| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,800,301 | A→C | 25.0% | F119C (TTC→TGC) | pheS ← | phenylalanine tRNA synthetase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,800,301 | 0 | A | C | 25.0% | 59.0 / 37.6 | 52 | F119C (TTC→TGC) | pheS | phenylalanine tRNA synthetase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base A (16/23); new base C (5/8); total (21/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCATCGAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGCGACGACCTGGCA > W3110S.gb/1800232‑1800370 | gCATCGAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCgaaga > 1:3638270/1‑70 (MQ=255)gCATCGAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCgaaga > 1:4040290/1‑70 (MQ=255) aTCGAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAAc > 1:3585341/1‑71 (MQ=255) gAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACtt < 1:3310161/70‑1 (MQ=255) gAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACttt > 1:3332966/1‑71 (MQ=255) aaGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTc < 1:3620032/71‑1 (MQ=255) aTGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATAc > 1:2569971/1‑71 (MQ=255) tGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATAc < 1:1056600/70‑1 (MQ=255) gATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACgg > 1:1103935/1‑71 (MQ=255) tAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAAc > 1:1281766/1‑58 (MQ=255) tAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTc < 1:3375176/71‑1 (MQ=255) tAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTc < 1:1635662/71‑1 (MQ=255) aaTCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTc > 1:2142944/1‑70 (MQ=255) aTCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGa < 1:2120692/71‑1 (MQ=255) tCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGAt < 1:921644/71‑1 (MQ=255) gTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATgg > 1:3986706/1‑71 (MQ=255) gTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATgg > 1:2086271/1‑71 (MQ=255) gTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATgg < 1:2962864/71‑1 (MQ=255) gTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATgg < 1:475184/71‑1 (MQ=255) gTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATgg < 1:1349159/71‑1 (MQ=255) tCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGa < 1:1747275/58‑1 (MQ=255) tCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGa < 1:3811799/58‑1 (MQ=255) ttCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTAc > 1:620719/1‑71 (MQ=255) ttCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTAc > 1:3102632/1‑71 (MQ=255) ttCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTAc > 1:3931701/1‑71 (MQ=255) ttCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTAc > 1:2900563/1‑71 (MQ=255) ttCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTAc > 1:2952409/1‑71 (MQ=255) cGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGa > 1:3763693/1‑63 (MQ=255) aTTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGt < 1:1114910/71‑1 (MQ=255) tCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAAc > 1:2124448/1‑71 (MQ=255) cGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCg < 1:399861/71‑1 (MQ=255) cGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCg < 1:700946/71‑1 (MQ=255) cGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCg < 1:31177/71‑1 (MQ=255) cGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCg < 1:2060839/71‑1 (MQ=255) gCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGa > 1:925902/1‑46 (MQ=255) gCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGa > 1:2228157/1‑46 (MQ=255) gTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCg < 1:641870/55‑1 (MQ=255) gTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCg < 1:1629971/55‑1 (MQ=255) gTAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGATGGTACGGGTAACCg < 1:2880966/55‑1 (MQ=255) tAAAGCCAAGCTCACCGAAGCAACTTTCGATACGGTCGAt < 1:3645802/40‑1 (MQ=255) aaaGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGttt > 1:862346/1‑70 (MQ=255) aaaGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGttt > 1:2025473/1‑70 (MQ=255) aaGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTc < 1:1175585/71‑1 (MQ=255) aaGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTc < 1:3882382/71‑1 (MQ=255) aaGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTc < 1:2583908/71‑1 (MQ=255) gCCAAGCTCACCGAAGAAACTTTCGATACGGTCGa > 1:1766837/1‑35 (MQ=255) gCCAAGCTCACCGAAGAAACTTTCGATACGGTCGa > 1:1386877/1‑35 (MQ=255) gCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTc < 1:2961502/69‑1 (MQ=255) cAAGCTCACCGAAGAAACTTTCGATACGGTCTATGGTACGGGTAACCg < 1:1606716/48‑1 (MQ=255) cAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCg < 1:3190011/48‑1 (MQ=255) cAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCg < 1:1370780/48‑1 (MQ=255) cAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCg < 1:3498610/48‑1 (MQ=255) aGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCg < 1:3500097/46‑1 (MQ=255) cGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTc < 1:850972/58‑1 (MQ=255) gaaACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGCGACGACCTGGCa < 1:1627885/71‑1 (MQ=255) aaaCTTTCGATTCGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGCGACGACCTGGCa < 1:3539785/70‑1 (MQ=255) aaaCTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGCGACGACCTGGCa < 1:3049451/70‑1 (MQ=255) | GCATCGAAGTTATGATAATCGTCTTCGATTTCCGGCCCGGTTGCCACGGTAAAGCCAAGCTCACCGAAGAAACTTTCGATACGGTCGATGGTACGGGTAACCGGATGCAGACCGCCGTTTTCAATGCGACGACCTGGCA > W3110S.gb/1800232‑1800370 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |