| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,796,665 | C→T | 41.2% | A68T (GCG→ACG) | btuC ← | vitamin B12 transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,796,665 | 0 | C | T | 41.2% | 5.3 / 16.1 | 17 | A68T (GCG→ACG) | btuC | vitamin B12 transporter subunit |
| Reads supporting (aligned to +/- strand): ref base C (5/5); new base T (3/4); total (8/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.19e-01 | |||||||||||
GTCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCACGAG > W3110S.gb/1796599‑1796730 | gtCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCacc > 1:348349/1‑70 (MQ=255) gTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGTACCAACCAGc < 1:1809904/71‑1 (MQ=255) tCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCaa > 1:3097120/1‑71 (MQ=255) cTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCaa < 1:287013/70‑1 (MQ=255) ccAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATAc < 1:2978916/70‑1 (MQ=255) ccAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATAc < 1:3083401/70‑1 (MQ=255) aTTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATAcagccagc < 1:374482/70‑1 (MQ=255) ttCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGTACCAACCAGCAATACAGCCAGCGt > 1:1738450/1‑69 (MQ=255) ttCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGTACCAACCAGCAATACAGCCAGCGTAc > 1:48659/1‑71 (MQ=255) ttCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATAca > 1:2253329/1‑61 (MQ=255) aaaCAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGTACCAACCAGCAATACAGCCAGCGTACGTg < 1:1496419/71‑1 (MQ=255) aaaCAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGTACCAACCAGCAATACAGCCAGCGTACGTg > 1:3671327/1‑71 (MQ=255) cTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGaa < 1:1243587/71‑1 (MQ=255) tataGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGtt > 1:791140/1‑71 (MQ=255) tataGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGtt > 1:323240/1‑71 (MQ=255) gCCAGCGCCGTACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGcc < 1:2523577/71‑1 (MQ=255) cAGCGCCGTACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCAc < 1:127564/71‑1 (MQ=255) cgccgcACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCACgag < 1:2441468/71‑1 (MQ=255) cgccgcACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCACgag < 1:1484651/71‑1 (MQ=255) | GTCCAGGTTCTGCCAGAGGATTTTCAAACAACGCCTGCATTACAGCGCCGGATATAGCCAGCGCCGCACCAACCAGCAATACAGCCAGCGTACGTGGCAGGCGAATTTGCCAGACGAACAGTTCGCCACGAG > W3110S.gb/1796599‑1796730 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |