| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,915,303 | A→G | 48.5% | F410L (TTT→CTT) | prc ← | carboxy‑terminal protease for penicillin‑binding protein 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,915,303 | 0 | A | G | 48.5% | ‑5.3 / 46.2 | 33 | F410L (TTT→CTT) | prc | carboxy‑terminal protease for penicillin‑binding protein 3 |
| Reads supporting (aligned to +/- strand): ref base A (4/13); new base G (8/8); total (12/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01 | |||||||||||
ATCGCTATCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCATTGCTACGCAGGTCGATGAT > W3110S.gb/1915238‑1915366 | aTCGCTATCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGAc > 1:2121064/1‑71 (MQ=255) gCTATCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAGCAGACCg > 1:3318240/1‑70 (MQ=255) cACGATCCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGa < 1:1858097/70‑1 (MQ=255) cACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGa < 1:1524324/70‑1 (MQ=255) cGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATAc < 1:3727707/71‑1 (MQ=255) cGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATAc < 1:3202854/71‑1 (MQ=255) gCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAGCAGACCGGAGAGCGATACGGCTTCAg > 1:3902526/1‑71 (MQ=255) cGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAGCAGACCGGAGAGCGATACGGCTTCAGtt < 1:1184017/71‑1 (MQ=255) cGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAGCAGACCGGAGAGCGATACGGCTTCAGtt < 1:4046265/71‑1 (MQ=255) cGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAGCAGACCGGAGAGCGATACGGCTTCAGtt < 1:3903202/71‑1 (MQ=255) ttgttATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGTCTTCAGt < 1:968468/68‑1 (MQ=255) ttgttATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGTCTTCAGt < 1:963610/68‑1 (MQ=255) tgttATCGCGGACCTGAACAATGGGACCCGCAGGAATAAGCAGACCGGAGAGCGATACGGCTTCAGTTAAc > 1:211207/1‑71 (MQ=255) aTCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGccc > 1:816808/1‑71 (MQ=255) aCCTGAACAATGGGACCCGCAGGAATAAGCAGACCGGAGAGCGa < 1:950953/44‑1 (MQ=255) aCCTGAACAATGGGACCCGCAGGAATAAGCAGACCGGAGAGCGa < 1:599123/44‑1 (MQ=255) ccTGAACAATGGGACCCGCAGGAATAAACAGACCGTAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCa < 1:2051019/71‑1 (MQ=255) ccTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCa < 1:1272802/71‑1 (MQ=255) gAACAATGGGACCCGCAGGAATAAGCAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCATTg > 1:3554/1‑71 (MQ=255) aaCAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACTGCCAtt < 1:527478/69‑1 (MQ=255) aaCAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCATTGc < 1:1997421/71‑1 (MQ=255) aaCAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCATTGc < 1:1577391/71‑1 (MQ=255) cAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAg > 1:2044046/1‑48 (MQ=255) aaTGGGACCCGCAGGAATAAGCAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGc < 1:2613303/62‑1 (MQ=255) aaTGGGACCCGCAGGAATAAGCAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGc < 1:1603366/62‑1 (MQ=255) tGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCATTGCTACg > 1:3900420/1‑70 (MQ=255) gCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCATTGCTACGCAGGTCgat < 1:1154940/71‑1 (MQ=255) gCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCATTGCTACGCAGGTCgat < 1:2851431/71‑1 (MQ=255) ggAATAAGCAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCATTGCTACGCAGGTCgatgat < 1:1900517/71‑1 (MQ=255) aTAAGCAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCATTGCTACGCAGGTCgatga > 1:55840/1‑67 (MQ=255) aTAAGCAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCATTGCTACGCAGGTCgatga > 1:664609/1‑67 (MQ=255) aTAAGCAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCATTGCTACGCAGGTCgatga > 1:1761109/1‑67 (MQ=255) aTAAGCAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCATTGCTACGCAGGTCgatga > 1:2309445/1‑67 (MQ=255) | ATCGCTATCTTCACGAACCTTGCCGTTGTTATCGCGGACCTGAACAATGGGACCCGCAGGAATAAACAGACCGGAGAGCGATACGGCTTCAGTTAACGCCCCACCGCCATTGCTACGCAGGTCGATGAT > W3110S.gb/1915238‑1915366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |