and apyrimidinic (AP) lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,713,297 | 0 | A | G | 12.8% | 118.0 / 10.0 | 47 | T21A (ACC→GCC) | nth | DNA glycosylase and apyrimidinic (AP) lyase |
| Reads supporting (aligned to +/- strand): ref base A (8/33); new base G (4/2); total (12/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.56e-01 | |||||||||||
TCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGGCGACC > W3110S.gb/1713233‑1713365 | tCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCaccacc > 1:3676162/1‑70 (MQ=255) aaTAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTa < 1:2255979/70‑1 (MQ=255) aaTAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTa < 1:4026602/70‑1 (MQ=255) aTAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTaa < 1:2476468/70‑1 (MQ=255) aTAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTa < 1:3678711/69‑1 (MQ=255) aGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACC‑AGCTTAATTTCAg < 1:1891284/71‑1 (MQ=255) cAAAACGCCTGGAGATCCTCACTCGCCTTCGTGAGAACAATCCTCATCCCACCACCGa < 1:3501180/58‑1 (MQ=255) cAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGa < 1:561929/58‑1 (MQ=255) cTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCtt < 1:3976402/70‑1 (MQ=255) gagaTCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:1751006/70‑1 (MQ=255) aTCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:109830/67‑1 (MQ=255) aTCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:3705180/67‑1 (MQ=255) ccTCACTCGCCTGCGTGAGAACAATCCTCATCCCGCCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGc > 1:419510/1‑71 (MQ=255) ccTCACTCGCCTGCGTGAGAACAATCCTCATCCCGCCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGc > 1:2668684/1‑71 (MQ=255) cTCACTCGCCTGCGTGAGAACAATCCTCATCCcaccac > 1:2476669/1‑38 (MQ=255) cTCACTCGCCTGCGTGAGAACAATCCTCATCCcaccac > 1:2589436/1‑38 (MQ=255) cTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGa < 1:2320926/41‑1 (MQ=255) cTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCt < 1:1780330/71‑1 (MQ=255) cTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCt < 1:2764197/71‑1 (MQ=255) cTCACTCGCCTGAGTGAGAACAATCCTCATCCCACCACCGa < 1:1090553/41‑1 (MQ=255) tCACTCGCCTGCGTGAGAACAATCCTCATCCCGCCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCt > 1:467531/1‑70 (MQ=255) aCTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGAt > 1:3525188/1‑71 (MQ=255) cTCGCCTGCGTGAGAACAATCCTCATCCCGCCACCGAGCTTAAttt < 1:1291921/46‑1 (MQ=255) cTCGCCTGCGTGAGAACAATCCTCATCCCACCACCTAGCtt < 1:1511741/41‑1 (MQ=255) cTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCtt < 1:3469773/41‑1 (MQ=255) cTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCtt < 1:2875122/41‑1 (MQ=255) tCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCtt < 1:3571200/40‑1 (MQ=255) cGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAg > 1:2525327/1‑47 (MQ=255) cGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGaa > 1:272657/1‑60 (MQ=255) cGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGaa > 1:4080017/1‑60 (MQ=255) cTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGa < 1:302632/56‑1 (MQ=255) cTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGa < 1:2115548/56‑1 (MQ=255) cTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCg < 1:3579549/70‑1 (MQ=255) cTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGt < 1:1247185/71‑1 (MQ=255) cTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGt < 1:1723052/71‑1 (MQ=255) tGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCt < 1:498676/61‑1 (MQ=255) tGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCt < 1:720895/61‑1 (MQ=255) tGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCt < 1:1295955/61‑1 (MQ=255) cGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAAt > 1:190576/1‑55 (MQ=255) aaCAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:2594646/46‑1 (MQ=255) aaCAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:1831055/46‑1 (MQ=255) aaCAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:1405532/46‑1 (MQ=255) aaCAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:477791/46‑1 (MQ=255) aaCAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:1250244/46‑1 (MQ=255) aaCAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGAt > 1:2423427/1‑56 (MQ=255) aaCAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGAt > 1:1008241/1‑56 (MQ=255) cTCATCCCGCCACCGAGCTTAATTTCAGTTCGCCTTTTg < 1:150333/39‑1 (MQ=255) cATCCCGCCACCGAGCTTAATTTCAGTTCGCCTTTTGaa > 1:2758271/1‑39 (MQ=255) cATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGaa > 1:3142999/1‑39 (MQ=255) tCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGt < 1:575875/51‑1 (MQ=255) ccaccaCCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGGCGAcc > 1:1012810/1‑71 (MQ=255) ccaccaCCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGGCGAcc < 1:654633/71‑1 (MQ=255) | TCTGATGAATAAAGCAAAACGCCTGGAGATCCTCACTCGCCTGCGTGAGAACAATCCTCATCCCACCACCGAGCTTAATTTCAGTTCGCCTTTTGAATTGCTGATTGCCGTACTGCTTTCCGCTCAGGCGACC > W3110S.gb/1713233‑1713365 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |