| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,769,899 | C→T | 8.3% | S167S (TCG→TCA) | ydiJ ← | predicted FAD‑linked oxidoreductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,769,899 | 0 | C | T | 8.3% | 187.1 / 5.9 | 60 | S167S (TCG→TCA) | ydiJ | predicted FAD‑linked oxidoreductase |
| Reads supporting (aligned to +/- strand): ref base C (32/23); new base T (2/3); total (34/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GAGAATATCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCG > W3110S.gb/1769830‑1769969 | gagaATATCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGc > 1:2673460/1‑70 (MQ=255) gaATATCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGAt < 1:805728/71‑1 (MQ=255) aaTATCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGTGATc > 1:1071559/1‑71 (MQ=255) atatCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATcc < 1:2180251/71‑1 (MQ=255) tCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTg < 1:3324663/71‑1 (MQ=255) tCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTg < 1:449169/71‑1 (MQ=255) cGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTg < 1:3266797/70‑1 (MQ=255) cccccAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCg < 1:1867459/71‑1 (MQ=255) aacaacACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGa > 1:2891455/1‑68 (MQ=255) acaacaCCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCa > 1:1239586/1‑71 (MQ=255) acaacaCCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCa > 1:1597163/1‑71 (MQ=255) aacaCCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATc > 1:692450/1‑71 (MQ=255) aacaCCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATc > 1:1206861/1‑71 (MQ=255) aacaCCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATc > 1:1931190/1‑71 (MQ=255) acacCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCg > 1:340991/1‑71 (MQ=255) acCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGAc > 1:469979/1‑58 (MQ=255) acCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGAc > 1:2782025/1‑58 (MQ=255) acCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGAc > 1:1510415/1‑58 (MQ=255) acCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGAc > 1:1993242/1‑58 (MQ=255) acCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGAc > 1:1086987/1‑58 (MQ=255) ccGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGAt < 1:2757816/50‑1 (MQ=255) ccGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGAt < 1:3023179/50‑1 (MQ=255) gcgcgTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGAt < 1:4016797/48‑1 (MQ=255) cgcgTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTg < 1:3327081/71‑1 (MQ=255) cgcgTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTg < 1:1472656/71‑1 (MQ=255) gCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGTGATCCCTg < 1:1584721/45‑1 (MQ=255) gCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGTGATCCCTg < 1:1149063/45‑1 (MQ=255) gCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATcc < 1:3613971/71‑1 (MQ=255) cAAGTACGTGATCTGACGTTTTGCCATAGACCAGTGATCCCTGAc > 1:1630611/1‑45 (MQ=255) aaGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGa > 1:3121490/1‑48 (MQ=255) aaGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGa > 1:1080479/1‑48 (MQ=255) aaGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGa > 1:2220472/1‑48 (MQ=255) aGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATg > 1:1572399/1‑49 (MQ=255) tACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCgg < 1:545912/53‑1 (MQ=255) cGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCgg > 1:1399215/1‑42 (MQ=255) cGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAg < 1:3465267/70‑1 (MQ=255) cGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAg > 1:815759/1‑70 (MQ=255) cGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGc < 1:3452457/71‑1 (MQ=255) cGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGc > 1:3833355/1‑71 (MQ=255) cGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGc > 1:3986326/1‑71 (MQ=255) aTCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTg < 1:3396608/52‑1 (MQ=255) aTCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTg < 1:669968/52‑1 (MQ=255) tCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTg < 1:3813886/51‑1 (MQ=255) tCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTg < 1:3938935/51‑1 (MQ=255) cTGACGTTTTGCCATAGACCAGCGATCCCTGACCg > 1:50115/1‑35 (MQ=255) cTGACGTTTTGCCATAGACCAGCGATCCCTGACCg > 1:360868/1‑35 (MQ=255) cTGACGTTTTGCCATAGACCAGCGATCCCTGACCg > 1:1996248/1‑35 (MQ=255) cTGACGTTTTGCCATAGACCAGCGATCCCTGACCg > 1:1419103/1‑35 (MQ=255) cTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATcc < 1:2218947/57‑1 (MQ=255) cTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATcc < 1:2034726/57‑1 (MQ=255) cTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCAc > 1:823946/1‑60 (MQ=255) cTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGcc > 1:536498/1‑71 (MQ=255) cTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATAATCCCAc > 1:586756/1‑60 (MQ=255) aCGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGc < 1:3364065/67‑1 (MQ=255) ttttGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGat < 1:3304958/46‑1 (MQ=255) ttGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGat > 1:1229992/1‑44 (MQ=255) ttGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGat > 1:856798/1‑44 (MQ=255) gACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGa > 1:903882/1‑69 (MQ=255) ccAGTGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGt < 1:2044919/71‑1 (MQ=255) aGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCa > 1:4082640/1‑39 (MQ=255) aGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCa > 1:1359582/1‑39 (MQ=255) cGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCg > 1:519026/1‑71 (MQ=255) cGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCg > 1:2161744/1‑71 (MQ=255) | GAGAATATCGCCCCCCAACAACACCGCGCGTACGCCAAGTACGTGATCTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCG > W3110S.gb/1769830‑1769969 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |