| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 75,677 | A→C | 23.9% | F541L (TTT→TTG) | sgrR ← | DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 75,677 | 0 | A | C | 23.9% | 51.0 / 34.6 | 46 | F541L (TTT→TTG) | sgrR | DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (20/15); new base C (8/3); total (28/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.86e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.11e-01 | |||||||||||
GGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAG > W3110S.gb/75609‑75746 | ggCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTaaa < 1:2987271/71‑1 (MQ=255)ggCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTaaa < 1:957833/71‑1 (MQ=255)ggCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTaaa < 1:2898920/71‑1 (MQ=255)ggCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTaaa < 1:1740588/71‑1 (MQ=255) tagtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAg > 1:1075109/1‑71 (MQ=255) gtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGcc > 1:674477/1‑71 (MQ=255) gtgATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGcc > 1:1368802/1‑71 (MQ=255) gATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGcc > 1:3476944/1‑69 (MQ=255) gATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGcc > 1:1564170/1‑69 (MQ=255) gATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGcc > 1:3381735/1‑69 (MQ=255) ttttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGa < 1:3169166/69‑1 (MQ=255) tttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTaaaa > 1:1453418/1‑55 (MQ=255) ttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAggg < 1:1724905/70‑1 (MQ=255) ttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAggg < 1:188837/70‑1 (MQ=255) ttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAggg > 1:2133658/1‑70 (MQ=255) ttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAggg < 1:228499/70‑1 (MQ=255) ttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAggg > 1:408769/1‑70 (MQ=255) ttGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCAAGCTGATTTAAAATCGAACCAGCCGAggg < 1:3810708/70‑1 (MQ=255) ttATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTAtt < 1:124722/71‑1 (MQ=255) aTCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTa > 1:1302610/1‑67 (MQ=255) aaCCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTaaaa > 1:2600524/1‑46 (MQ=255) aaCCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTaaaa > 1:2311334/1‑46 (MQ=255) gCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCa > 1:1306164/1‑63 (MQ=255) gCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCa > 1:4022976/1‑63 (MQ=255) gCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCa > 1:4027799/1‑63 (MQ=255) tCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCg < 1:509877/40‑1 (MQ=255) tCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCg < 1:1405015/40‑1 (MQ=255) cATGGATCCGGTGGCGCAAACCACGCTGATTTaaa < 1:764817/35‑1 (MQ=255) cATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcgcg > 1:2752212/1‑71 (MQ=255) aTGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAAc > 1:1819758/1‑41 (MQ=255) aTGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAAc > 1:3333361/1‑41 (MQ=255) aTGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAAc > 1:3412211/1‑41 (MQ=255) aTGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAAc > 1:1316399/1‑41 (MQ=255) aTGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcgcg > 1:856150/1‑70 (MQ=255) tGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCa < 1:2729643/71‑1 (MQ=255) tGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCa < 1:3660938/71‑1 (MQ=255) gATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGTGTATTCATGCGCAGGCCGCGCATa > 1:3420908/1‑71 (MQ=255) gATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATa > 1:1088855/1‑71 (MQ=255) gATCCGGTGGCGCAAACCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATa > 1:2704860/1‑71 (MQ=255) cGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGc > 1:1895878/1‑71 (MQ=255) gTGGCGCAAACCACGCTGATTTAAAATCGAACCAGcc < 1:3408243/37‑1 (MQ=255) ggCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTg > 1:3878749/1‑71 (MQ=255) ggCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTg > 1:710941/1‑71 (MQ=255) gcgcAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCa > 1:868845/1‑45 (MQ=255) gcgcAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCa > 1:873933/1‑45 (MQ=255) aaCCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTg < 1:315925/71‑1 (MQ=255) aaCCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTg < 1:2730286/71‑1 (MQ=255) aaCCACGCTGATTTCAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTg < 1:1534135/71‑1 (MQ=255) cGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCa < 1:3055638/50‑1 (MQ=255) cGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGa < 1:236489/71‑1 (MQ=255) cGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGa < 1:1898223/71‑1 (MQ=255) cTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCcgc < 1:67996/45‑1 (MQ=255) tttAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGc > 1:2991468/1‑70 (MQ=255) tAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAg > 1:3906004/1‑71 (MQ=255) | GGCGTTAGTGTAGTGATTTTGTTATCAACCAGCAATCATGGATCCGGTGGCGCAAACCACGCTGATTTAAAATCGAACCAGCCGAGGGTATTCATGCGCAGGCCGCGCATACTGCGTTGCCCCTGAATGATCAGCCAG > W3110S.gb/75609‑75746 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |