| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,824,257 | T→C | 40.4% | F29S (TTT→TCT) | nadE → | NAD synthetase, NH3/glutamine‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,824,257 | 0 | T | C | 40.4% | 28.1 / 61.4 | 52 | F29S (TTT→TCT) | nadE | NAD synthetase, NH3/glutamine‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (16/15); new base C (13/8); total (29/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGAC > W3110S.gb/1824193‑1824327 | ataaAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGa < 1:3873346/70‑1 (MQ=255)ataaAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGa < 1:1380566/70‑1 (MQ=255) gcTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGaaa < 1:1794191/64‑1 (MQ=255) cAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGaa < 1:811841/55‑1 (MQ=255) cAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCaa > 1:3021488/1‑68 (MQ=255) cAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACt > 1:1211251/1‑71 (MQ=255) aCCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATc > 1:3906816/1‑71 (MQ=255) gCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGt > 1:2872197/1‑71 (MQ=255) gCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGt > 1:722043/1‑71 (MQ=255) cAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGtt < 1:3672005/71‑1 (MQ=255) aGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGtt < 1:2571231/70‑1 (MQ=255) gATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCaa < 1:2720963/58‑1 (MQ=255) ttAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAAc > 1:3394245/1‑58 (MQ=255) tAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATc > 1:1271335/1‑62 (MQ=255) tAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGAAAAg > 1:3943812/1‑45 (MQ=255) aaTGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAg > 1:2109856/1‑44 (MQ=255) aaTGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTa > 1:3373402/1‑47 (MQ=255) aaTGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTa < 1:2568675/70‑1 (MQ=255) aaTGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTa < 1:3190799/70‑1 (MQ=255) aaTGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTa > 1:2686011/1‑70 (MQ=255) aaTGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTa > 1:2618041/1‑70 (MQ=255) aaTGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCAGAAAAGc > 1:2111747/1‑45 (MQ=255) tGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAAt < 1:1596125/71‑1 (MQ=255) tGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGaa < 1:1121415/39‑1 (MQ=255) tGCTGAAGAGGAAATTCGTCGTAGTGTCGATTCTCTGaa < 1:987477/39‑1 (MQ=255) cTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCaag > 1:1510627/1‑50 (MQ=255) agagGAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTa < 1:1405311/62‑1 (MQ=255) gAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCAc < 1:273425/64‑1 (MQ=255) gAAATTCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGc > 1:872684/1‑70 (MQ=255) aTTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTaaa < 1:656154/57‑1 (MQ=255) ttCGTCGTAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTa < 1:2682300/43‑1 (MQ=255) cgtcgtAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATc > 1:1481244/1‑43 (MQ=255) cgtcgtAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATc > 1:3963833/1‑43 (MQ=255) cgtcgtAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATc > 1:647039/1‑43 (MQ=255) cgtcgtAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTATc > 1:3096286/1‑43 (MQ=255) tcgtAGTGTCGATTCTCTGAAAAGCTACCTGCAAACt < 1:369356/37‑1 (MQ=255) gtAGTGTCGATTCTCTGAAAAGCTACCTGCAAACTTa > 1:3781069/1‑37 (MQ=255) aGTGTCGATTTTCTGAAAAGCTACCTGAAAACTTATCCGtt < 1:895864/41‑1 (MQ=255) tgtCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTa < 1:3201794/44‑1 (MQ=255) tCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTg > 1:1535140/1‑50 (MQ=255) tCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTa < 1:2625167/42‑1 (MQ=255) tCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:1555797/1‑71 (MQ=255) tCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:448034/1‑71 (MQ=255) tCGATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:3511087/1‑71 (MQ=255) gATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:2075307/1‑69 (MQ=255) gATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:685494/1‑69 (MQ=255) gATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:3108594/1‑69 (MQ=255) gATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGt > 1:303711/1‑69 (MQ=255) gATTCTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTaaa > 1:3121532/1‑42 (MQ=255) aTTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACt < 1:2939702/46‑1 (MQ=255) aTTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAg < 1:4029052/71‑1 (MQ=255) aTTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAg < 1:933384/71‑1 (MQ=255) ttCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGAc > 1:2307470/1‑71 (MQ=255) | ATAAAGGCGCTGGGCGCAAAACCGCAGATTAATGCTGAAGAGGAAATTCGTCGTAGTGTCGATTTTCTGAAAAGCTACCTGCAAACTTATCCGTTCATTAAATCACTGGTGCTCGGGATCAGCGGCGGTCAGGAC > W3110S.gb/1824193‑1824327 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |