| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,804,377 | A→G | 21.0% | intergenic (‑93/‑431) | thrS ← / → arpB | threonyl‑tRNA synthetase/ECK1718:JW5278:b1720; hypothetical protein, N‑ter fragment |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,804,377 | 0 | A | G | 21.0% | 167.4 / 42.4 | 81 | intergenic (‑93/‑431) | thrS/arpB | threonyl‑tRNA synthetase/ECK1718:JW5278:b1720; hypothetical protein, N‑ter fragment |
| Reads supporting (aligned to +/- strand): ref base A (34/30); new base G (8/9); total (42/39) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
CCCACACGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTC > W3110S.gb/1804310‑1804438 | cccACACGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCcaaca < 1:2803868/71‑1 (MQ=255)cccACACGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCcaaca < 1:1470092/71‑1 (MQ=255) cacaCGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACaaa > 1:3458070/1‑71 (MQ=255) cacaCGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACaaa > 1:2333900/1‑71 (MQ=255) cacGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATg > 1:1088585/1‑71 (MQ=255) cacGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATg > 1:2632215/1‑71 (MQ=255) cacGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATg > 1:1344903/1‑71 (MQ=255) aaaGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCGACAAATGCTaa < 1:3138435/71‑1 (MQ=255) aaGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCcaaca < 1:1819520/62‑1 (MQ=255) aaGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCcaaca < 1:3710491/62‑1 (MQ=255) aaGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCcaaca < 1:2582096/62‑1 (MQ=255) aaGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCcaaca < 1:2577492/62‑1 (MQ=255) aGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCcaaca < 1:1095777/61‑1 (MQ=255) aGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAAt > 1:3934002/1‑71 (MQ=255) cacaTACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGt > 1:818130/1‑71 (MQ=255) aTACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCcgaca < 1:3871779/54‑1 (MQ=255) tACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCg > 1:1816918/1‑70 (MQ=255) aCAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCg > 1:1627408/1‑69 (MQ=255) aCAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGc > 1:732550/1‑70 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTaaa > 1:1274593/1‑60 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTaaa > 1:1042811/1‑60 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTaaa > 1:1078705/1‑60 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCaa > 1:2302709/1‑71 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCaa > 1:26633/1‑71 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCaa > 1:3835843/1‑71 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCaa > 1:3588677/1‑71 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCaa > 1:1675484/1‑71 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCaa > 1:3846983/1‑71 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCaa > 1:3900671/1‑71 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCaa > 1:3920844/1‑71 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCaa > 1:447532/1‑71 (MQ=255) cAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCaa > 1:980051/1‑71 (MQ=255) gAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAg > 1:2753241/1‑71 (MQ=255) tttGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTacac < 1:3964340/71‑1 (MQ=255) tGTTTATTAACAGTTCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTAcaca < 1:2413985/70‑1 (MQ=255) attaACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGCTACACAAATGa < 1:635363/70‑1 (MQ=255) aaCAGTTCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAg < 1:3345014/70‑1 (MQ=255) aaCAGTTCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAg < 1:2066154/70‑1 (MQ=255) aaCAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGa > 1:2563460/1‑71 (MQ=255) aaCAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGa > 1:811437/1‑71 (MQ=255) aCAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGa < 1:321880/70‑1 (MQ=255) aGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGaaa < 1:3806352/66‑1 (MQ=255) gTTCATTGCGAAACCATCTAGGCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACAtt < 1:3246317/71‑1 (MQ=255) gTTCATTGCGAAACCATCTAGCCAACAAATGCTaa < 1:1764896/35‑1 (MQ=255) gTTCATTGCGAAACCATCTAGCCAACAAATGCTaa < 1:2750987/35‑1 (MQ=255) gTTCATTGCGAAACCATCTAGCCAACAAATGCTaa < 1:3882599/35‑1 (MQ=255) ttCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATg < 1:2145094/61‑1 (MQ=255) ttCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTa < 1:155444/71‑1 (MQ=255) ttCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTa < 1:950087/71‑1 (MQ=255) ttCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTa < 1:38225/71‑1 (MQ=255) ttCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTa < 1:1709006/71‑1 (MQ=255) ttCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTa < 1:3730440/71‑1 (MQ=255) tCATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGtt > 1:119456/1‑40 (MQ=255) cATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACaaa > 1:1266385/1‑57 (MQ=255) cATTGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACaaa > 1:3664725/1‑57 (MQ=255) cATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAAc > 1:587632/1‑71 (MQ=255) cATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAAc > 1:1148066/1‑71 (MQ=255) cATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAAc > 1:2375436/1‑71 (MQ=255) cATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAAc > 1:130934/1‑71 (MQ=255) cATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAAc > 1:2324922/1‑71 (MQ=255) aTTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAAc < 1:386130/70‑1 (MQ=255) tgggAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACctc > 1:3269884/4‑71 (MQ=255) tGCGAAACCATCTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGa > 1:2348934/1‑57 (MQ=255) cGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACctct < 1:2776095/70‑1 (MQ=255) cGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCtctc < 1:93215/71‑1 (MQ=255) aCCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCt < 1:2800911/71‑1 (MQ=255) ccATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGaaa < 1:1758008/52‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACAtt < 1:2795593/55‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACAtt < 1:1447891/55‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACAtt < 1:1359974/55‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACAtt < 1:1748927/55‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACAtt < 1:2573836/55‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACctct < 1:2403612/62‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACctct < 1:627849/62‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACctct < 1:3020833/62‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACctct < 1:2727383/62‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACctc < 1:3011263/61‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTc < 1:1228512/69‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTc < 1:260013/69‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTc < 1:2000915/69‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGAACACAAATGAAAGa < 1:702191/51‑1 (MQ=255) tCTAGCCAACAAATGCTAAATTGGTGCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTc < 1:2879711/69‑1 (MQ=255) cTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGa > 1:1046687/1‑50 (MQ=255) cTAGCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGa > 1:3904794/1‑50 (MQ=255) cTAGCAGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGa > 1:3900804/1‑50 (MQ=255) gCCGACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACAtt < 1:615662/51‑1 (MQ=255) gCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGaaa > 1:684614/1‑45 (MQ=255) gCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGaaa > 1:3967884/1‑45 (MQ=255) gCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGaaa > 1:1346000/1‑45 (MQ=255) | CCCACACGAAAGATCACATACAAAGAAAAATTTGTTTATTAACAGTTCATTGCGAAACCATCTAGCCAACAAATGCTAAATTGGTTCGCAATCAGGTACACAAATGAAAGACATTAACCTCTCACCTTC > W3110S.gb/1804310‑1804438 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |