| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,045,238 | A→G | 25.0% | intergenic (+203/+367) | yodB → / ← serU | predicted cytochrome/tRNA‑Ser |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,045,238 | 0 | A | G | 25.0% | 53.7 / 17.1 | 32 | intergenic (+203/+367) | yodB/serU | predicted cytochrome/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base A (15/9); new base G (2/6); total (17/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCTGA > W3110S.gb/2045170‑2045300 | aTCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCaaa > 1:2155773/1‑70 (MQ=255) tCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCaaaa > 1:841730/1‑70 (MQ=255) aaaaaTACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAGAAAAg > 1:625856/1‑71 (MQ=255) tACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCg < 1:258110/71‑1 (MQ=255) tAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTg > 1:895879/1‑62 (MQ=255) tAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTg > 1:519179/1‑62 (MQ=255) tAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTc > 1:3704008/1‑70 (MQ=255) gTATTTAAACCTTTTTTACTTGTTAGTATGATATAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAActc < 1:2731130/70‑1 (MQ=255) gTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAActc < 1:2556849/70‑1 (MQ=255) gTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAAct < 1:2912965/69‑1 (MQ=255) tATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAActc < 1:4084543/69‑1 (MQ=255) tttAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAGAAAAGCTGCGAAATTcaaca < 1:3930516/63‑1 (MQ=255) tAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAGAAAAGCTGCGAAATTCAACAACTCTTGGa < 1:1883953/70‑1 (MQ=255) tAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTgg > 1:2793777/1‑69 (MQ=255) tAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTgg > 1:3405190/1‑69 (MQ=255) tAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGa > 1:427230/1‑70 (MQ=255) tAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGa < 1:1353745/70‑1 (MQ=255) tAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGa < 1:3687299/70‑1 (MQ=255) tAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGa > 1:3454354/1‑70 (MQ=255) tAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGa > 1:3276409/1‑70 (MQ=255) tAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGa > 1:691262/1‑70 (MQ=255) tAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGa < 1:2348580/70‑1 (MQ=255) ccTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAGAAAAGCTGCGAAATTCAACAACTCTTGGAAATTa < 1:1668078/71‑1 (MQ=255) tttttACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATg > 1:3635476/1‑70 (MQ=255) aCTTGTTAGTATGATACAAAGGCTTTCAGAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACt < 1:2134052/69‑1 (MQ=255) tGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTa > 1:144856/1‑52 (MQ=255) tGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTa > 1:2283630/1‑52 (MQ=255) tGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCt > 1:297966/1‑71 (MQ=255) tACAAAGGCTTTCAGAAAAGCTGCTAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCttt < 1:507144/70‑1 (MQ=255) tACAAAGGCTTTCAGAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCttt < 1:1338095/70‑1 (MQ=255) tACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTa < 1:245345/71‑1 (MQ=255) aaGGCTTTCAGAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCcg > 1:3103783/1‑59 (MQ=255) aaGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCt > 1:1073292/1‑71 (MQ=255) ggCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCTGa > 1:1401416/1‑71 (MQ=255) | ATCAAAAATACAGGTTAATTTTGGTATTTAAACCTTTTTTACTTGTTAGTATGATACAAAGGCTTTCAAAAAAGCTGCGAAATTCAACAACTCTTGGAAATTATGGACTTTGTCCCGCGGCTTTAATCTGA > W3110S.gb/2045170‑2045300 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |