| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,215,186 | A→G | 25.0% | V115V (GTT→GTC) | yehS ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,215,186 | 0 | A | G | 25.0% | 56.6 / 20.9 | 36 | V115V (GTT→GTC) | yehS | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (7/20); new base G (4/5); total (11/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.09e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.29e-01 | |||||||||||
ATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAACGCGATGCGTA > W3110S.gb/2215116‑2215253 | aTTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGaa < 1:3336084/71‑1 (MQ=255) gccgcATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCgg < 1:1963998/70‑1 (MQ=255) cgcgGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAActgctg > 1:511663/1‑70 (MQ=255) cgcgGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGt > 1:1605915/1‑71 (MQ=255) cgcgGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGt > 1:1851109/1‑71 (MQ=255) cgcgGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAAcgc > 1:1692011/1‑60 (MQ=255) tttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAg < 1:431903/71‑1 (MQ=255) tttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAg < 1:4041952/71‑1 (MQ=255) tttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAg < 1:776539/71‑1 (MQ=255) tttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAg < 1:519970/71‑1 (MQ=255) tttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAg < 1:3667955/71‑1 (MQ=255) ttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAg > 1:2947040/1‑70 (MQ=255) ttttATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAg > 1:2494195/1‑70 (MQ=255) aTGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATa < 1:2244503/70‑1 (MQ=255) tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg < 1:1178010/71‑1 (MQ=255) tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGGTGAGGATAGCCAg < 1:2981578/71‑1 (MQ=255) tCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAg < 1:4026014/71‑1 (MQ=255) ggTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAAt < 1:2756075/71‑1 (MQ=255) tGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAg < 1:2752471/66‑1 (MQ=255) gcgcgcATCATCGCTGTAATTTCCGGCATCGAGACGCGGAACTGCTGTTCGATGAGGATAGCCAGAATGt > 1:1895770/1‑70 (MQ=255) gcgcgcATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAg < 1:3757759/65‑1 (MQ=255) cgcATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATc > 1:580308/1‑71 (MQ=255) tcatcGCTGTAATTTCCGGCATCGAAACGCTGAACTGCTGTTCGGt < 1:2004220/46‑1 (MQ=255) tcatcGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGt < 1:1287660/46‑1 (MQ=255) tcatcGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGt < 1:3743225/46‑1 (MQ=255) tcatcGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGtt < 1:165023/71‑1 (MQ=255) atcGCTGTAATTTCCGGCATCGAAACGCGGAACTGTTGTTCGGTGAg > 1:2274174/1‑47 (MQ=255) atcGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGtttt > 1:3036339/1‑71 (MQ=255) tAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAg < 1:2645454/40‑1 (MQ=255) tAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCg > 1:1073041/1‑69 (MQ=255) ttCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAg < 1:3753166/45‑1 (MQ=255) ccGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:3385542/70‑1 (MQ=255) ccGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:643379/70‑1 (MQ=255) ccGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgc < 1:914569/70‑1 (MQ=255) cGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgcg < 1:1772735/64‑1 (MQ=255) cGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAAcgcg < 1:207032/64‑1 (MQ=255) gAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAACGCGATGCGTa < 1:1680317/70‑1 (MQ=255) aaaCGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAACGCGATGCGTa < 1:3986476/69‑1 (MQ=255) | ATTGATCGCCGCATTCGCGGAAGTTTTTATGATCCGGTGCGCGCATCATCGCTGTAATTTCCGGCATCGAAACGCGGAACTGCTGTTCGGTGAGGATAGCCAGAATGTCATCGGTTTTCAGCGAAAACGCGATGCGTA > W3110S.gb/2215116‑2215253 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |