| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,081,459 | A→G | 25.4% | F36L (TTT→CTT) | yeeX ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,081,459 | 0 | A | G | 25.4% | 115.3 / 43.8 | 67 | F36L (TTT→CTT) | yeeX | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (20/30); new base G (11/6); total (31/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.64e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01 | |||||||||||
ACGGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTCGGA > W3110S.gb/2081391‑2081528 | aCGGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAAt < 1:2565719/70‑1 (MQ=255) gATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCa < 1:557383/71‑1 (MQ=255) tCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTcc < 1:324786/68‑1 (MQ=255) cTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTa > 1:1317883/1‑71 (MQ=255) tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAg < 1:1067431/66‑1 (MQ=255) tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAg < 1:1339754/66‑1 (MQ=255) tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAg < 1:3116191/66‑1 (MQ=255) tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACg < 1:3425698/70‑1 (MQ=255) tttCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACg < 1:2944437/70‑1 (MQ=255) aaCGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTgg < 1:561689/70‑1 (MQ=255) aaCGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTgg < 1:789766/70‑1 (MQ=255) gTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATg < 1:3854304/71‑1 (MQ=255) tCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTgg < 1:3384534/66‑1 (MQ=255) tCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTgg < 1:2035175/66‑1 (MQ=255) tCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTgg < 1:3092732/66‑1 (MQ=255) tCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTgg < 1:139713/66‑1 (MQ=255) cTGAATTTCACGTTGCAGTTTGTTCTTACGACTGAACAGACGAACAAGTTCCAGTACGTCCTGGaa > 1:2913176/1‑66 (MQ=255) cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGaa > 1:2607045/1‑66 (MQ=255) cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGaa > 1:2873234/1‑66 (MQ=255) cTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAg > 1:343482/1‑71 (MQ=255) gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:1924645/1‑71 (MQ=255) gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:3468426/1‑71 (MQ=255) gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:2637278/1‑71 (MQ=255) gAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGc > 1:1551449/1‑71 (MQ=255) aaTTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGc < 1:2620452/70‑1 (MQ=255) aTTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGaa < 1:1996615/66‑1 (MQ=255) tttCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATACCAGTACGTCCTGGAATGAAGGCTTg > 1:1313302/1‑71 (MQ=255) ttCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAgg > 1:421947/1‑66 (MQ=255) tCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTgg > 1:2905752/1‑70 (MQ=255) cACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTgg > 1:958428/1‑69 (MQ=255) cACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGt < 1:2673395/70‑1 (MQ=255) cACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGt < 1:797846/70‑1 (MQ=255) cACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGt < 1:1600622/70‑1 (MQ=255) aCGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTa < 1:1291019/70‑1 (MQ=255) cGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt < 1:1167809/71‑1 (MQ=255) cGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt < 1:2564978/71‑1 (MQ=255) cGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt < 1:1692229/71‑1 (MQ=255) cGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt < 1:81412/71‑1 (MQ=255) cGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGt < 1:913385/71‑1 (MQ=255) gTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtt < 1:1412459/71‑1 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:1072032/1‑70 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:709424/1‑70 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:179184/1‑70 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:1453916/1‑70 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTa > 1:1146383/1‑36 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:3732136/1‑70 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:989196/1‑70 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt > 1:3129350/1‑70 (MQ=255) tGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:2797259/70‑1 (MQ=255) gTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta > 1:1546811/1‑71 (MQ=255) gTTCTTACGACGGAACAGACGAACAAATTACAGTACGTCCTGGAATGAAGGCTTgg < 1:1907242/56‑1 (MQ=255) ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtt < 1:3722542/66‑1 (MQ=255) ttCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTtat < 1:2682228/71‑1 (MQ=255) ttCTTACGACGAAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:965175/70‑1 (MQ=255) cTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATAc > 1:1627781/1‑71 (MQ=255) acgGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:1417679/60‑1 (MQ=255) gAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:1848622/57‑1 (MQ=255) gAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATaa > 1:1851749/1‑71 (MQ=255) gAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATaa > 1:316456/1‑71 (MQ=255) gAACAGACGAACAAATTCCAGTAAGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATaa > 1:3938316/1‑71 (MQ=255) cAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCAtttt < 1:3577297/54‑1 (MQ=255) aGACGAACAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAAtgt > 1:2753974/1‑70 (MQ=255) gACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAAtgt > 1:722243/1‑69 (MQ=255) gAACAAATTGCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGagag < 1:845507/71‑1 (MQ=255) cAAGTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTc > 1:1319360/1‑70 (MQ=255) cAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta > 1:1041201/1‑48 (MQ=255) cAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAAt > 1:27136/1‑61 (MQ=255) aaaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAAtgtg < 1:774934/63‑1 (MQ=255) aaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:2422114/46‑1 (MQ=255) aaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTta < 1:1412823/46‑1 (MQ=255) aaTTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTCGGa > 1:3109978/1‑71 (MQ=255) | ACGGATCTTTTTCTCAACGTCCTGAATTTCACGTTGCAGTTTGTTCTTACGACGGAACAGACGAACAAATTCCAGTACGTCCTGGAATGAAGGCTTGGTAGTTTCCATTTTTATACCCCTGATAATGTGAGAGTCGGA > W3110S.gb/2081391‑2081528 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |