| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,096,084 | G→A | 17.5% | E160E (GAG→GAA) | hisB → | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,096,084 | 0 | G | A | 17.5% | 237.9 / 38.6 | 97 | E160E (GAG→GAA) | hisB | fused histidinol‑phosphatase and imidazoleglycerol‑phosphate dehydratase |
| Reads supporting (aligned to +/- strand): ref base G (44/36); new base A (10/7); total (54/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.84e-01 | |||||||||||
GCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGG > W3110S.gb/2096016‑2096154 | gCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTTAACTGGCCAATGATTGGCGAGc > 1:1508763/1‑70 (MQ=255)gCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGc < 1:3101950/70‑1 (MQ=255) cGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCaa < 1:2855509/71‑1 (MQ=255) gAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACt < 1:934243/71‑1 (MQ=255) gAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACt < 1:3694052/71‑1 (MQ=255) aaaaCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTc < 1:293769/71‑1 (MQ=255) aaaaCATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTc < 1:3626748/71‑1 (MQ=255) ggCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCCCCAGACg > 1:3353941/1‑71 (MQ=255) ggCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACg > 1:3846525/1‑71 (MQ=255) ggCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACg > 1:1501721/1‑71 (MQ=255) cATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTg < 1:2938738/71‑1 (MQ=255) cATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTg < 1:2046374/71‑1 (MQ=255) cATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTg < 1:547055/71‑1 (MQ=255) ttACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGc < 1:2704535/54‑1 (MQ=255) ttACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTc < 1:1127720/59‑1 (MQ=255) ttACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTc < 1:873724/59‑1 (MQ=255) tACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAcc > 1:4017690/1‑71 (MQ=255) tACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGAcc > 1:1559865/1‑71 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCaa > 1:3873949/1‑54 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGa > 1:4006272/1‑63 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGa > 1:452580/1‑63 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCg > 1:679025/1‑71 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCg > 1:2499935/1‑71 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCg > 1:2610890/1‑71 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCg > 1:3681184/1‑71 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg > 1:3435335/1‑71 (MQ=255) aCTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCg > 1:771564/1‑71 (MQ=255) aCTGGATTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCa > 1:660422/1‑61 (MQ=255) cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:1286044/71‑1 (MQ=255) cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:866611/71‑1 (MQ=255) cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:3794796/71‑1 (MQ=255) cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:2537943/71‑1 (MQ=255) cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt < 1:3918839/71‑1 (MQ=255) cTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACAGt < 1:2229832/71‑1 (MQ=255) tACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt > 1:2719082/1‑71 (MQ=255) tACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCt > 1:949044/1‑71 (MQ=255) gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCAc < 1:3273273/71‑1 (MQ=255) gCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCAc < 1:2892866/71‑1 (MQ=255) cTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACg < 1:3292073/71‑1 (MQ=255) tACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGt > 1:1128969/1‑60 (MQ=255) tACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACg > 1:2102778/1‑70 (MQ=255) tACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACg > 1:1585634/1‑70 (MQ=255) cGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGt < 1:3337144/69‑1 (MQ=255) ccGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGc > 1:2133557/1‑71 (MQ=255) ccGCGAAACCCTGAACTGGCCAATGATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGc > 1:3285015/1‑71 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:4065123/71‑1 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:269000/71‑1 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:3774575/71‑1 (MQ=255) gcgAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGt < 1:3855146/71‑1 (MQ=255) cTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCa > 1:3669482/1‑70 (MQ=255) cTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCa > 1:1650420/1‑70 (MQ=255) cTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCa > 1:59784/1‑70 (MQ=255) gAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCa > 1:559101/1‑68 (MQ=255) aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa < 1:2804414/62‑1 (MQ=255) aaCTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTaa < 1:1481263/62‑1 (MQ=255) aaCTGGCCAATGATTGGAGAGCAACTCACCAGACGTGACCGTTACGCACACGTAGTGCGTAATACCAAaga < 1:159220/71‑1 (MQ=255) cTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATa > 1:138061/1‑62 (MQ=255) cTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATa > 1:2233017/1‑62 (MQ=255) ggCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg > 1:3133475/1‑71 (MQ=255) ggCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg > 1:128397/1‑71 (MQ=255) ggCCAATGATTGGCGAGCAACTAACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg > 1:1099058/1‑71 (MQ=255) ccAATGATTGGCGAGCAACTCACCAGACGTGACCGtt < 1:1886898/37‑1 (MQ=255) ccAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAaga < 1:128264/65‑1 (MQ=255) aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTAc > 1:3660851/1‑37 (MQ=255) aaTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACg > 1:4022449/1‑67 (MQ=255) aaTGATTGGCGAGCAACTCACCAGACGTGACAGTTAc > 1:1862070/1‑37 (MQ=255) aTGATTGGCGAGCAAGTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCa < 1:4035262/68‑1 (MQ=255) aTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTATTGCGt < 1:1661626/51‑1 (MQ=255) aTGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCa < 1:2452570/68‑1 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACg < 1:76054/42‑1 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACg < 1:753584/42‑1 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACg < 1:276108/42‑1 (MQ=255) tGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACg < 1:1334278/42‑1 (MQ=255) cgATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACg < 1:3983846/41‑1 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:605710/1‑70 (MQ=255) gATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2948666/1‑70 (MQ=255) gATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:2571198/1‑70 (MQ=255) gATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:704206/1‑70 (MQ=255) gATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3972681/1‑70 (MQ=255) gATTGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGAtt > 1:3403342/1‑70 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTa > 1:1343146/1‑42 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAgaga > 1:3921756/1‑61 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAgaga > 1:1992085/1‑61 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTg < 1:381081/70‑1 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1319771/1‑71 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1849906/1‑71 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:2260572/1‑71 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:2034704/1‑71 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:3268712/1‑71 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:3301453/1‑71 (MQ=255) aTTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGa > 1:1941921/1‑71 (MQ=255) tGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGAc > 1:530640/1‑70 (MQ=255) tGGCGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACg > 1:1713279/1‑71 (MQ=255) ggCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGt > 1:1455695/1‑71 (MQ=255) cGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTc < 1:2313776/71‑1 (MQ=255) cGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGAAGCAGATTGACGTTc < 1:250768/71‑1 (MQ=255) cGAACAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGAAGCAGATTGACGTTc < 1:245969/71‑1 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:579718/71‑1 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:3063202/71‑1 (MQ=255) gCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAgg < 1:1219482/71‑1 (MQ=255) | GCGGAAAACATGGGCATTACTGGTTTACGCTACGACCGCGAAACCCTGAACTGGCCAATGATTGGCGAGCAACTCACCAGACGTGACCGTTACGCTCACGTAGTGCGTAATACCAAAGAGACGCAGATTGACGTTCAGG > W3110S.gb/2096016‑2096154 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |