| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,871,291 | A→G | 8.8% | E208G (GAA→GGA) | yeaH → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,871,291 | 0 | A | G | 8.8% | 154.7 / 6.7 | 57 | E208G (GAA→GGA) | yeaH | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (22/30); new base G (2/3); total (24/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
GGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATT > W3110S.gb/1871225‑1871355 | gggAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAagag > 1:3349973/1‑71 (MQ=255) gAACTTCATGCACTGGAGGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGGAGAgg > 1:2470133/1‑70 (MQ=255) ggAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTgcgc > 1:371537/1‑67 (MQ=255) ggAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGACTGAGCa > 1:1565638/1‑68 (MQ=255) tttGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGt > 1:3344814/1‑52 (MQ=255) tttGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGaa < 1:1457080/64‑1 (MQ=255) ccATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGaa < 1:93848/59‑1 (MQ=255) atcatcAGCAACAGTGAACCTGCGCAACTGCTGGGAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACg < 1:961091/71‑1 (MQ=255) atcatcAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACg < 1:3546751/71‑1 (MQ=255) atcatcAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACg < 1:2590226/71‑1 (MQ=255) tcatcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAgg < 1:3337688/39‑1 (MQ=255) tcatcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCaaa < 1:3206131/53‑1 (MQ=255) tcatcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCa < 1:3015698/51‑1 (MQ=255) tcatcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAAt < 1:3843345/66‑1 (MQ=255) tcatcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAAt < 1:1666668/66‑1 (MQ=255) atcaGCAACAGTGAAGCTGCGCAACTGCTGGAAGAGAAACGTCTGCGCAAAGAAATTGCAGAATTACGTGc > 1:3805475/1‑71 (MQ=255) atcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGaa > 1:1787341/1‑39 (MQ=255) atcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGaa > 1:2893561/1‑39 (MQ=255) atcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCaaa > 1:620794/1‑51 (MQ=255) atcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGc > 1:448463/1‑59 (MQ=255) atcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGc > 1:1651888/1‑71 (MQ=255) atcaGCAACAGTGAACATGCGCAACTGCTGGAAGAGGaa > 1:2446426/1‑39 (MQ=255) tcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGa < 1:3914246/52‑1 (MQ=255) tcaGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGa < 1:1607145/52‑1 (MQ=255) aGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGaa > 1:3205970/1‑36 (MQ=255) aGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGaa > 1:4060198/1‑36 (MQ=255) gCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCaaa < 1:4021206/71‑1 (MQ=255) gCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCaaa < 1:2084378/71‑1 (MQ=255) aCAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAAtt > 1:1788810/1‑71 (MQ=255) gTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGa < 1:2461498/43‑1 (MQ=255) gTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGa < 1:1624873/43‑1 (MQ=255) gTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGa < 1:1181148/43‑1 (MQ=255) gTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGa < 1:3396126/43‑1 (MQ=255) tGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTg < 1:1105306/68‑1 (MQ=255) ccTGCGCAACTGCTGGGAGAGGAACGTCTGCGCAAAGAAATTGCAGAAt < 1:764895/49‑1 (MQ=255) ccTGCGCAACTGCTGGGAGAGGAACGTCTGCGCAAAGAAATTGCAGAAt < 1:1472610/49‑1 (MQ=255) ccTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAAc > 1:1981644/1‑67 (MQ=255) ccTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAAc > 1:1098210/1‑67 (MQ=255) ccTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAAc > 1:1688400/1‑67 (MQ=255) ccTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAAc > 1:3736672/1‑67 (MQ=255) tGCGCAACTGCTGGGAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTa > 1:3757723/1‑49 (MQ=255) tGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTcc > 1:206400/1‑71 (MQ=255) tGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTcc > 1:3515608/1‑71 (MQ=255) gcgcAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAAcgcg > 1:3880955/1‑67 (MQ=255) gcAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACg < 1:448207/48‑1 (MQ=255) cAACTGCTGGAAGAGGAACGTCTGCTCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCttt < 1:3509249/71‑1 (MQ=255) cAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCttt < 1:1428818/71‑1 (MQ=255) aaCTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCtttt < 1:3511777/71‑1 (MQ=255) gctgGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTAtt > 1:3249707/1‑70 (MQ=255) ctgGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTAtt < 1:1716695/69‑1 (MQ=255) ctgGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTAtt < 1:290544/69‑1 (MQ=255) ctgGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTAtt < 1:394013/69‑1 (MQ=255) ctgGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTAtt < 1:778423/69‑1 (MQ=255) ctgGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTAtt < 1:862020/69‑1 (MQ=255) ctgGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTAtt < 1:2529874/69‑1 (MQ=255) gAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAAtt < 1:4078594/48‑1 (MQ=255) gAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAAtt < 1:2053894/48‑1 (MQ=255) aaGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAAtt < 1:647316/47‑1 (MQ=255) | GGGAACTTCATGCACTGGAAGAGAATTTGGCCATCATCAGCAACAGTGAACCTGCGCAACTGCTGGAAGAGGAACGTCTGCGCAAAGAAATTGCAGAATTACGTGCCAAAATTGAACGCGTCCCTTTTATT > W3110S.gb/1871225‑1871355 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |