BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	W3110S.gb	1,885,888	A→G	13.0%	T329T (ACA→ACG)	yeaV →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,885,888	0	A	G	13.0%	112.9 / 9.0	46	T329T (ACA→ACG)	yeaV	predicted transporter
Reads supporting (aligned to +/- strand): ref base A (22/18); new base G (4/2); total (26/20)
Fisher's exact test for biased strand distribution p-value = 6.84e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGC  >  W3110S.gb/1885823‑1885953

aTGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACGCTgg                                                               >  1:1844193/1‑70 (MQ=255)

   gAAAGCTATGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGggcgg                                                           >  1:4071330/1‑71 (MQ=255)

   gAAAGCTATGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGggcgg                                                           >  1:4076407/1‑71 (MQ=255)

         tATGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGaaa                                                      >  1:1447635/1‑70 (MQ=255)

             ccATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGc                                                  <  1:362096/70‑1 (MQ=255)

             ccATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGc                                                  <  1:75910/70‑1 (MQ=255)

             ccATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGc                                                  <  1:558065/70‑1 (MQ=255)

             ccATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGc                                                  <  1:1507062/70‑1 (MQ=255)

               aTTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAaca                                                                   >  1:1441159/1‑51 (MQ=255)

                ttCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACGCTGGGCGGCGAAACAGCTGTa                                              <  1:246219/71‑1 (MQ=255)

                ttCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACGCTGGGCGGCGAAACAGCTGTa                                              <  1:1241951/71‑1 (MQ=255)

                ttCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGc                                                  <  1:3429439/67‑1 (MQ=255)

                ttCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGc                                                  <  1:2154783/67‑1 (MQ=255)

                ttCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGc                                                  <  1:1244906/67‑1 (MQ=255)

                ttCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGc                                                  <  1:1234240/67‑1 (MQ=255)

                 tcatcaGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTa                                              >  1:3989124/1‑70 (MQ=255)

                  catcaGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAaca                                                                   >  1:394152/1‑48 (MQ=255)

                  catcaGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAaca                                                                   >  1:3127341/1‑48 (MQ=255)

                  catcaGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTg                                                >  1:1377765/1‑67 (MQ=255)

                  catcaGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTg                                                >  1:2172094/1‑67 (MQ=255)

                  catcaGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTa                                              >  1:1199660/1‑69 (MQ=255)

                  catcaGATTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAaca                                                                   >  1:8742/1‑48 (MQ=255)

                          tATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAacac                                                                  >  1:3301303/1‑41 (MQ=255)

                          tATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGtt                                     >  1:3904183/1‑70 (MQ=255)

                          tATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTc                                    >  1:3731920/1‑71 (MQ=255)

                            tCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTg                                  <  1:2028140/71‑1 (MQ=255)

                                tGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGt                              >  1:1008400/1‑71 (MQ=255)

                                  gCGTAATCAACGTCCCACAGGTGCTGGAGACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCg                            >  1:2372386/1‑71 (MQ=255)

                                  gCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTc                                    >  1:2201679/1‑63 (MQ=255)

                                  gCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTc                                    >  1:457356/1‑63 (MQ=255)

                                    gTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTg                                                <  1:1738787/49‑1 (MQ=255)

                                    gTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTg                                                <  1:3824698/49‑1 (MQ=255)

                                       aTCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGc                        >  1:2871644/1‑70 (MQ=255)

                                         cAACGTCCCACAGGTGCTGGAAACGCTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGa                                 >  1:1174990/1‑59 (MQ=255)

                                          aaCGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGc                    >  1:3342432/1‑71 (MQ=255)

                                                ccACAGGTGCTGGAAACGCTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTa               >  1:2823600/1‑70 (MQ=255)

                                                ccACAGGTGCTGGAAACGCTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTa               >  1:3378871/1‑70 (MQ=255)

                                                ccACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTa               >  1:3206816/1‑70 (MQ=255)

                                                ccACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTa               >  1:760586/1‑70 (MQ=255)

                                                ccACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTa               >  1:451618/1‑70 (MQ=255)

                                                ccACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTa               >  1:1426335/1‑70 (MQ=255)

                                                  acaGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAAt            <  1:2356072/71‑1 (MQ=255)

                                                  acaGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAAt            <  1:1642024/71‑1 (MQ=255)

                                                  acaGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAAt            <  1:1432748/71‑1 (MQ=255)

                                                  acaGGTCCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAAt            <  1:3198094/71‑1 (MQ=255)

                                                     ggTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGt         <  1:3171482/71‑1 (MQ=255)

                                                      gTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGtt        >  1:3889030/1‑71 (MQ=255)

                                                           ggAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTcg   >  1:1948834/1‑71 (MQ=255)

                                                            gAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTcgc  >  1:670048/1‑71 (MQ=255)

                                                              aaCACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTg          <  1:2889277/61‑1 (MQ=255)

                                                              aaCACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTg          <  1:11232/61‑1 (MQ=255)

ATGGAAAGCTATGCCATTCATCAGTTTATCAATGGCGTAATCAACGTCCCACAGGTGCTGGAAACACTGGGCGGCGAAACAGCTGTACAGCAAGTTCTGATGTCGTTGCCAGCCGGTAAATTGTTCCTCGC  >  W3110S.gb/1885823‑1885953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`