| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 1,989,605 | G→A | 16.0% | intergenic (+40/+39) | yecI → / ← yecJ | predicted ferritin‑like protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,989,605 | 0 | G | A | 16.0% | 59.7 / 5.1 | 25 | intergenic (+40/+39) | yecI/yecJ | predicted ferritin‑like protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (8/13); new base A (2/2); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTG > W3110S.gb/1989539‑1989674 | aaTGTCGTGTCACACCAGCTTCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTgag < 1:1255453/69‑1 (MQ=255)aaTGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTgag < 1:406778/69‑1 (MQ=255)aaTGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTaaga < 1:3047300/70‑1 (MQ=255) tCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTAAGAAAcc < 1:696914/70‑1 (MQ=255) gtgtCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCt < 1:3589646/71‑1 (MQ=255) cAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCt < 1:2958615/71‑1 (MQ=255) tGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGtt > 1:3393137/1‑65 (MQ=255) gATCATCATCGGCGCTAATGCATTGTGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAagc < 1:1013049/70‑1 (MQ=255) gATCATCATCGGCGCTAATGCATTGTGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAagc < 1:1008179/70‑1 (MQ=255) gATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAag > 1:4058984/1‑69 (MQ=255) catcGGCGCTAATGCATTGCGCCGATGAAGGTTTTAAGAAACCGCTGCCt > 1:1370577/1‑50 (MQ=255) catcGGCGCTAATGCATTGCGCCGATGAAGGTTTTAAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGtt > 1:2477422/1‑71 (MQ=255) tcGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGtttt < 1:395522/71‑1 (MQ=255) gcgcTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGttttttt < 1:1573748/71‑1 (MQ=255) aTGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATgg < 1:1917662/70‑1 (MQ=255) tGCATTGCTCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGtt > 1:4051256/1‑46 (MQ=255) cATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAag > 1:2474934/1‑49 (MQ=255) cATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGAtt < 1:2937838/71‑1 (MQ=255) cATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGAtt < 1:1314361/71‑1 (MQ=255) cATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGAt > 1:501582/1‑70 (MQ=255) cATTGCGCCGATGAAGGTTTTAAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGAtt < 1:1239570/71‑1 (MQ=255) ttGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATggg < 1:1570006/66‑1 (MQ=255) ttGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATggg < 1:2511714/66‑1 (MQ=255) gcgcCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGAt > 1:2281078/1‑66 (MQ=255) gcgcCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGAt > 1:2276417/1‑66 (MQ=255) gcCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATgg < 1:2545760/61‑1 (MQ=255) aaGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGttttttt < 1:2008781/49‑1 (MQ=255) ttGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACtt > 1:3728501/1‑71 (MQ=255) gagaAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTg < 1:3543444/70‑1 (MQ=255) gagaAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTg < 1:2121473/70‑1 (MQ=255) | AATGTCGTGTCACACCAGCTGCATTGATCATCATCGGCGCTAATGCATTGCGCCGATGAAGGTTTTGAGAAACCGCTGCCTCATCTGTTTGAAGCAGCGGTTTTTTTAATGGGATTCACCCTGTGGGGTAAACTTG > W3110S.gb/1989539‑1989674 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |